GA Binding Protein Transcription Factor, alpha Subunit 60kDa (GABPA) (AA 120-190) antibody

Antigen: GA Binding Protein Transcription Factor, alpha Subunit 60kDa (GABPA)

Synonyms: NFT2, NRF2, NRF2A, E4TF1A, E4TF1-60, Nrf2a, Nrf-2alpha, gabpa-A, XrpFI, X-alpha1, GABPA, gabpa, MGC84637, MGC142634, nft2, nrf2, nrf2a, e4tf1a, e4tf1-60

Binding Site: AA 120-190

Clonality: Monoclonal (8C1B10)

Host: Mouse

Reactivity: Human, Mouse (Murine)

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Catalog no.: ABIN969163

Additional Information

Alternative name: GABPA

Gene ID: 2551

Immunogen: Purified recombinant fragment of human GABPA (AA 120-190) expressed in E. Coli.

Format: Liquid

Isotype: IgG1

Clone: 8C1B10

Description: GABPA: GA binding protein transcription factor, alpha subunit 60kDa. It is one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, it may play a role in the Down Syndrome phenotype.
Synonyms: NFT2, NRF2

Molecular Weight: 51kDa

Application Details

Application Notes: Western Blotting: 1/500 - 1/2000.
Immunofluorescence: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Purification: Ascites

Buffer: Ascitic fluid containing 0.03% sodium azide.

Preservative: 0.03% sodium azide

Storage: Store at 4℃, for long term storage, store at -20℃.

Research Area: Chromatin and Nuclear Signaling, Transcription Factors

Restrictions: For Research Use only

Publications

Product: Batchelor, Piper, de la Brousse et al.: "The structure of GABPalpha/beta: an ETS domain- ankyrin repeat heterodimer bound to DNA." in: Science (New York, N.Y.), Vol. 279, Issue 5353, pp. 1037-41, 1998 (PubMed).

Sawada, Simizu, Suzuki et al.: "Synergistic transcriptional activation by hGABP and select members of the activation transcription factor/cAMP response element-binding protein family." in: The Journal of biological chemistry, Vol. 274, Issue 50, pp. 35475-82, 2000 (PubMed).

Vogel, Kristie: "The novel coactivator C1 (HCF) coordinates multiprotein enhancer formation and mediates transcription activation by GABP." in: The EMBO journal, Vol. 19, Issue 4, pp. 683-90, 2000 (PubMed).