Bone Morphogenetic Protein Receptor, Type II (serine/threonine Kinase) (BMPR2) (AA 803-996) antibody
|Synonyms||BMR2, PPH1, BMPR3, BRK-3, T-ALK, BMPR-II, FLJ41585, FLJ76945, BMP-2, BMPRII, AL117858, AW546137, BB189135, 2610024H22Rik, Bmpr-II, BMPR-2, BMPR2|
Alternatives Western Blotting (WB)
|3 references available|
|Quantity||50 µg (250 µg/ml)|
|Price||Product not available in this region.|
|Cross-Reactivity||Human, Rat (Rattus)|
|Description||The transforming growth factor beta (TGFbeta)/activin/BMP family of growth factor plays a diverse and important role in growth, development, and differentiation. The receptors for this famiy of proteins are type I and II ser/thr kinase receptors. Bone morphogenetic protein receptors (BMPRs) include two type I receptors, BMPR-IA and BMPR-IB, and a type II receptor, BMPR-II. BMPR-II is a widely expressed receptor, with high mRNA expression during development in many tissues. BMPR-II transfected COS-1 cells show binding to BMP-7 (osteogenic protein-1) and BMP-4, and co-transfection with the type I receptor, ActR-I, leads to transcriptional activation in response to BMP-7. In addition, extracellular matrix proteins may regulate the expression of BMPR-II during angiogenesis, since fibrillar type I collagen induces BMPR-II expression in endothelial cells. In familial primary pulmonary hypertension, the BMPR-II gene is mutated in a manner that may disrupt ligand binding, kinase activity, and heteromeric dimer formation. Thus, BMPR-II may be an important mediator of BMP effects during growth and differentiation of endothelial cells.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||130 kDa|
Related Products: ABIN968540, ABIN967389
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20°C.|
|Restrictions||For Research Use only|
|Western blot analysis of BMPR-II on mouse cerebrum lysate. Lane 1: 1:250, lane 2 :1:500, lane 3: 1:1000 dilution of anti-BMPR-II antibody|
Rosenzweig, Imamura, Okadome et al.: "Cloning and characterization of a human type II receptor for bone morphogenetic proteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 17, pp. 7632-6, 1995 (PubMed).
, Lane, Machado et al.: "Heterozygous germline mutations in BMPR2, encoding a TGF-beta receptor, cause familial primary pulmonary hypertension." in: Nature genetics, Vol. 26, Issue 1, pp. 81-4, 2000 (PubMed).
Regazzoni, Winterhalter, Rohrer: "Type I collagen induces expression of bone morphogenetic protein receptor type II." in: Biochemical and biophysical research communications, Vol. 283, Issue 2, pp. 316-22, 2001 (PubMed).
|Hosts||Rabbit (31), Mouse (9)|
|Reactivities||Human (39), Rat (Rattus) (34), Mouse (Murine) (33), Cow (Bovine) (20), Chicken (19), Pig (Porcine) (19), Horse (Equine) (18), Monkey (8), Cat (Feline) (1), Dog (Canine) (1), Rabbit (1)|
|Applications||Western Blotting (WB) (24), ELISA (23), Immunofluorescence (IF) (23), Immunohistochemistry (IHC) (10), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoprecipitation (IP) (3), Flow Cytometry (FACS) (2), Immunocytochemistry (ICC) (2), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||N-Term (3), AA 34-49 (1)|