TRAF2 and NCK Interacting Kinase (TNIK) (AA 522-644) antibody

Antigen: TRAF2 and NCK Interacting Kinase (TNIK)

Synonyms: TNIK, AI451411, KIAA0551, MGC189819, MGC189859, 1500031A17Rik, 4831440I19Rik, C530008O15Rik, C630040K21Rik

Binding Site: AA 522-644

Clonality: Monoclonal (53)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN968788

Additional Information

Alternative name: TNIK

Immunogen: Human TNIK

Cross-Reactivity: Rat (Rattus), Mouse (Murine)

Format: Liquid

Isotype: IgG1

Clone: 53

Description: Ste20 is a S. cerevisiae Ser/Thr protein kinase that functions upstream of the MAP kinase module. Mammalian and yeast homologs of this kinase are divided into two classes based on their structure and regulation. Members of the first class (Ste20, Cla4, and p21-activated protein kinase) contain a C-terminal kinase domain, an N-terminal regulatory domain and a small GTPase Rac1/Cdc42 binding domain. Members of the second class lack the GTPase binding sites, but are similar to the former class throughout the catalytic domain. This second class includes germinal center kinase (GCK), HPK, KHS, KRS1 and 2, MST1, 2, and 3, NIK, SOK-1, and TNIK. Traf2- and NCK-interacting kinase (TNIK) is most homologous to another NCK-interacting kinase, NIK. TNIK contains an N- terminal kinase domain, and a C-terminal GCK homology (GCKH) domain. The mRNA of TNIK is expressed highest in heart, brain, and skeletal muscle. Overexpression of TNIK activates the JNK pathway, and leads to the disruption of F-actin structures and the inhibition of cell spreading. In vitro, TNIK can phosphorylate gelsolin. Thus, TNIK is a GCK family kinase that may regulate both the JNK pathway and cytoskeletal dynamics.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 150 kDa

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Signaling, Extracellular Matrix, Kinases/Phosphatases

Restrictions: For Research Use only

Images

Western blot analysis of TNIK of 293 HEK lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of TNIK.

Publications

Product: Fu, Shen, Huang et al.: "TNIK, a novel member of the germinal center kinase family that activates the c-Jun N-terminal kinase pathway and regulates the cytoskeleton." in: The Journal of biological chemistry, Vol. 274, Issue 43, pp. 30729-37, 1999 (PubMed).