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Citron (Rho-Interacting, serine/threonine Kinase 21) (CIT) (AA 1420-1612) antibody

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Synonyms CIT, crik, xcit, stk21, C030025P15Rik, CRIK, CRIK-SK, Cit-k, STK21
AA 1420-1612
(7), (7), (7), (1), (1), (1)
Human, Mouse (Murine), Rat (Rattus)
(26), (8), (7)
(24), (2)
Clonality (Clone)
Monoclonal ()
(2), (2), (2), (2), (2), (2)
Immunofluorescence (IF), Western Blotting (WB)
(20), (15), (10), (7), (1)
Pubmed 4 references available
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Quantity 50 μg
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Immunogen Mouse CRIK aa. 1420-1612
Clone 6-CRIK
Isotype IgG1, kappa
Cross-Reactivity Human, Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. For fluorochrome spectra and suitable instrument settings, please refer to us.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name CRIK (CIT Antibody Abstract)
Background Citron was identified by its interactions with activated Rho and Rac. It contains a zinc finger (ZF) region, a pleckstrin homology (PH) domain, a citron/MRCK/Gek (CMG) domain, and a coiled-coil domain with a Rho-Rac binding region (RRB). Citron binds to PSD-95 via its C-terminal tSXV motif and the PDZ domain on PSD-95. This association occurs at post synaptic densities where citron-PSD-95 may provide a specific link between the Rho signaling cascade and the synaptic NMDA receptor complex. Citron Rho-interacting kinase (CRIK) and CRIK short kinase (CRIK-SK) are Ser/Thr kinase isoforms of the myotonic dystrophy kinase family. CRIK contains full-length citron, with an N-terminal kinase domain. CRIK-SK is a splice variant that contains only the kinase domain. CRIK co-localizes with actin in keratinocytes and co-expression of CRIK and Rho increases CRIK kinase activity two-fold. In addition, CRIK mutants cause abnormal contraction during cytokinesis. CRIK is expressed highest in testis, but is also present in brain, spleen, lung, and kidney. Thus, CRIK is a Ser/Thr kinase that functions downstream of Rho during events such as cytokinesis and synaptic transmission.
Synonyms: Serine/Threonine Protein Kinase 21
Molecular Weight 240 kDa

Related Products: ABIN968539, ABIN967389, ABIN968537

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-Citron (Rho-Interacting, serine/threonine Kinase 21) (CIT) (AA 1420-1612) antibody (ABIN968502) Western blot analysis of CRIK on Rat PC12 cell lysate (ATCC CRL-1721). Lane 1: 1:500,...
Western Blotting (WB) image for anti-Citron (Rho-Interacting, serine/threonine Kinase 21) (CIT) (AA 1420-1612) antibody (ABIN968502) Western blot analysis of CRIK on Human Jurkat cell lysate (ATCC TIB-152). Lane 1: 1:2...
Product cited in: Furuyashiki, Fujisawa, Fujita et al.: "Citron, a Rho-target, interacts with PSD-95/SAP-90 at glutamatergic synapses in the thalamus." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 1, pp. 109-18, 1999 (PubMed).

Zhang, Vazquez, Apperson et al.: "Citron binds to PSD-95 at glutamatergic synapses on inhibitory neurons in the hippocampus." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 1, pp. 96-108, 1999 (PubMed).

Di Cunto, Calautti, Hsiao et al.: "Citron rho-interacting kinase, a novel tissue-specific ser/thr kinase encompassing the Rho-Rac-binding protein Citron." in: The Journal of biological chemistry, Vol. 273, Issue 45, pp. 29706-11, 1998 (PubMed).

Madaule, Eda, Watanabe et al.: "Role of citron kinase as a target of the small GTPase Rho in cytokinesis." in: Nature, Vol. 394, Issue 6692, pp. 491-4, 1998 (PubMed).

Catalog No. ABIN968502
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