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BiP (AA 525-628) antibody

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Antigen
Synonyms Bip, Sez7, mBiP, Grp78, SEZ-7, Hsce70, AL022860, AU019543, D2Wsu17e, D2Wsu141e
Epitope
»Alternatives AA 525-628
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ( )
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB)
Pubmed 5 references available
Catalog no. ABIN968290
Quantity 50 µg  (Variants)
Price
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anti-BiP (AA 525-628) antibody

Product details

Target Details Application Details Handling Images References Alternatives Variants back to top
Immunogen Human BiP/GRP78
Clone
Isotype IgG2a
Cross-Reactivity Dog (Canine), Rat (Rattus), Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified

Target Details

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Background Synthesis of nascent proteins occurs at sites on the endoplasmic reticulum (ER) called translocons. Translocon proteins form a pore in the membrane that allow passage of the newly synthesized protein from the ribosome into the ER lumen. As the nascent protein enters the lumen, it is bound by BiP (binding protein), the major chaperone of the ER. This protein is identical to the 78kDa glucose regulated protein, GRP78. BiP binds short hydrophobic sequences of the emerging peptide and prevents denaturation or nonspecific aggregation. Hydrolysis of ATP by BiP results in the release of the nascent protein which quickly assumes its proper conformation. However, if folding is incorrect, BiP again binds the protein and prevents its exit from the ER. In addition, BiP binding is thought to enhance the movement of secretory polypeptides across the ER membrane, but it is not required for protein translocation. It is 60% identical to Hsp70 and is similarly increased by conditions that produce incorrectly folded proteins. Thus, BiP is a chaperone of the ER lumen that binds misfolded or unassembled secretory proteins and ensures proper movement of proteins from the ER to the Golgi apparatus.
Molecular Weight 78 kDa

Application Details

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Comment

Related Products: ABIN968587, ABIN967389
Restrictions For Research Use only

Handling

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Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative 0.09% Sodium azide.
Storage -20 °C

References

Target Details Application Details Handling Images Alternatives Variants back to top
Product cited in: Ting, Lee: "Human gene encoding the 78,000-dalton glucose-regulated protein and its pseudogene: structure, conservation, and regulation." in: DNA (Mary Ann Liebert, Inc.), Vol. 7, Issue 4, pp. 275-86, 1988 (PubMed).

Yamazaki, Chang, Haass et al.: "Accumulation and aggregation of amyloid beta-protein in late endosomes of Niemann-pick type C cells." in: The Journal of biological chemistry, Vol. 276, Issue 6, pp. 4454-60, 2001 (PubMed).

Waelter, Boeddrich, Lurz et al.: "Accumulation of mutant huntingtin fragments in aggresome-like inclusion bodies as a result of insufficient protein degradation." in: Molecular biology of the cell, Vol. 12, Issue 5, pp. 1393-407, 2001 (PubMed).

Morishima, Nakanishi, Takenouchi et al.: "An endoplasmic reticulum stress-specific caspase cascade in apoptosis. Cytochrome c-independent activation of caspase-9 by caspase-12." in: The Journal of biological chemistry, Vol. 277, Issue 37, pp. 34287-94, 2002 (PubMed).

Han, Kim, Lee et al.: "Localization of phospholipase D1 to caveolin-enriched membrane via palmitoylation: implications for epidermal growth factor signaling." in: Molecular biology of the cell, Vol. 13, Issue 11, pp. 3976-88, 2002 (PubMed).

Alternatives

Target Details Application Details Handling Images References Variants back to top
Alternatives for antigen "BiP", type "Antibodies"
Hosts (5), (2)
Reactivities (7), (5), (5)
Applications (7), (4), (1), (1), (1)
Conjugates (1)
Epitopes (1)
Beacon
Guglhupf
Guglhupf Guglhupf Guglhupf