Gephyrin (GPHN) (AA 569-726) antibody

Details for Product No. ABIN968898
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Antigen
Synonyms GPHN, gpgb1, DKFZp459M0825, GEPH, GPH, GPHRYN, HKPX1, 5730552E08Rik, AI662856, BC027112, C230040D23, geph, Geph
Epitope
AA 569-726
(8), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Rat (Rattus)
(53), (23), (20), (14), (13), (12), (3), (2), (2), (1)
Host
Mouse
(36), (19), (2), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)
(48), (26), (13), (10), (9), (7), (7), (4), (1), (1)
Pubmed 2 references available
Quantity 50 µg
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Catalog No. ABIN968898
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Immunogen Rat Gephyrin
Clone DF7
Isotype IgG1
Cross-Reactivity Human, Mouse (Murine), Chicken, Fruit Fly (Drosophila melanogaster)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Gephyrin
Background The sub-membraneous region of the postsynaptic neuron is an intricate network of cytoskeletal elements generally known as the postsynaptic density. It is thought that this elaborate cytoskeletal region is critical for receptor targeting, clustering, and efficient signal input. Gephyrin, a 93 kDa protein, was identified as a result of its ability to bind to polymerized tubulin (microtubules). Although expressed in all tissues, gephyrin mRNA is found most abundantly in brain. Alternative splicing produces at least five different mRNAs. Gephyrin colocalizes and interacts with the glycine receptor at the postsynaptic density. It is possible that Gephyrin acts as an anchor between the glycine receptor and microtubules.
Molecular Weight 93 kDa
Research Area Neurology
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Gephyrin (GPHN) (AA 569-726) antibody Western blot analysis of Gephyrin. Lysates from rat cerebrum (5 µg/lane), S2 cells (7.5 µg/lane), and 0-12 hours Drosophila embryos (7.5 µg/lane) were probed with anti- Gephyrin at concentrations of 4 µg/ml (lane 1), 2 µg/ml (lane 2), and 1 µg/ml (lane 3). Anti-Gephyrin detects a band of 93 kDa in rat cerebrum and 74 kDa in S2 cells and Drosophila embryos.
anti-Gephyrin (GPHN) (AA 569-726) antibody (2) Gephyrin immunochemical staining of 0-12 hour embryos. Formalin-fixed embryos kept in ethanol at -20°C, were re-hydrated and incubated with anti-Gephyrin at 1 µgg/ml in TBST. After extensive washes to remove unbound antibody, Gephyrin signal was visualized with ALEXA fluor 488 secondary antibody (1/300) and confocal microscopy. Gephyrin staining is found throughout the cellularizing embryo, and it appears more intense in the nuclei with nuclear dots stained.
anti-Gephyrin (GPHN) (AA 569-726) antibody (3) High magnification view of A.
Product cited in: Prior, Schmitt, Grenningloh et al.: "Primary structure and alternative splice variants of gephyrin, a putative glycine receptor-tubulin linker protein." in: Neuron, Vol. 8, Issue 6, pp. 1161-70, 1992 (PubMed).

Meyer, Kirsch, Betz et al.: "Identification of a gephyrin binding motif on the glycine receptor beta subunit." in: Neuron, Vol. 15, Issue 3, pp. 563-72, 1995 (PubMed).

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