Bone Morphogenetic Protein Receptor, Type II (serine/threonine Kinase) (BMPR2) (AA 803-996) antibody

Antigen: Bone Morphogenetic Protein Receptor, Type II (serine/threonine Kinase) (BMPR2)

Synonyms: BMR2, PPH1, BMPR3, BRK-3, T-ALK, BMPR-II, FLJ41585, FLJ76945, BMP-2, BMPRII, AL117858, AW546137, BB189135, 2610024H22Rik, Bmpr-II, BMPR-2, BMPR2

Binding Site: AA 803-996

Clonality: Monoclonal (18)

Host: Mouse

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN968806

Additional Information

Alternative name: BMPR-II

Immunogen: Mouse BMPR-II

Cross-Reactivity: Human, Rat (Rattus)

Format: Liquid

Isotype: IgG1

Clone: 18

Description: The transforming growth factor beta (TGFbeta)/activin/BMP family of growth factor plays a diverse and important role in growth, development, and differentiation. The receptors for this famiy of proteins are type I and II ser/thr kinase receptors. Bone morphogenetic protein receptors (BMPRs) include two type I receptors, BMPR-IA and BMPR-IB, and a type II receptor, BMPR-II. BMPR-II is a widely expressed receptor, with high mRNA expression during development in many tissues. BMPR-II transfected COS-1 cells show binding to BMP-7 (osteogenic protein-1) and BMP-4, and co-transfection with the type I receptor, ActR-I, leads to transcriptional activation in response to BMP-7. In addition, extracellular matrix proteins may regulate the expression of BMPR-II during angiogenesis, since fibrillar type I collagen induces BMPR-II expression in endothelial cells. In familial primary pulmonary hypertension, the BMPR-II gene is mutated in a manner that may disrupt ligand binding, kinase activity, and heteromeric dimer formation. Thus, BMPR-II may be an important mediator of BMP effects during growth and differentiation of endothelial cells.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 130 kDa

Comments:

Related Products: ABIN968540, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20°C.

Restrictions: For Research Use only

Images

Western blot analysis of BMPR-II on mouse cerebrum lysate. Lane 1: 1:250, lane 2 :1:500, lane 3: 1:1000 dilution of anti-BMPR-II antibody

Publications

Product: Rosenzweig, Imamura, Okadome et al.: "Cloning and characterization of a human type II receptor for bone morphogenetic proteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 17, pp. 7632-6, 1995 (PubMed).

, Lane, Machado et al.: "Heterozygous germline mutations in BMPR2, encoding a TGF-beta receptor, cause familial primary pulmonary hypertension." in: Nature genetics, Vol. 26, Issue 1, pp. 81-4, 2000 (PubMed).

Regazzoni, Winterhalter, Rohrer: "Type I collagen induces expression of bone morphogenetic protein receptor type II." in: Biochemical and biophysical research communications, Vol. 283, Issue 2, pp. 316-22, 2001 (PubMed).