SATB Homeobox 1 (SATB1) (AA 550-667) antibody

Details for Product No. ABIN968403
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Antigen
Synonyms SATB1, 2610306G12Rik, AW413156
Epitope
AA 550-667
(16), (12), (10), (7), (5), (1), (1), (1), (1)
Reactivity
Human
(71), (39), (26), (14), (13), (13), (12)
Host
Mouse
(51), (16), (4)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(60), (27), (22), (18), (10), (10), (4), (4), (2), (2), (1), (1)
Pubmed 3 references available
Quantity 50 μg
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Catalog No. ABIN968403
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Immunogen Human SATB1
Clone Ghr2
Isotype IgG1
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name SATB1
Background Packaging of fully functional eukaryotic chromosomal DNA within the nucleus requires a highly ordered organizational process. Central to this is the formation of nucleosomes, which consist of octameric histone cores with defined segments of chromatin wound around them, and the interaction of nucleosomes with nuclear matrix components. Such specific genomic DNA segments that interact with the nuclear matrix are called scaffold or matrix attachment regions (SARs or MARs). SATB1 (Special AT-rich sequence-Binding protein 1), expressed predominantly in the thymus, is a homeodomain MAR binding protein. It recognizes ATC sequences that consist of stretches of A's, T's, and C's on one DNA strand. These sequences tend to unwind by extensive base-unpairing in response to negative superhelical strain. SATB1 exclusively binds double stranded DNA in the minor groove with minimal base contact and it has a low affinity for supercoiled DNA. In addition, SATB1 is thought to act as a transcriptional repressor. SATB1 is a MAR binding protein that binds to the base of chromatin loops and may be essential to T cell-specific gene regulation. This antibody is routinely tested by western blot analysis.
Synonyms: Special AT-rich sequence-Binding protein-1
Molecular Weight 106 kDa
Research Area Chromatin and Nuclear Signaling, Chromatin Binding Proteins, Transcription Factors
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-SATB Homeobox 1 (SATB1) (AA 550-667) antibody Western blot analysis of SATB1 on a Jurkat cell lysate (Human T-cell leukemia, ATCC TIB-152). Lane 1: 1:250, lane 2: 1: 500, lane 3: 1: 1000 dilution of the mouse anti- SATB1 antibody.
anti-SATB Homeobox 1 (SATB1) (AA 550-667) antibody (2) anti-SATB Homeobox 1 (SATB1) (AA 550-667) antibody (Image 2)
Product cited in: Dickinson, Joh, Kohwi et al.: "A tissue-specific MAR/SAR DNA-binding protein with unusual binding site recognition." in: Cell, Vol. 70, Issue 4, pp. 631-45, 1992 (PubMed).

Liu, Bramblett, Zhu et al.: "The matrix attachment region-binding protein SATB1 participates in negative regulation of tissue-specific gene expression." in: Molecular and cellular biology, Vol. 17, Issue 9, pp. 5275-87, 1997 (PubMed).

de Belle, Cai, Kohwi-Shigematsu: "The genomic sequences bound to special AT-rich sequence-binding protein 1 (SATB1) in vivo in Jurkat T cells are tightly associated with the nuclear matrix at the bases of the chromatin loops." in: The Journal of cell biology, Vol. 141, Issue 2, pp. 335-48, 1998 (PubMed).

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