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Nestin antibody (NES) (AA 402-604)

Details for Product anti-NES Antibody No. ABIN968628, Supplier: Log in to see
Antigen
  • paranemin
  • NES
  • AA166324
  • C78523
  • ESTM46
Epitope
AA 402-604
26
15
15
14
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12
9
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3
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2
2
1
1
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1
1
1
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1
Reactivity
Human, Rat (Rattus)
191
57
57
4
4
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3
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2
1
1
Host
Mouse
151
78
7
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1
Clonality (Clone)
Monoclonal ()
Conjugate
This Nestin antibody is un-conjugated
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3
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2
2
1
1
1
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1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
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93
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54
47
42
27
13
12
11
4
4
3
3
2
1
1
1
1
Supplier
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Immunogen Rat Nestin aa. 402-604 Recombinant Protein
Clone 25-NESTIN
Isotype IgG1, kappa
Cross-Reactivity Human
Characteristics 1. Please refer to us for technical protocols.
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Nestin (NES Antibody Abstract)
Background The cytoskeleton consists primarily of core structural proteins that include microfilaments, microtubules, and intermediate filaments (IFs). IFs contain more than 50 distinct proteins that are organized into six different subtypes: Type I/II keratins expressed in epithelia, type III vimentin/desmin, type IV neurofilament proteins, type V nuclear lamins, and type VI nestin expressed primarily in embryonic cells. Nestin has a conserved core region (amino acids 7 to 314), which contains an alpha helical domain that is involved in coiled-coil assembly of IFs. The C-terminal region of nestin is similar to type IV IFs, since it contains highly charged amino acids, many glutamate residues, and an 11 amino acid repeat motif. Nestin is expressed in the cerebrum during embryonic development, in the cerebellum during early postnatal development, and in dermatomal cells and myoblasts during myogenesis. In vitro, nestin forms homodimers and homotetramers, but not IFs, and can co-assemble with type III vimentin and type IV internexin proteins. Thus, nestin is a core IF protein that is essential for proper cytoskeletal formation during neurogenesis and myogenesis.
Molecular Weight 200-260 kDa
Research Area Angiogenesis, Lineage Markers
Pathways
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-Nestin antibody (NES) (AA 402-604) (ABIN968628) Western blot analysis of Nestin on a rat (E17) cerebrum lysate. Lane 1: 1:500, lane 2...
Product cited in: Jin, Zhu, Sun et al.: "Vascular endothelial growth factor (VEGF) stimulates neurogenesis in vitro and in vivo." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 18, pp. 11946-50, 2002 (PubMed).

Kernie, Erwin, Parada: "Brain remodeling due to neuronal and astrocytic proliferation after controlled cortical injury in mice." in: Journal of neuroscience research, Vol. 66, Issue 3, pp. 317-26, 2001 (PubMed).

Steinert, Chou, Prahlad et al.: "A high molecular weight intermediate filament-associated protein in BHK-21 cells is nestin, a type VI intermediate filament protein. Limited co-assembly in vitro to form heteropolymers with type III vimentin and type IV alpha-internexin." in: The Journal of biological chemistry, Vol. 274, Issue 14, pp. 9881-90, 1999 (PubMed).

Kachinsky, Dominov, Miller: "Myogenesis and the intermediate filament protein, nestin." in: Developmental biology, Vol. 165, Issue 1, pp. 216-28, 1994 (PubMed).

Lendahl, Zimmerman, McKay: "CNS stem cells express a new class of intermediate filament protein." in: Cell, Vol. 60, Issue 4, pp. 585-95, 1990 (PubMed).