N-Cadherin antibody (AA 690-770)

Catalog No. ABIN7215970

The Rabbit Polyclonal anti-N-Cadherin antibody is suitable to detect N-Cadherin in samples from Human, Mouse and Rat. It has been validated for WB, IHC, IF and ELISA.

Quick Overview for N-Cadherin antibody (AA 690-770) (ABIN7215970)

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This N-Cadherin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Product Details anti-N-Cadherin Antibody

Binding Specificity: AA 690-770

Purpose: Rabbit Anti-N-cadherin Polyclonal Antibody

Specificity: N-cadherin Polyclonal Antibody detects endogenous levels of N-cadherin protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human N-cadherin at AA range: 690-770

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for N-Cadherin

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Alternative Name: N-cadherin

Background: CDH2, CDHN, NCAD, Cadherin-2, CDw325, Neural cadherin, N-cadherin, CD antigen CD325CDH2 encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. Cadherin-2 plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone.

Molecular Weight: 100-140kD

Gene ID: 1000

UniProt: P19022

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane