Damage-Specific DNA Binding Protein 1, 127kDa (DDB1) (AA 739-935) antibody

Details for Product No. ABIN968852
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Antigen
Synonyms DDB1, xpe, ddba, xap1, xpce, xpe-bf, uv-ddb1, ddb1, 127kDa, AA408517, p127-Ddb1, DDBA, UV-DDB1, XAP1, XPCE, XPE, XPE-BF
Epitope
AA 739-935
(10), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Human
(55), (25), (21), (15), (12), (3), (2), (1), (1)
Host
Mouse
(38), (9), (8)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF)
(45), (16), (16), (12), (10), (10), (8), (5), (4), (1), (1), (1), (1)
Pubmed 4 references available
Quantity 50 μg
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Catalog No. ABIN968852
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Immunogen Human DDB1
Clone 8
Isotype IgG1
Cross-Reactivity Rat (Rattus), Mouse (Murine), Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name DDB1
Background DNA lesions caused by chemical mutagens or radiation are corrected via nucleotide excision repair (NER). The NER system includes multiple proteins involved in xeroderma pigmentosum (XP) disorders, a pathology that causes hypersensitivity to sunlight and higher incidence of skin cancer. The proteins that cause these disorders are XP proteins and include XPA, XPB, XPC, XPD, XPF, and XPG. There are six repair complexes in the NER system composed of 15-18 proteins that include XPA, XPC, XPF, TFIIH, and hHR23B. In addition to these proteins, UV damage DNA-binding (UV-DDB) protein activity has also been associated with the NER system due to the fact that UV-DDB activity is absent in a subset of XPE Ddb- patients. UV-DDB consists of two subunits, DDB1 and DDB2, which can be injected into XPE cells to restore DNA repair synthesis. UV-DDB activity may be involved in the early stages of NER when it may promote recognition of the damaged DNA through DDB2. In addition, DDB1 can bind the histone acetyltransferase p300, which may be important for chromatin remodeling during the early stages of NER. Thus, UV-DDB activity may be important for recognition of specific types of DNA damage during NER.
Molecular Weight 127 kDa
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Comment

Related Products: ABIN968535, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Damage-Specific DNA Binding Protein 1, 127kDa (DDB1) (AA 739-935) antibody Immunofluorescence staining of A431 cells (Human epithelial carcinoma, ATCC CRL-1555).
anti-Damage-Specific DNA Binding Protein 1, 127kDa (DDB1) (AA 739-935) antibody (2) Western blot analysis of DDB1 on a HeLa cell lysate (Human cervical epitheloid carcinoma, ATCC CCL-2.2). Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-DDB1 antibody.
Product cited in: Dualan, Brody, Keeney et al.: "Chromosomal localization and cDNA cloning of the genes (DDB1 and DDB2) for the p127 and p48 subunits of a human damage-specific DNA binding protein." in: Genomics, Vol. 29, Issue 1, pp. 62-9, 1996 (PubMed).

Fujiwara, Masutani, Mizukoshi et al.: "Characterization of DNA recognition by the human UV-damaged DNA-binding protein." in: The Journal of biological chemistry, Vol. 274, Issue 28, pp. 20027-33, 1999 (PubMed).

Rapić-Otrin, McLenigan, Bisi et al.: "Sequential binding of UV DNA damage binding factor and degradation of the p48 subunit as early events after UV irradiation." in: Nucleic acids research, Vol. 30, Issue 11, pp. 2588-98, 2002 (PubMed).

Sun, Lu, Chao: "Identification of rat DDB1, a putative DNA repair protein, and functional correlation with its damaged-DNA recognition activity." in: Journal of biomedical science, Vol. 9, Issue 4, pp. 371-80, 2002 (PubMed).

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