M-Cadherin (AA 253-366) antibody

Details for Product No. ABIN968364
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Antigen
Synonyms AI323380, Cdh14, Mcad, CDH14, CDH3, CDHM, MCAD, MRD3
Epitope
AA 253-366
(9), (9), (6), (6), (1), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine)
(52), (24), (21), (12), (12)
Host
Mouse
(45), (8), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF)
(40), (37), (18), (10), (8), (7), (6), (6)
Pubmed 5 references available
Quantity 150 µg
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Catalog No. ABIN968364
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Immunogen Mouse M-Cadherin
Clone 5
Isotype IgG2a
Cross-Reactivity Rat (Rattus)
Characteristics 1. Please refer to us for technical protocols.
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Background Cadherins are a family of transmembrane glycoproteins involved in the Ca2+-dependent cell-cell adhesion that occurs in many tissues. Members of this family include P-Cadherin, E-Cadherin (uvomorulin), N-Cadherin, R-Cadherin, Cadherin-5, L-CAM, and EP-Cadherin. These proteins are similar in their domain structure (45-74% amino acid conservation), Ca2+ and protease sensitivity, and molecular weight. However, cadherins have distinct tissue expression patterns and immunological reactivities. M (muscle)-Cadherin, another member of the Cadherin family, was discovered in myogenic mouse cells where it is present at low levels in myoblasts. It is expressed in prenatal and adult skeletal muscle and plays a role in skeletal muscle cell differentiation, particularly the fusion of myoblasts into myotubes. It is upregulated upon induction of myotube formation. M-Cadherin also forms complexes with the catenins in skeletal muscle cells, which then interact with the cytoskeleton. Therefore, it is thought that the M-Cadherin-cytoskeleton interaction may play a role in aligning myoblasts during fusion.
Molecular Weight 130 kDa
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-M-Cadherin (AA 253-366) antibody Western blot analysis of M-Cadherin on mouse neonate lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-M-Cadherin.
anti-M-Cadherin (AA 253-366) antibody (2) anti-M-Cadherin (AA 253-366) antibody (Image 2)
Product cited in: Donalies, Cramer, Ringwald et al.: "Expression of M-cadherin, a member of the cadherin multigene family, correlates with differentiation of skeletal muscle cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 18, pp. 8024-8, 1991 (PubMed).

Kuch, Winnekendonk, Butz et al.: "M-cadherin-mediated cell adhesion and complex formation with the catenins in myogenic mouse cells." in: Experimental cell research, Vol. 232, Issue 2, pp. 331-8, 1997 (PubMed).

Shimoyama, Shibata, Kitajima et al.: "Molecular cloning and characterization of a novel human classic cadherin homologous with mouse muscle cadherin." in: The Journal of biological chemistry, Vol. 273, Issue 16, pp. 10011-8, 1998 (PubMed).

Kaufmann, Kirsch, Irintchev et al.: "The M-cadherin catenin complex interacts with microtubules in skeletal muscle cells: implications for the fusion of myoblasts." in: Journal of cell science, Vol. 112 ( Pt 1), pp. 55-68, 1999 (PubMed).

Kang, Feinleib, Knox et al.: "Promyogenic members of the Ig and cadherin families associate to positively regulate differentiation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 7, pp. 3989-94, 2003 (PubMed).

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