Arginase, Liver (ARG1) (AA 53-207) antibody
|Synonyms||AI, PGIF, Arg-1, AI256583, ARG1, arg1, MGC89217, MGC128755, zC146F4.3, SI:zC146F4.4 (novel protein with NUDIX domain), argi1|
Alternatives Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
|5 references available|
|Price||Product not available in this region.|
|Alternative name||Arginase I|
|Immunogen||Human Arginase I|
|Cross-Reactivity||Mouse (Murine), Rat (Rattus), Fruit Fly (Drosophila melanogaster)|
|Description||Arginase converts arginine into urea plus ornithine, the final step in urea synthesis. Two different isoforms (I&II) have been isolated with approximately 60% homology at the nucleotide level. While type II is present in many tissues, Arginase I is expressed exclusively in liver. In cultured macrophages, as well as in vivo, Arginase I is induced with nitric oxide synthase (NOS) and the arginase I transactivator C/EBPbeta in response to lipopolysaccharide. This response occurs in a dose and time-dependent manner. While the mRNA for NOS appears as early as 2h after treatment, mRNA levels for arginase I peak after twelve hours of lipopolysaccharide treatment. Since the synthesis of nitric oxide by NOS requires arginine, the delayed induction of arginase I may be necessary for the regulation of NOS activity. This antibody is routinely tested by western blot analysis.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||35 kDa|
Related Products: ABIN968543, ABIN967389
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20°C.|
|Restrictions||For Research Use only|
|Western blot analysis of Arginase I on a mouse liver lysate. Lane 1: 1:1000, lane 2: 1: 2000, lane 3: 1:4000 dilution of the mouse anti-arginase 1 antibody. Immunofluorescence staining of mouse macrophages.|
Haraguchi, Takiguchi, Amaya et al.: "Molecular cloning and nucleotide sequence of cDNA for human liver arginase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 2, pp. 412-5, 1987 (PubMed).
Dizikes, Grody, Kern et al.: "Isolation of human liver arginase cDNA and demonstration of nonhomology between the two human arginase genes." in: Biochemical and biophysical research communications, Vol. 141, Issue 1, pp. 53-9, 1987 (PubMed).
Sonoki, Nagasaki, Gotoh et al.: "Coinduction of nitric-oxide synthase and arginase I in cultured rat peritoneal macrophages and rat tissues in vivo by lipopolysaccharide." in: The Journal of biological chemistry, Vol. 272, Issue 6, pp. 3689-93, 1997 (PubMed).
Chang, Zoghi, Liao et al.: "The involvement of tyrosine kinases, cyclic AMP/protein kinase A, and p38 mitogen-activated protein kinase in IL-13-mediated arginase I induction in macrophages: its implications in IL-13-inhibited nitric oxide production." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 4, pp. 2134-41, 2000 (PubMed).
Morrison, Correll: "Activation of the stem cell-derived tyrosine kinase/RON receptor tyrosine kinase by macrophage-stimulating protein results in the induction of arginase activity in murine peritoneal macrophages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 2, pp. 853-60, 2002 (PubMed).
|Hosts||Rabbit (50), Goat (4), Mouse (2)|
|Reactivities||Human (45), Mouse (Murine) (28), Rat (Rattus) (27), Cow (Bovine) (14), Rabbit (6), Dog (Canine) (5), Pig (Porcine) (3), Cat (Feline) (1), Chicken (1), Xenopus laevis (1)|
|Applications||Western Blotting (WB) (38), ELISA (27), Immunofluorescence (IF) (17), Immunohistochemistry (IHC) (13), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (7), Immunoprecipitation (IP) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Flow Cytometry (FACS) (2), Dot Blot (Dot) (1), Immunocytochemistry (ICC) (1), Immunodiffusion (ID) (1), Immunoelectron Microscopy (IEM) (1), Immunoelectrophoresis (IEP) (1), Radioimmunoassay (RIA) (1)|
|Conjugates||Biotin (2), HRP (2), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), PE (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1)|
|Epitopes||C-Term (3), N-Term (3), AA 292-318 (1), AA 293-322 (1)|