Centromere Protein F (CENPF) (AA 209-381) antibody

Details for Product No. ABIN968167
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms LOC100222985, 6530404A22Rik, AI325968, CENF, Lek1, PRO1779, mitosin, im:7140452, im:7144238, im:7154719, im:7156761, si:ch211-152h14.2, hcp-1, CMF1
Epitope
AA 209-381
(9), (5), (2), (2), (1), (1)
Reactivity
Human
(42), (1), (1), (1)
Host
Mouse
(27), (11), (6)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF)
(20), (17), (16), (11), (9), (6), (5), (5), (2), (2), (1)
Pubmed 5 references available
Quantity 50 μg
Options
Shipping to United States (Change)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN968167
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Human Mitosin
Clone 4H3
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Mitosin
Background Mitosin, a nuclear proetin of 3113 amino acids, contains a pair of tandem repeats and seven leucine repeats. In vitro, Mitosin directly interacts with the retinoblastoma protein, Rb. Mitosin is expressed throughout the cell cycle, with levels being lowest during G1, and it localizes at the kinetochore during the mitotic phase. The subcellular redistribution of Mitosin to the kinetochore is linked to its phosphorylation. Its expression is linked to patients with autoimmune diseases characterized by abnormal cell proliferation. Ectopic expression of a truncated version of Mitosin blocks the progression of the cell cycle. This suggests that the protein has an important role during cell proliferation. Because of similarities in subcellular localization and expression, Mitosin and the autoantigen p300/CENP-F, which is also linked to a multitude of autoimmune disorders, are probably the same protein.
Molecular Weight 357 kDa
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Centromere Protein F (CENPF) (AA 209-381) antibody Immunofluorescence staining of human endothelial cells.
anti-Centromere Protein F (CENPF) (AA 209-381) antibody (2) Western blot analysis of Mitosin on a Jurkat cell lysate (Human T-cell leukemia, ATCC TIB-152). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti-human Mitosin antibody.
anti-Centromere Protein F (CENPF) (AA 209-381) antibody (3) anti-Centromere Protein F (CENPF) (AA 209-381) antibody (Image 3)
Product cited in: Casiano, Humbel, Peebles et al.: "Autoimmunity to the cell cycle-dependent centromere protein p330d/CENP-F in disorders associated with cell proliferation." in: Journal of autoimmunity, Vol. 8, Issue 4, pp. 575-86, 1996 (PubMed).

Zhu, Mancini, Chang et al.: "Characterization of a novel 350-kilodalton nuclear phosphoprotein that is specifically involved in mitotic-phase progression." in: Molecular and cellular biology, Vol. 15, Issue 9, pp. 5017-29, 1995 (PubMed).

Rattner, Rao, Fritzler et al.: "CENP-F is a .ca 400 kDa kinetochore protein that exhibits a cell-cycle dependent localization." in: Cell motility and the cytoskeleton, Vol. 26, Issue 3, pp. 214-26, 1994 (PubMed).

Landberg, Erlanson, Roos et al.: "Nuclear autoantigen p330d/CENP-F: a marker for cell proliferation in human malignancies." in: Cytometry, Vol. 25, Issue 1, pp. 90-8, 1997 (PubMed).

Trinkle-Mulcahy, Andrews, Wickramasinghe et al.: "Time-lapse imaging reveals dynamic relocalization of PP1gamma throughout the mammalian cell cycle." in: Molecular biology of the cell, Vol. 14, Issue 1, pp. 107-17, 2003 (PubMed).

Validation Images
Did you look for something else?
back to top