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Details for Product No. ABIN968809

N-Acylsphingosine Amidohydrolase (Acid Ceramidase) 1 (ASAH1) (AA 88-182) antibody

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Antigen
Synonyms 2310081N20Rik, AC, Asah, ACDase, ASAH, PHP, PHP32, SMAPME, MGC82286, asah1, zgc:101637, ASAH1
Epitope
»Alternatives AA 88-182
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB)
Pubmed 3 references available
Catalog no. ABIN968809
Quantity 50 µg
Price
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Immunogen Human Acid Ceramidase
Clone 23
Isotype IgG1
Cross-Reactivity Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name Acid Ceramidase
Background Ceramide is a sphingolipid that exhibits a wide variety of functions, including monocyte differentiation, apoptosis, neurite outgrowth, and Ca2+ transport. It also serves as the precursor of many sphingolipids and anchors these into the outer leaflet of the plasma membrane via hydrophobic interactions. Acid ceramidase is a lysosomal enzyme that was purified from human urine. It is synthesized as a 55kDa precursor protein, which is then processesed into the mature alpha-subunit (13kDa) and beta-subunit (40kDa). Acid ceramidase catalyzes the hydrolysis of ceramide into free fatty acid and sphingosine. Sphingosine, and its phosphorylated form, sphingosine-1-phosphate (SPP), have been shown to inhibit PKC activity and act as a second messenger in cell proliferation and differentiation. Acid ceramidase is also the cause of a lysosomal storage disorder known as Farber’s disease. This disease is characterized by an accumulation of ceramide in tissues, leading to swelling and pain of the joints and extremities, pulmonary insufficiency, and death at an early age. Thus, acid ceramidase is necessary in the hydrolysis of ceramide and is the cause of Farber’s disease.
Molecular Weight 13 kDa
Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Storage -20 °C
Product cited in: Bernardo, Hurwitz, Zenk et al.: "Purification, characterization, and biosynthesis of human acid ceramidase." in: The Journal of biological chemistry, Vol. 270, Issue 19, pp. 11098-102, 1995 (PubMed).

Strelow, Bernardo, Adam-Klages et al.: "Overexpression of acid ceramidase protects from tumor necrosis factor-induced cell death." in: The Journal of experimental medicine, Vol. 192, Issue 5, pp. 601-12, 2000 (PubMed).

Ferlinz, Kopal, Bernardo et al.: "Human acid ceramidase: processing, glycosylation, and lysosomal targeting." in: The Journal of biological chemistry, Vol. 276, Issue 38, pp. 35352-60, 2001 (PubMed).

Alternatives for antigen "N-Acylsphingosine Amidohydrolase (Acid Ceramidase) 1 (ASAH1)", type "Antibodies"
Hosts (35), (7)
Reactivities (37), (22), (22), (13), (13), (12), (12), (2)
Applications (33), (20), (13), (10), (8), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (7), (4), (2), (2), (1), (1), (1)
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