SATB Homeobox 1 (SATB1) (AA 550-667) antibody

Antigen: SATB Homeobox 1 (SATB1)

Synonyms: AW413156, 2610306G12Rik, SATB1, MGC165895

Binding Site: AA 550-667

Clonality: Monoclonal (14)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN968403

Additional Information

Alternative name: SATB1

Immunogen: Human SATB1

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Format: Liquid

Isotype: IgG1

Clone: 14

Description: Packaging of fully functional eukaryotic chromosomal DNA within the nucleus requires a highly ordered organizational process. Central to this is the formation of nucleosomes, which consist of octameric histone cores with defined segments of chromatin wound around them, and the interaction of nucleosomes with nuclear matrix components. Such specific genomic DNA segments that interact with the nuclear matrix are called scaffold or matrix attachment regions (SARs or MARs). SATB1 (Special AT-rich sequence-Binding protein 1), expressed predominantly in the thymus, is a homeodomain MAR binding protein. It recognizes ATC sequences that consist of stretches of A's, T's, and C's on one DNA strand. These sequences tend to unwind by extensive base-unpairing in response to negative superhelical strain. SATB1 exclusively binds double stranded DNA in the minor groove with minimal base contact and it has a low affinity for supercoiled DNA. In addition, SATB1 is thought to act as a transcriptional repressor. SATB1 is a MAR binding protein that binds to the base of chromatin loops and may be essential to T cell-specific gene regulation. This antibody is routinely tested by western blot analysis.
Synonyms: Special AT-rich sequence-Binding protein-1

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 106 kDa

Comments:

Related Products: ABIN968537, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Chromatin and Nuclear Signaling, Chromatin Binding Proteins, Transcription Factors

Restrictions: For Research Use only

Images

Western blot analysis of SATB1 on a Jurkat cell lysate (Human T-cell leukemia, ATCC TIB-152). Lane 1: 1:250, lane 2: 1: 500, lane 3: 1: 1000 dilution of the mouse anti- SATB1 antibody.

anti-SATB Homeobox 1 (SATB1) (AA 550-667) antibody (Image 2)

Publications

Product: Dickinson, Joh, Kohwi et al.: "A tissue-specific MAR/SAR DNA-binding protein with unusual binding site recognition." in: Cell, Vol. 70, Issue 4, pp. 631-45, 1992 (PubMed).

Liu, Bramblett, Zhu et al.: "The matrix attachment region-binding protein SATB1 participates in negative regulation of tissue-specific gene expression." in: Molecular and cellular biology, Vol. 17, Issue 9, pp. 5275-87, 1997 (PubMed).

de Belle, Cai, Kohwi-Shigematsu: "The genomic sequences bound to special AT-rich sequence-binding protein 1 (SATB1) in vivo in Jurkat T cells are tightly associated with the nuclear matrix at the bases of the chromatin loops." in: The Journal of cell biology, Vol. 141, Issue 2, pp. 335-48, 1998 (PubMed).