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TJP1 antibody (AA 1048-1247)

TJP1 Reactivity: Human WB, IF Host: Mouse Monoclonal 1-ZO unconjugated
Catalog No. ABIN968282
  • Target See all TJP1 Antibodies
    TJP1 (Tight Junction Protein 1 (TJP1))
    Binding Specificity
    • 16
    • 6
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1048-1247
    Reactivity
    • 53
    • 32
    • 30
    • 20
    • 18
    • 9
    • 6
    • 5
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 49
    • 3
    • 2
    • 1
    • 1
    Mouse
    Clonality
    • 51
    • 5
    Monoclonal
    Conjugate
    • 38
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This TJP1 antibody is un-conjugated
    Application
    • 51
    • 20
    • 18
    • 15
    • 14
    • 13
    • 12
    • 11
    • 11
    • 8
    • 7
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunofluorescence (IF)
    Characteristics
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Human ZO-1 aa. 1048-1247
    Clone
    1-ZO
    Isotype
    IgG1
    Top Product
    Discover our top product TJP1 Primary Antibody
  • Comment

    Related Products: ABIN968535, ABIN967389

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store undiluted at -20° C.
  • Hirase, Kawashima, Wong, Ueyama, Rikitake, Tsukita, Yokoyama, Staddon: "Regulation of tight junction permeability and occludin phosphorylation by Rhoa-p160ROCK-dependent and -independent mechanisms." in: The Journal of biological chemistry, Vol. 276, Issue 13, pp. 10423-31, (2001) (PubMed).

    Nix, Chishti, Anderson, Walther: "hCASK and hDlg associate in epithelia, and their src homology 3 and guanylate kinase domains participate in both intramolecular and intermolecular interactions." in: The Journal of biological chemistry, Vol. 275, Issue 52, pp. 41192-200, (2001) (PubMed).

    Ebnet, Schulz, Meyer Zu Brickwedde, Pendl, Vestweber: "Junctional adhesion molecule interacts with the PDZ domain-containing proteins AF-6 and ZO-1." in: The Journal of biological chemistry, Vol. 275, Issue 36, pp. 27979-88, (2000) (PubMed).

    Willott, Balda, Fanning, Jameson, Van Itallie, Anderson: "The tight junction protein ZO-1 is homologous to the Drosophila discs-large tumor suppressor protein of septate junctions." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 16, pp. 7834-8, (1993) (PubMed).

  • Target
    TJP1 (Tight Junction Protein 1 (TJP1))
    Alternative Name
    ZO-1 (TJP1 Products)
    Synonyms
    ZO-1 antibody, ZO1 antibody, zo1 antibody, tight junction protein 1 antibody, tight junction protein ZO-1 antibody, TJP1 antibody, Tjp1 antibody, LOC396567 antibody
    Background
    Tight junctions (zonulae occludens) are critical to the maintenance of cell polarity and intracellular barriers between epithelial and endothelial cells. Protein components of the tight junctions include actin filaments, symplekin, occludin, Rab3B, AF-6, 7H6, ZO-1, and ZO-2. Analysis of ZO-1 and -2 resulted in their inclusion in the MAGUK protein family. This family also includes the discs large tumor suppressor protein (Dlg-A) of Drosophila, p55, an erythrocyte membrane protein, and PSD-95/SAP90, a synaptic membrane protein. All family members contain a region homologous to guanylate kinase (GuK), a src homology (SH3) domain, and multiple PDZ domains. Through these elements, MAGUK proteins function in signal transduction and, possibly, tumor suppression. ZO-1 is a peripheral membrane phosphoprotein that binds to other tight junction proteins such as occludin and AF-6. Via its SH3 domain, ZO-1 interacts with a serine protein kinase that phosphorylates a region immediately C-terminal of the SH3 domain. Taken together, these data indicate that ZO-1 is a critical element in the formation of tight junctions and may also serve an important role in signaling and tumor suppression. This antibody is routinely tested by western blot analysis.
    Molecular Weight
    220 kDa
    Pathways
    Carbohydrate Homeostasis, Cell-Cell Junction Organization
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