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N-Cadherin antibody (Cadherin 2) (AA 802-819)

Details for Product anti-CDH2 Antibody No. ABIN968249, Supplier: Login to see
Antigen
  • N-cadherin
  • cadherin-2
  • cdhn
  • ncad
  • cdw325
  • CDHN
  • Ncad
  • glo
  • lyr
  • pac
  • wu:fb47h04
  • CD325
  • CDw325
  • NCAD
  • CDH2
  • Cadherin-2
Epitope
AA 802-819
19
13
13
11
11
10
9
7
7
6
6
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Human, Mouse (Murine), Rat (Rattus)
214
88
78
23
9
5
4
4
2
2
2
1
1
1
1
1
1
1
1
1
Host
Mouse
134
99
6
2
2
Clonality (Clone)
Monoclonal ()
Conjugate
This N-Cadherin antibody is un-conjugated
13
8
7
6
4
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
160
58
48
47
42
35
34
22
13
12
3
2
2
2
1
1
1
1
Supplier
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Immunogen Mouse N-Cadherin aa. 802-819
Clone 32-N
Isotype IgG1
Cross-Reactivity Human, Rat (Rattus), Chicken
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name N-Cadherin (CDH2 Antibody Abstract)
Background Cadherins are a family of Ca2+-dependent intercellular adhesion molecules that play a central role in controlling morphogenetic movements during development. Their function is regulated by association with the actin cytoskeleton by a complex of cytoplasmic proteins called the catenins (alpha, beta, gamma). Members of the cadherin family include P-cadherin, E-cadherin (uvomorulin), N-cadherin (neural cadherin), R-cadherin, cadherin 5, L-CAM, and EP-cadherin. N-cadherin mRNA is found at elevated levels in brain and heart and at a much lower level in liver. Mechanisms such as mRNA expression, cytokine modulation, and protease-mediated turnover modulate N-cadherin protein levels during development. In addition, N-cadherin function is indirectly regulated by endogenous kinases and phosphatases. Tyrosine phosphorylation of beta-catenin complexed with N-cadherin results in dissociation of N-cadherin from actin. However, N-cadherin also interacts with a PTP1B-like phosphatase that dephosphorylates beta-catenin and promotes N-cadherin/actin association. Thus, N-cadherin is an integral adhesion molecule whose function is regulated by protein-protein interactions and phosphorylation/dephosphorylation events.
Molecular Weight 130 kDa
Pathways
Comment

Related Products: ABIN967389, ABIN968535

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-N-Cadherin antibody (Cadherin 2) (AA 802-819) (ABIN968249) Western blot analysis of N-Cadherin on HeLa lysate. Lane 1: 1:250, lane 2: 1:500, lan...
Immunofluorescence (IF) image for anti-N-Cadherin antibody (Cadherin 2) (AA 802-819) (ABIN968249) Immunofluorescent staining of 293 cells with anti- N-cadherin antibody.
Product cited in: Nürnberger, Bacallao, Phillips: "Inversin forms a complex with catenins and N-cadherin in polarized epithelial cells." in: Molecular biology of the cell, Vol. 13, Issue 9, pp. 3096-106, 2002 (PubMed).

Izawa, Nishizawa, Ohtakara et al.: "Densin-180 interacts with delta-catenin/neural plakophilin-related armadillo repeat protein at synapses." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 5345-50, 2002 (PubMed).

Miyatani, Shimamura, Hatta et al.: "Neural cadherin: role in selective cell-cell adhesion." in: Science (New York, N.Y.), Vol. 245, Issue 4918, pp. 631-5, 1989 (PubMed).

Background publications Cowin: "Unraveling the cytoplasmic interactions of the cadherin superfamily." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 23, pp. 10759-61, 1994 (PubMed).