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Details for Product No. ABIN968572

Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (AA 70-181) antibody

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Antigen
Synonyms Darpp32, AU040756, DARPP-32, DARPP32, Darpp-32, darpp32, darpp-32
Epitope
»Alternatives AA 70-181
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunofluorescence (IF)
Pubmed 5 references available
Catalog no. ABIN968572
Quantity 50 µg
Price
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Immunogen Human DARPP-32
Clone Ghr5
Isotype IgG1
Cross-Reactivity Rat (Rattus), Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name DARPP-32
Background Dopaminergic signaling in midbrain neurons is essential to multiple brain functions and involves the activation of dopamine receptors,nsuch as D1 and D2, which regulate the phosphorylation state of DARPP-32 (dopamine and cyclic AMP-regulated phospho-protein of Mr = 32,000). D1 receptor ligation causes activation of PKA and phosphorylation of DARPP-32 at Thr-34, which converts DARPP-32 to a potent inhibitor of protein phosphatase 1 (PP1). In addition, DARPP-32 is converted to an inhibitor of PKA via phosphorylation at Thr-75 by cyclin-dependent kinase 5 (Cdk5). D2 receptor ligation inhibits PKA and activates protein phosphatase 2B/calcineurin causing dephosphorylation of DARPP-32. The major function of DARPP-32 may be to inhibit the activity of PP1, which controls the phosphorylation state of neurotransmitter receptors, ion channels, ion pumps, and transcription factors. DARPP-32 -/- mice are defective in the physiologicial and behavioral responses to dopamine. Thus, DARPP-32, a bifunctional signal transduction molecule that differentially controls a Ser/Thr kinase and a Ser/Thr phosphatase, is a critical element of dopaminergic neurotransmission and normal brain function.
Molecular Weight 32 kDa
Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Storage -20 °C
Product cited in: Kurihara, Lewis, Eisler et al.: "Cloning of cDNA for DARPP-32, a dopamine- and cyclic AMP-regulated neuronal phosphoprotein." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 8, Issue 2, pp. 508-17, 1988 (PubMed).

Fienberg, Hiroi, Mermelstein et al.: "DARPP-32: regulator of the efficacy of dopaminergic neurotransmission." in: Science (New York, N.Y.), Vol. 281, Issue 5378, pp. 838-42, 1998 (PubMed).

Yan, Hsieh-Wilson, Feng et al.: "Protein phosphatase 1 modulation of neostriatal AMPA channels: regulation by DARPP-32 and spinophilin." in: Nature neuroscience, Vol. 2, Issue 1, pp. 13-7, 1999 (PubMed).

Yan, Feng, Fienberg et al.: "D(2) dopamine receptors induce mitogen-activated protein kinase and cAMP response element-binding protein phosphorylation in neurons." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 20, pp. 11607-12, 1999 (PubMed).

Bibb, Snyder, Nishi et al.: "Phosphorylation of DARPP-32 by Cdk5 modulates dopamine signalling in neurons." in: Nature, Vol. 402, Issue 6762, pp. 669-71, 2000 (PubMed).

Alternatives for antigen "Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B)", type "Antibodies"
Hosts (126), (6), (2), (2)
Reactivities (115), (102), (89), (51), (49), (36), (36), (12), (4), (4), (2), (1), (1)
Applications (98), (45), (35), (30), (22), (21), (8), (6), (3), (2), (1), (1), (1)
Conjugates (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Epitopes (37), (28), (11), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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