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Death-Domain Associated Protein (DAXX) antibody

Details for Product No. ABIN969493, Supplier: Login to see
Antigen
  • im:6905684
  • LOC100226911
  • BING2
  • DAP6
  • EAP1
Reactivity
Human
259
73
60
10
3
1
1
Host
Mouse
215
49
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
9
9
9
6
6
6
6
6
6
6
6
6
3
3
3
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
189
79
66
46
44
37
25
20
10
8
8
6
5
2
1
1
Supplier
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Immunogen Purified recombinant fragment of human DAXX expressed in E. coli.
Clone 7A11
Isotype IgG1
Purification Purified by protein G affinity chromatography
Alternative Name DAXX (DAXX Antibody Abstract)
Background DAXX (death-domain associated protein), it is a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants.
Molecular Weight 81 kDa
Gene ID 1616
HGNC 1616
Pathways
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 50 % glycerol and 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
Flow Cytometry (FACS) image for anti-Death-Domain Associated Protein (DAXX) antibody (ABIN969493) Figure 1: Flow cytometric analysis of Hela cells using DAXX mouse mAb (green) and neg...
Immunocytochemistry (ICC) image for anti-Death-Domain Associated Protein (DAXX) antibody (ABIN969493) Figure 2: Confocal immunofluorescence analysis of PANC-1 cells using DAXX mouse mAb (...
Immunocytochemistry (ICC) image for anti-Death-Domain Associated Protein (DAXX) antibody (ABIN969493) Figure 3: Confocal immunofluorescence analysis of Hela cells using DAXX mouse mAb (gr...
Western Blotting (WB) image for anti-Death-Domain Associated Protein (DAXX) antibody (ABIN969493) Figure 4: Western blot analysis using DAXX mouse mAb against K562 cell lysate (1) .
Background publications Beausoleil, Jedrychowski, Schwartz et al.: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004 (PubMed).

Liu, Kojima, Kurosawa et al.: "Regulated expression system for GD3 synthase cDNA and induction of differentiation in Neuro2a cells." in: Glycobiology, Vol. 7, Issue 8, pp. 1067-76, 1998 (PubMed).