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Tight Junction Protein 1 (Zona Occludens 1) (TJP1) (AA 1048-1247) antibody

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Synonyms ZO-1, ZO1, zo1
AA 1048-1247
(13), (11), (6), (5), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
(80), (26), (25), (13), (7), (1), (1), (1), (1)
(65), (15), (4), (2), (1)
Clonality (Clone)
Monoclonal ()
(3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Immunofluorescence (IF), Western Blotting (WB)
(66), (22), (20), (17), (15), (12), (11), (9), (8), (7), (4), (1), (1), (1)
Pubmed 4 references available
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Quantity 50 μg
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Immunogen Human ZO-1 aa. 1048-1247
Clone 1-ZO
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name ZO-1 (TJP1 Antibody Abstract)
Background Tight junctions (zonulae occludens) are critical to the maintenance of cell polarity and intracellular barriers between epithelial and endothelial cells. Protein components of the tight junctions include actin filaments, symplekin, occludin, Rab3B, AF-6, 7H6, ZO-1, and ZO-2. Analysis of ZO-1 and -2 resulted in their inclusion in the MAGUK protein family. This family also includes the discs large tumor suppressor protein (Dlg-A) of Drosophila, p55, an erythrocyte membrane protein, and PSD-95/SAP90, a synaptic membrane protein. All family members contain a region homologous to guanylate kinase (GuK), a src homology (SH3) domain, and multiple PDZ domains. Through these elements, MAGUK proteins function in signal transduction and, possibly, tumor suppression. ZO-1 is a peripheral membrane phosphoprotein that binds to other tight junction proteins such as occludin and AF-6. Via its SH3 domain, ZO-1 interacts with a serine protein kinase that phosphorylates a region immediately C-terminal of the SH3 domain. Taken together, these data indicate that ZO-1 is a critical element in the formation of tight junctions and may also serve an important role in signaling and tumor suppression. This antibody is routinely tested by western blot analysis.
Molecular Weight 220 kDa

Related Products: ABIN968535, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-Tight Junction Protein 1 (Zona Occludens 1) (TJP1) (AA 1048-1247) antibody (ABIN968282) Western blot analysis of ZO-1 on a HeLa cell lysate. Lane 1: 1:250, lane 2: 1:500, la...
Immunofluorescence (IF) image for anti-Tight Junction Protein 1 (Zona Occludens 1) (TJP1) (AA 1048-1247) antibody (ABIN968282) Immunofluorescent staining of EAHY human endothelial cells.
Product cited in: Hirase, Kawashima, Wong et al.: "Regulation of tight junction permeability and occludin phosphorylation by Rhoa-p160ROCK-dependent and -independent mechanisms." in: The Journal of biological chemistry, Vol. 276, Issue 13, pp. 10423-31, 2001 (PubMed).

Nix, Chishti, Anderson et al.: "hCASK and hDlg associate in epithelia, and their src homology 3 and guanylate kinase domains participate in both intramolecular and intermolecular interactions." in: The Journal of biological chemistry, Vol. 275, Issue 52, pp. 41192-200, 2001 (PubMed).

Ebnet, Schulz, Meyer Zu Brickwedde et al.: "Junctional adhesion molecule interacts with the PDZ domain-containing proteins AF-6 and ZO-1." in: The Journal of biological chemistry, Vol. 275, Issue 36, pp. 27979-88, 2000 (PubMed).

Willott, Balda, Fanning et al.: "The tight junction protein ZO-1 is homologous to the Drosophila discs-large tumor suppressor protein of septate junctions." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 16, pp. 7834-8, 1993 (PubMed).

Catalog No. ABIN968282
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