anti-CENPF antibody (Centromere Protein F) (AA 209-381)

Details for Product anti-CENPF Antibody No. ABIN968167
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Synonyms LOC100222985, 6530404A22Rik, AI325968, CENF, Lek1, PRO1779, mitosin, im:7140452, im:7144238, im:7154719, im:7156761, si:ch211-152h14.2, hcp-1, CMF1
AA 209-381
(21), (8), (2), (2), (2), (1), (1)
(65), (3), (3), (3)
(32), (18), (16)
Clonality (Clone)
Monoclonal ()
This CENPF antibody is un-conjugated
(3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
(25), (22), (17), (17), (15), (11), (10), (7), (5), (3), (1), (1)
Pubmed 5 references available
Quantity 50 μg
Shipping to United States ( )
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Immunogen Human Mitosin aa. 209-381
Clone 11-Mitosin
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Mitosin (CENPF Antibody Abstract)
Background Mitosin, a nuclear proetin of 3113 amino acids, contains a pair of tandem repeats and seven leucine repeats. In vitro, Mitosin directly interacts with the retinoblastoma protein, Rb. Mitosin is expressed throughout the cell cycle, with levels being lowest during G1, and it localizes at the kinetochore during the mitotic phase. The subcellular redistribution of Mitosin to the kinetochore is linked to its phosphorylation. Its expression is linked to patients with autoimmune diseases characterized by abnormal cell proliferation. Ectopic expression of a truncated version of Mitosin blocks the progression of the cell cycle. This suggests that the protein has an important role during cell proliferation. Because of similarities in subcellular localization and expression, Mitosin and the autoantigen p300/CENP-F, which is also linked to a multitude of autoimmune disorders, are probably the same protein.
Molecular Weight 357 kDa

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-CENPF antibody (Centromere Protein F) (AA 209-381) (ABIN968167) Western blot analysis of Mitosin on a Jurkat cell lysate (Human T-cell leukemia, ATCC...
Immunofluorescence (IF) image for anti-CENPF antibody (Centromere Protein F) (AA 209-381) (ABIN968167) Immunofluorescence staining of human endothelial cells.
Image no. 3 for anti-CENPF antibody (Centromere Protein F) (AA 209-381) (ABIN968167) anti-Centromere Protein F (CENPF) (AA 209-381) antibody (Image 3)
Product cited in: Trinkle-Mulcahy, Andrews, Wickramasinghe et al.: "Time-lapse imaging reveals dynamic relocalization of PP1gamma throughout the mammalian cell cycle." in: Molecular biology of the cell, Vol. 14, Issue 1, pp. 107-17, 2003 (PubMed).

Landberg, Erlanson, Roos et al.: "Nuclear autoantigen p330d/CENP-F: a marker for cell proliferation in human malignancies." in: Cytometry, Vol. 25, Issue 1, pp. 90-8, 1997 (PubMed).

Casiano, Humbel, Peebles et al.: "Autoimmunity to the cell cycle-dependent centromere protein p330d/CENP-F in disorders associated with cell proliferation." in: Journal of autoimmunity, Vol. 8, Issue 4, pp. 575-86, 1996 (PubMed).

Background publications Rattner, Rao, Fritzler et al.: "CENP-F is a .ca 400 kDa kinetochore protein that exhibits a cell-cycle dependent localization." in: Cell motility and the cytoskeleton, Vol. 26, Issue 3, pp. 214-26, 1994 (PubMed).

Zhu, Mancini, Chang et al.: "Characterization of a novel 350-kilodalton nuclear phosphoprotein that is specifically involved in mitotic-phase progression." in: Molecular and cellular biology, Vol. 15, Issue 9, pp. 5017-29, 1995 (PubMed).

Validation Images
Catalog No. ABIN968167
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