PKN1 antibody (AA 836-870)

Catalog No. ABIN6242529

The Rabbit Polyclonal anti-PKN1 antibody is suitable to detect PKN1 in samples from Human. It has been validated for WB, IHC (p), IF and FACS.

Quick Overview for PKN1 antibody (AA 836-870) (ABIN6242529)

Target: PKN1 (Protein Kinase N1 (PKN1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: RB56692

Product Details anti-PKN1 Antibody

Binding Specificity: AA 836-870

Predicted Reactivity: B, D

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This PKN1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 836-870 amino acids from human PKN1.

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:25. WB: 1:2000. IHC-P: 1:25. FC: 1:25

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

Target Details for PKN1

Target: PKN1 (Protein Kinase N1 (PKN1))

Alternative Name: PKN1

Background: PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser- 159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro.

Molecular Weight: 103932

UniProt: Q16512