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SATB1 antibody (SATB Homeobox 1) (AA 550-667)

Details for Product anti-SATB1 Antibody No. ABIN968403, Supplier: Log in to see
Antigen
  • SATB1
  • 2610306G12Rik
  • AW413156
Alternatives
anti-Human SATB1 antibody for Western Blotting
Epitope
AA 550-667
18
15
13
13
9
2
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
105
47
28
2
2
1
1
1
Host
Mouse
61
29
15
Clonality (Clone)
Monoclonal ()
Conjugate
This SATB1 antibody is un-conjugated
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
87
35
33
26
18
13
8
7
5
4
3
2
Supplier
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Immunogen Human SATB1 aa. 550-667
Clone 14-SATB1
Isotype IgG1
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name SATB1 (SATB1 Antibody Abstract)
Background Packaging of fully functional eukaryotic chromosomal DNA within the nucleus requires a highly ordered organizational process. Central to this is the formation of nucleosomes, which consist of octameric histone cores with defined segments of chromatin wound around them, and the interaction of nucleosomes with nuclear matrix components. Such specific genomic DNA segments that interact with the nuclear matrix are called scaffold or matrix attachment regions (SARs or MARs). SATB1 (Special AT-rich sequence-Binding protein 1), expressed predominantly in the thymus, is a homeodomain MAR binding protein. It recognizes ATC sequences that consist of stretches of A's, T's, and C's on one DNA strand. These sequences tend to unwind by extensive base-unpairing in response to negative superhelical strain. SATB1 exclusively binds double stranded DNA in the minor groove with minimal base contact and it has a low affinity for supercoiled DNA. In addition, SATB1 is thought to act as a transcriptional repressor. SATB1 is a MAR binding protein that binds to the base of chromatin loops and may be essential to T cell-specific gene regulation. This antibody is routinely tested by western blot analysis.
Synonyms: Special AT-rich sequence-Binding protein-1
Molecular Weight 106 kDa
Research Area Chromatin and Nuclear Signaling, Chromatin Binding Proteins, Transcription Factors
Pathways Caspase Cascade in Apoptosis
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-SATB1 antibody (SATB Homeobox 1) (AA 550-667) (ABIN968403) Western blot analysis of SATB1 on a Jurkat cell lysate (Human T-cell leukemia, ATCC T...
 image for anti-SATB1 antibody (SATB Homeobox 1) (AA 550-667) (ABIN968403) anti-SATB Homeobox 1 (SATB1) (AA 550-667) antibody (Image 2)
Product cited in: Beyer, Thabet, Müller et al.: "Repression of the genome organizer SATB1 in regulatory T cells is required for suppressive function and inhibition of effector differentiation. ..." in: Nature immunology, Vol. 12, Issue 9, pp. 898-907, 2011 (PubMed).

Alvarez, Yasui, Niida et al.: "The MAR-binding protein SATB1 orchestrates temporal and spatial expression of multiple genes during T-cell development." in: Genes & development, Vol. 14, Issue 5, pp. 521-35, 2000 (PubMed).

Cai, Kohwi-Shigematsu: "Intranuclear relocalization of matrix binding sites during T cell activation detected by amplified fluorescence in situ hybridization." in: Methods (San Diego, Calif.), Vol. 19, Issue 3, pp. 394-402, 2000 (PubMed).

de Belle, Cai, Kohwi-Shigematsu: "The genomic sequences bound to special AT-rich sequence-binding protein 1 (SATB1) in vivo in Jurkat T cells are tightly associated with the nuclear matrix at the bases of the chromatin loops." in: The Journal of cell biology, Vol. 141, Issue 2, pp. 335-48, 1998 (PubMed).

Liu, Bramblett, Zhu et al.: "The matrix attachment region-binding protein SATB1 participates in negative regulation of tissue-specific gene expression." in: Molecular and cellular biology, Vol. 17, Issue 9, pp. 5275-87, 1997 (PubMed).

Dickinson, Joh, Kohwi et al.: "A tissue-specific MAR/SAR DNA-binding protein with unusual binding site recognition." in: Cell, Vol. 70, Issue 4, pp. 631-45, 1992 (PubMed).