MAP4 antibody (AA 583-702)

Catalog No. ABIN968326

Product Details anti-MAP4 Antibody

Target: MAP4 (Microtubule-Associated Protein 4 (MAP4))

Binding Specificity: AA 583-702

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAP4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human MAP4 aa. 583-702

Clone: 18-MAP4

Isotype: IgG1

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-MAP4 antibody (ABIN968326)

Drewes, Ebneth, Preuss, Mandelkow, Mandelkow: "MARK, a novel family of protein kinases that phosphorylate microtubule-associated proteins and trigger microtubule disruption." in: Cell, Vol. 89, Issue 2, pp. 297-308, (1997) (PubMed).

Ulitzur, Humbert, Pfeffer: "Mapmodulin: a possible modulator of the interaction of microtubule-associated proteins with microtubules." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 10, pp. 5084-9, (1997) (PubMed).

Chapin, Lue, Yu, Bulinski: "Differential expression of alternatively spliced forms of MAP4: a repertoire of structurally different microtubule-binding domains." in: Biochemistry, Vol. 34, Issue 7, pp. 2289-301, (1995) (PubMed).

Target Details for MAP4

Target: MAP4 (Microtubule-Associated Protein 4 (MAP4))

Alternative Name: MAP4 (MAP4 Products)

Synonyms: XMAP4 antibody, mtap4 antibody, AA407148 antibody, MAP-4 antibody, Mtap-4 antibody, Mtap4 antibody, microtubule associated protein 4 antibody, microtubule associated protein 4 L homeolog antibody, microtubule-associated protein 4 antibody, MAP4 antibody, map4.L antibody, Map4 antibody

Background: The microtubule (MT) cytoskeleton functions in cytoplasmic organization, cellular movement, determination of cell polarity, intracellular transport, and chromosome segregation. Dynamic instability, continuous cycles of MT assembly (stabilization) and disassembly, mediates MT participation in these events. The function of MTs, particularly stabilization, is regulated by MT-associated proteins (MAPs). A subfamily of MAPs, called AP-MAPs (assembly promoting MAPs), includes tau, MAP2, and MAP4. These proteins are classified as type II MAPs and contain a C-terminal MT binding domain with 3 to 5 imperfect repeats of an 18 amino acid motif. While tau and MAP2 are specifically expressed in neuronal cells, MAP4 is the major MAP of nonneuronal mammalian cells. MAP activity and interaction with MTs are regulated by MARK (MAP/MT affinity-regulating kinase) and mapmodulin. MARK-mediated phosphorylation of MAPs on their homologous KXGS motifs results in detachment of MAPs from MTs and MT disruption. In addition, mapmodulin tightly interacts with the MT-binding domains of MAPs and hinders MAP binding to MTs. Thus, MAP4 is a highly regulated type II MAP that controls MT assembly and disassembly.

Molecular Weight: 200-220 kDa

Pathways: p53 Signaling, Microtubule Dynamics