TGFB1I1 antibody (AA 73-186)

Catalog No. ABIN968401

Product Details anti-TGFB1I1 Antibody

Target: TGFB1I1 (Transforming Growth Factor beta 1 Induced Transcript 1 (TGFB1I1))

Binding Specificity: AA 73-186

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This TGFB1I1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Cross-Reactivity: Rat (Rattus), Human, Dog (Canine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Hic-5 aa. 73-186

Clone: 34-Hic

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-TGFB1I1 antibody (ABIN968401)

Wang, Yang, Guo, Sampson, Hsu, Tsai, Yeh, Wu, Guo, Chang: "Suppression of androgen receptor transactivation by Pyk2 via interaction and phosphorylation of the ARA55 coregulator." in: The Journal of biological chemistry, Vol. 277, Issue 18, pp. 15426-31, (2002) (PubMed).

Jia, Ransom, Shibanuma, Liu, Welsh, Smoyer: "Identification and characterization of hic-5/ARA55 as an hsp27 binding protein." in: The Journal of biological chemistry, Vol. 276, Issue 43, pp. 39911-8, (2001) (PubMed).

Matsuya, Sasaki, Aoto, Mitaka, Nagura, Ohba, Ishino, Takahashi, Suzuki, Sasaki: "Cell adhesion kinase beta forms a complex with a new member, Hic-5, of proteins localized at focal adhesions." in: The Journal of biological chemistry, Vol. 273, Issue 2, pp. 1003-14, (1998) (PubMed).

Shibanuma, Mashimo, Kuroki, Nose: "Characterization of the TGF beta 1-inducible hic-5 gene that encodes a putative novel zinc finger protein and its possible involvement in cellular senescence." in: The Journal of biological chemistry, Vol. 269, Issue 43, pp. 26767-74, (1994) (PubMed).

Target Details for TGFB1I1

Target: TGFB1I1 (Transforming Growth Factor beta 1 Induced Transcript 1 (TGFB1I1))

Alternative Name: Hic-5 (TGFB1I1 Products)

Synonyms: TGFB1I1 antibody, hic5 antibody, ara55 antibody, hic-5 antibody, tsc-5 antibody, ARA55 antibody, HIC-5 antibody, HIC5 antibody, TSC-5 antibody, Hic5 antibody, Ara55 antibody, Hic-5 antibody, ara-55 antibody, transforming growth factor beta 1 induced transcript 1 antibody, transforming growth factor beta 1 induced transcript 1 L homeolog antibody, TGFB1I1 antibody, tgfb1i1 antibody, Tgfb1i1 antibody, tgfb1i1.L antibody

Background: Focal adhesions are cell structures terminal to the actin-stress fiber bundles. They attach cultured cells to their substratum or extracellular matrix. These adhesions occur through the integrin receptor molecules which link cytoskeletal proteins and extracellular matrix. The intracellular adhesion is composed of a number of proteins such as paxillin, VASP, vinculin, and the focal-adhesion kinases FAK and PYK2/CAKbeta. Hic-5, also described as hydrogen peroxide inducible-mRNA, is a focal adhesion protein that binds to FAK and PYK2. It is ubiquitously expressed with the highest levels found in lung, spleen, and heart. Induction of Hic-5 is accomplished by hydrogen peroxide or TGFbeta1 and is repressed in K-ras transformed cells. Like paxillin, Hic-5 is tyrosine phosphorylated in Src-transformed cells and is highly similar to paxillin in its primary structure. Both proteins contain LIM domains and LD motifs. In addition, Hic-5 localizes to focal adhesions and co-immunoprecipitates with PYK2/CAKbeta in vivo. Thus, Hic-5 may be a substrate for CAKbeta and play a role in signal transduction during proliferation, cell motility, and adhesion.

Molecular Weight: 50 kDa

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, VEGF Signaling