Nestin (NES) (AA 402-604) antibody

Details for Product No. ABIN968628
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Antigen
Synonyms paranemin, NES, AA166324, C78523, ESTM46
Epitope
AA 402-604
(26), (15), (10), (8), (6), (6), (4), (4), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Rat (Rattus)
(146), (39), (34), (3), (3), (3), (2), (2), (2), (1)
Host
Mouse
(118), (56), (7), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(7), (5), (5), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application
Western Blotting (WB)
(126), (74), (47), (44), (42), (37), (32), (20), (20), (12), (10), (6), (2), (1)
Pubmed 5 references available
Quantity 50 µg
Options
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Catalog No. ABIN968628
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Immunogen Rat Nestin Recombinant Protein
Clone 25
Isotype IgG1, kappa
Cross-Reactivity Human
Characteristics 1. Please refer to us for technical protocols.
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Nestin
Background The cytoskeleton consists primarily of core structural proteins that include microfilaments, microtubules, and intermediate filaments (IFs). IFs contain more than 50 distinct proteins that are organized into six different subtypes: Type I/II keratins expressed in epithelia, type III vimentin/desmin, type IV neurofilament proteins, type V nuclear lamins, and type VI nestin expressed primarily in embryonic cells. Nestin has a conserved core region (amino acids 7 to 314), which contains an alpha helical domain that is involved in coiled-coil assembly of IFs. The C-terminal region of nestin is similar to type IV IFs, since it contains highly charged amino acids, many glutamate residues, and an 11 amino acid repeat motif. Nestin is expressed in the cerebrum during embryonic development, in the cerebellum during early postnatal development, and in dermatomal cells and myoblasts during myogenesis. In vitro, nestin forms homodimers and homotetramers, but not IFs, and can co-assemble with type III vimentin and type IV internexin proteins. Thus, nestin is a core IF protein that is essential for proper cytoskeletal formation during neurogenesis and myogenesis.
Molecular Weight 200-260 kDa
Research Area Angiogenesis, Lineage Markers
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Nestin (NES) (AA 402-604) antibody Western blot analysis of Nestin on a rat (E17) cerebrum lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-Nestin antibody.
Product cited in: Lendahl, Zimmerman, McKay: "CNS stem cells express a new class of intermediate filament protein." in: Cell, Vol. 60, Issue 4, pp. 585-95, 1990 (PubMed).

Kachinsky, Dominov, Miller: "Myogenesis and the intermediate filament protein, nestin." in: Developmental biology, Vol. 165, Issue 1, pp. 216-28, 1994 (PubMed).

Steinert, Chou, Prahlad et al.: "A high molecular weight intermediate filament-associated protein in BHK-21 cells is nestin, a type VI intermediate filament protein. Limited co-assembly in vitro to form heteropolymers with type III vimentin and type IV alpha-internexin." in: The Journal of biological chemistry, Vol. 274, Issue 14, pp. 9881-90, 1999 (PubMed).

Kernie, Erwin, Parada: "Brain remodeling due to neuronal and astrocytic proliferation after controlled cortical injury in mice." in: Journal of neuroscience research, Vol. 66, Issue 3, pp. 317-26, 2001 (PubMed).

Jin, Zhu, Sun et al.: "Vascular endothelial growth factor (VEGF) stimulates neurogenesis in vitro and in vivo." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 18, pp. 11946-50, 2002 (PubMed).

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