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Nestin antibody (FITC)

NES Reactivity: Human ICC, IF Host: Mouse Monoclonal 10C2 FITC
Independent Validation (1 validation)
Catalog No. ABIN1774766
$851.51
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50 μL
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  • Target See all Nestin (NES) Antibodies
    Nestin (NES)
    Reactivity
    • 136
    • 71
    • 47
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 108
    • 36
    • 4
    • 2
    • 1
    Mouse
    Clonality
    • 114
    • 37
    Monoclonal
    Conjugate
    • 66
    • 11
    • 9
    • 8
    • 7
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    This Nestin antibody is conjugated to FITC
    Application
    • 71
    • 58
    • 50
    • 48
    • 40
    • 39
    • 38
    • 28
    • 27
    • 16
    • 8
    • 6
    • 2
    • 2
    • 1
    • 1
    Immunocytochemistry (ICC), Immunofluorescence (IF)
    Specificity
    This antibody recognises nestin, a member of the intermediate filament family required for brain and eye development.
    Purification
    Protein G purified
    Immunogen
    A 150-amino-acid fragment from the cloned human nestin conjugated to GST
    Clone
    10C2
    Isotype
    IgG1
  • Application Notes
    Optimal antibody dilution should be determined by titration.
    Restrictions
    For Research Use only
  • Validation #029824 (Immunofluorescence)
    'Independent Validation' Badge
    by
    Ensigna Biosystems
    No.
    #029824
    Date
    11/06/2014
    Antigen
    Lot Number
    I030314
    Method validated
    Immunofluorescence
    Positive Control
    PC-3 cells
    Negative Control
    MCF-7 cells
    Notes
    Faint fluorescent signal was detected in the positive control cells. No signal was detected in the negative control cells or isotype negative control.
    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    • Antigen: Nestin (NES) (FITC)
    • Catalog number: ABIN1774766
    • Lot number: I030314
    • Dilution: 1:75, 1:100
    Secondary Antibody
    Full Protocol
    • Prostate and breast adenocarcinoma cell lines were grown directly on chamber slides, washed with 1X PBS, and fixed with 4% paraformaldehyde in 1X PBS for 15 min at room temperature (RT).
    • Fixed cells were rinsed three times in PBS for 5 min each at RT.
    • Cells were blocked in 1X PBS / 5% normal goat serum / 0.3% Triton X-100 for 60 min at RT.
    • Cells were rinsed two times in 1X PBS.
    • Cells were incubated with primary antibody diluted 1:75 and 1:100 in 1X PBS / 1% BSA / 0.3% Triton X-100 overnight at 4°C in dark conditions.
    • Cells were rinsed three times in PBS for 5 min each at RT.
    • Coverslips were mounted on slides with DAPI-Fluoromount-G.
    • Stained cells were imaged with a Nikon Eclipse E600 microscope and Olympus DP70 camera.
    Experimental Notes
    The anti-Nestin antibody showed positive staining of weak intensity localized appropriately to the cell cytoplasm in a pattern consistent with intermediate filaments in positive control PC3 cells. No staining was seen in the negative control MCF7 cells or with the isotype control antibody. Although specificity of the antibody was acceptable, its sensitivity with the current protocol was questionable. It is, however, entirely possible that if this antibody is used with an appropriate amplification protocol the sensitivity would be increased.
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Purified IgG conjugated to Fluoroscein using Innova Biosciences Lightning-Link®, supplied in Phosphate buffered saline (PBS) containing 0.09 % Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Target
    Nestin (NES)
    Alternative Name
    Nestin (NES Products)
    Synonyms
    paranemin antibody, NES antibody, AA166324 antibody, C78523 antibody, ESTM46 antibody, nestin antibody, nestin S homeolog antibody, NES antibody, nes.S antibody, Nes antibody, nes antibody
    Background
    Neural Stem Cell Marker
    Gene ID
    407242
    OMIM
    600915
    UniProt
    P18175
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