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Details for Product No. ABIN968122

Minichromosome Maintenance Complex Component 2 (MCM2) (AA 725-888) antibody

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Antigen
Synonyms
BM28, CCNL1, CDCL1, D3S3194, MITOTIN, cdc19, AA959861, AW476101, Mcmd2, mKIAA0030, bm28, ccnl1, cdcl1, mitotin, CG7538, DmMCM2, DmMcm2, DmeMCM2, Dmel\\CG7538, MCM2, MCM2-7, MCM2_DROME, McM2, PCR3, dMC ... show more
Epitope
»Alternatives AA 725-888
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
Pubmed 5 references available
Catalog no. ABIN968122
Quantity 150 µg
Price
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Immunogen Human BM28
Clone BB10
Isotype IgG1
Cross-Reactivity Chicken, Dog (Canine), Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name BM28
Background BM28 is a phosphoprotein that migrates at 125kDa in SDS PAGE with a hyperphosphorylated form migrating as a slightly slower band. The ratio of the phosphorylation states alters with the phases of cell cycle - in M, the fast phosphorylated BM28 is the major form found, while in G1 phase, the slow, hyperphosphorylated BM28 predominates. The cellular localization of BM28 is also cell cycle dependent. In G1, most of the BM28 is chromatin bound, whereas, by M phase, the BM28 is still nuclear, but not associated with the DNA. BM28 is necessary for both entry into S phase and cell division as determined by microinjection inhibition experiments. Microinjection of a BM28 antibody into synchronised cells in G1 phase inhibits DNA replication. When injected during S phase or later, no effect on DNA replication is seen, but cell division is inhibited.
Molecular Weight 125 kDa
Comment

Related Products: ABIN968536, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Storage -20 °C
Product cited in: Todorov, Attaran, Kearsey: "BM28, a human member of the MCM2-3-5 family, is displaced from chromatin during DNA replication." in: The Journal of cell biology, Vol. 129, Issue 6, pp. 1433-45, 1995 (PubMed).

Todorov, Pepperkok, Philipova et al.: "A human nuclear protein with sequence homology to a family of early S phase proteins is required for entry into S phase and for cell division." in: Journal of cell science, Vol. 107 ( Pt 1), pp. 253-65, 1994 (PubMed).

Ishimi, Ichinose, Omori et al.: "Binding of human minichromosome maintenance proteins with histone H3." in: The Journal of biological chemistry, Vol. 271, Issue 39, pp. 24115-22, 1996 (PubMed).

Cook, Park, Burke et al.: "Analysis of Cdc6 function in the assembly of mammalian prereplication complexes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 3, pp. 1347-52, 2002 (PubMed).

Shreeram, Sparks, Lane et al.: "Cell type-specific responses of human cells to inhibition of replication licensing." in: Oncogene, Vol. 21, Issue 43, pp. 6624-32, 2002 (PubMed).

Alternatives for antigen "Minichromosome Maintenance Complex Component 2 (MCM2)", type "Antibodies"
Hosts (100), (35), (3)
Reactivities (127), (45), (31), (13), (12), (12), (3), (3), (2), (1), (1)
Applications (108), (59), (33), (21), (18), (12), (10), (10), (9), (5), (4), (2), (2), (2), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (10), (9), (6), (5), (5), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
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