Transforming Growth Factor beta 1 Induced Transcript 1 (TGFB1I1) (AA 73-186) antibody

Details for Product No. ABIN968401
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Synonyms HIC5, ARA55, HIC-5, TSC-5, Hic5, hic-5, Ara55, Hic-5, ara-55, ara55, hic5, tsc-5, TGFB1I1
AA 73-186
(4), (2), (1), (1), (1), (1), (1)
Mouse (Murine)
(34), (20), (20), (13), (12), (12)
(29), (5), (1)
Clonality (Clone)
Monoclonal ()
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB), Immunofluorescence (IF)
(19), (17), (10), (7), (6), (4), (3), (2), (2), (2), (2), (1)
Pubmed 4 references available
Catalog no. ABIN968401
Quantity 150 µg
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Immunogen Mouse Hic-5
Clone L10CC10
Isotype IgG1
Cross-Reactivity Rat (Rattus), Human, Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name Hic-5
Background Focal adhesions are cell structures terminal to the actin-stress fiber bundles. They attach cultured cells to their substratum or extracellular matrix. These adhesions occur through the integrin receptor molecules which link cytoskeletal proteins and extracellular matrix. The intracellular adhesion is composed of a number of proteins such as paxillin, VASP, vinculin, and the focal-adhesion kinases FAK and PYK2/CAKbeta. Hic-5, also described as hydrogen peroxide inducible-mRNA, is a focal adhesion protein that binds to FAK and PYK2. It is ubiquitously expressed with the highest levels found in lung, spleen, and heart. Induction of Hic-5 is accomplished by hydrogen peroxide or TGFbeta1 and is repressed in K-ras transformed cells. Like paxillin, Hic-5 is tyrosine phosphorylated in Src-transformed cells and is highly similar to paxillin in its primary structure. Both proteins contain LIM domains and LD motifs. In addition, Hic-5 localizes to focal adhesions and co-immunoprecipitates with PYK2/CAKbeta in vivo. Thus, Hic-5 may be a substrate for CAKbeta and play a role in signal transduction during proliferation, cell motility, and adhesion.
Molecular Weight 50 kDa

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Storage -20 °C
Supplier Images
anti-Transforming Growth Factor beta 1 Induced Transcript 1 (TGFB1I1) (AA 73-186) antibody Western blot analysis of Hic-5 on a rat lung lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti- Hic-5 antibody.
anti-Transforming Growth Factor beta 1 Induced Transcript 1 (TGFB1I1) (AA 73-186) antibody (2) Immunofluorescence staining of human fibroblasts.
anti-Transforming Growth Factor beta 1 Induced Transcript 1 (TGFB1I1) (AA 73-186) antibody (3) anti-Transforming Growth Factor beta 1 Induced Transcript 1 (TGFB1I1) (AA 73-186) antibody (Image 3)
Product cited in: Shibanuma, Mashimo, Kuroki et al.: "Characterization of the TGF beta 1-inducible hic-5 gene that encodes a putative novel zinc finger protein and its possible involvement in cellular senescence." in: The Journal of biological chemistry, Vol. 269, Issue 43, pp. 26767-74, 1994 (PubMed).

Matsuya, Sasaki, Aoto et al.: "Cell adhesion kinase beta forms a complex with a new member, Hic-5, of proteins localized at focal adhesions." in: The Journal of biological chemistry, Vol. 273, Issue 2, pp. 1003-14, 1998 (PubMed).

Jia, Ransom, Shibanuma et al.: "Identification and characterization of hic-5/ARA55 as an hsp27 binding protein." in: The Journal of biological chemistry, Vol. 276, Issue 43, pp. 39911-8, 2001 (PubMed).

Wang, Yang, Guo et al.: "Suppression of androgen receptor transactivation by Pyk2 via interaction and phosphorylation of the ARA55 coregulator." in: The Journal of biological chemistry, Vol. 277, Issue 18, pp. 15426-31, 2002 (PubMed).

Hosts (29), (5), (1)
Reactivities (34), (20), (20), (13), (12), (12)
Applications (19), (17), (10), (7), (6), (4), (3), (2), (2), (2), (2), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (4), (2), (1), (1), (1), (1), (1)
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