Citron (Rho-Interacting, serine/threonine Kinase 21) (CIT) (AA 1420-1612) antibody

Details for Product No. ABIN968502
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms CIT, crik, xcit, stk21, C030025P15Rik, CRIK, CRIK-SK, Cit-k, STK21
Epitope
AA 1420-1612
(1)
Reactivity
Mouse (Murine)
(6), (3), (3)
Host
Mouse
(5), (1)
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB), Immunofluorescence (IF)
(5), (4), (2), (1)
Pubmed 4 references available
Quantity 50 µg
Options
Shipping to United States (Change)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN968502
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Mouse CRIK
Clone 6
Isotype IgG1
Cross-Reactivity Human, Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. For fluorochrome spectra and suitable instrument settings, please refer to us.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name CRIK
Background Citron was identified by its interactions with activated Rho and Rac. It contains a zinc finger (ZF) region, a pleckstrin homology (PH) domain, a citron/MRCK/Gek (CMG) domain, and a coiled-coil domain with a Rho-Rac binding region (RRB). Citron binds to PSD-95 via its C-terminal tSXV motif and the PDZ domain on PSD-95. This association occurs at post synaptic densities where citron-PSD-95 may provide a specific link between the Rho signaling cascade and the synaptic NMDA receptor complex. Citron Rho-interacting kinase (CRIK) and CRIK short kinase (CRIK-SK) are Ser/Thr kinase isoforms of the myotonic dystrophy kinase family. CRIK contains full-length citron, with an N-terminal kinase domain. CRIK-SK is a splice variant that contains only the kinase domain. CRIK co-localizes with actin in keratinocytes and co-expression of CRIK and Rho increases CRIK kinase activity two-fold. In addition, CRIK mutants cause abnormal contraction during cytokinesis. CRIK is expressed highest in testis, but is also present in brain, spleen, lung, and kidney. Thus, CRIK is a Ser/Thr kinase that functions downstream of Rho during events such as cytokinesis and synaptic transmission.
Synonyms: Serine/Threonine Protein Kinase 21
Molecular Weight 240 kDa
Comment

Related Products: ABIN968539, ABIN967389, ABIN968537

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Citron (Rho-Interacting, serine/threonine Kinase 21) (CIT) (AA 1420-1612) antibody Western blot analysis of CRIK on Rat PC12 cell lysate (ATCC CRL-1721). Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of anti-CRIK.
anti-Citron (Rho-Interacting, serine/threonine Kinase 21) (CIT) (AA 1420-1612) antibody (2) Western blot analysis of CRIK on Human Jurkat cell lysate (ATCC TIB-152). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-CRIK (5 min exposure).
Product cited in: Madaule, Eda, Watanabe et al.: "Role of citron kinase as a target of the small GTPase Rho in cytokinesis." in: Nature, Vol. 394, Issue 6692, pp. 491-4, 1998 (PubMed).

Di Cunto, Calautti, Hsiao et al.: "Citron rho-interacting kinase, a novel tissue-specific ser/thr kinase encompassing the Rho-Rac-binding protein Citron." in: The Journal of biological chemistry, Vol. 273, Issue 45, pp. 29706-11, 1998 (PubMed).

Zhang, Vazquez, Apperson et al.: "Citron binds to PSD-95 at glutamatergic synapses on inhibitory neurons in the hippocampus." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 1, pp. 96-108, 1999 (PubMed).

Furuyashiki, Fujisawa, Fujita et al.: "Citron, a Rho-target, interacts with PSD-95/SAP-90 at glutamatergic synapses in the thalamus." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 1, pp. 109-18, 1999 (PubMed).

Validation Images
Did you look for something else?
back to top