Microtubule-Associated Protein 4 (MAP4) (AA 583-702) antibody

Antigen: Microtubule-Associated Protein 4 (MAP4)

Synonyms: MGC8617, DKFZp779A1753, MAP4, Mtap-4, AA407148, Mtap4, XMAP4, mtap4

Binding Site: AA 583-702

Clonality: Monoclonal (18)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968326

Additional Information

Alternative name: MAP4

Immunogen: Human MAP4

Format: Liquid

Isotype: IgG1

Clone: 18

Description: The microtubule (MT) cytoskeleton functions in cytoplasmic organization, cellular movement, determination of cell polarity, intracellular transport, and chromosome segregation. Dynamic instability, continuous cycles of MT assembly (stabilization) and disassembly, mediates MT participation in these events. The function of MTs, particularly stabilization, is regulated by MT-associated proteins (MAPs). A subfamily of MAPs, called AP-MAPs (assembly promoting MAPs), includes tau, MAP2, and MAP4. These proteins are classified as type II MAPs and contain a C-terminal MT binding domain with 3 to 5 imperfect repeats of an 18 amino acid motif. While tau and MAP2 are specifically expressed in neuronal cells, MAP4 is the major MAP of nonneuronal mammalian cells. MAP activity and interaction with MTs are regulated by MARK (MAP/MT affinity-regulating kinase) and mapmodulin. MARK-mediated phosphorylation of MAPs on their homologous KXGS motifs results in detachment of MAPs from MTs and MT disruption. In addition, mapmodulin tightly interacts with the MT-binding domains of MAPs and hinders MAP binding to MTs. Thus, MAP4 is a highly regulated type II MAP that controls MT assembly and disassembly.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 200-220 kDa

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of MAP4 on HepG2 lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1: 4000 dilution of MAP4 .

Human Endothelial

anti-Microtubule-Associated Protein 4 (MAP4) (AA 583-702) antibody (Image 3)

Publications

Product: Chapin, Lue, Yu et al.: "Differential expression of alternatively spliced forms of MAP4: a repertoire of structurally different microtubule-binding domains." in: Biochemistry, Vol. 34, Issue 7, pp. 2289-301, 1995 (PubMed).

Drewes, Ebneth, Preuss et al.: "MARK, a novel family of protein kinases that phosphorylate microtubule-associated proteins and trigger microtubule disruption." in: Cell, Vol. 89, Issue 2, pp. 297-308, 1997 (PubMed).

Ulitzur, Humbert, Pfeffer: "Mapmodulin: a possible modulator of the interaction of microtubule-associated proteins with microtubules." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 10, pp. 5084-9, 1997 (PubMed).