SATB1 antibody (Internal Region)

Catalog No. ABIN571004

Product Details anti-SATB1 Antibody

Target: SATB1 (SATB Homeobox 1 (SATB1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SATB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: SATB1

Sequence: ESDEENRQKT RPRT

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-ESDEENRQKTRPRT, from the internal region of the protein sequence according to NP_002962.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Thymus shows select nuclear staining. Recommended concentration: 3-5 μg/mL.

Western Blot: Approx. 90 kDa band observed in nuclear lysates of cell lines A431 and Jurkat and in lysates of cell lines U251 and Daudi (calculated MW of 89.0 kDa according to NP_001182399.1). Recommended concentration: 0.1-0.5 μg/mL. Primary incubation 1 h

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei of Jurkat cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SATB1

Target: SATB1 (SATB Homeobox 1 (SATB1))

Alternative Name: SATB1 (SATB1 Products)

Synonyms: 2610306G12Rik antibody, AW413156 antibody, SATB1 antibody, sb:cb955 antibody, si:ch211-195k18.2 antibody, SATB homeobox 1 antibody, special AT-rich sequence binding protein 1 antibody, SATB homeobox 1a antibody, SATB1 antibody, Satb1 antibody, satb1 antibody, satb1a antibody

Background: SATB1, SATB homeobox 1, DNA-binding protein SATB1, special AT-rich sequence binding protein 1, special AT-rich sequence binding protein 1 (binds to nuclear matrix/scaffold-associating DNA's)

Gene ID: 6304, 20230, 316164

NCBI Accession: NP_002962

Pathways: Caspase Cascade in Apoptosis, Activated T Cell Proliferation