Death-Domain Associated Protein (DAXX) antibody

Details for Product No. ABIN969493
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Antigen
Synonyms im:6905684, LOC100226911, BING2, DAP6, EAP1
Reactivity
Human
(194), (65), (52), (48), (37), (24), (5), (4)
Host
Mouse
(170), (31)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(8), (8), (8), (6), (6), (6), (6), (6), (6), (6), (6), (2), (2), (2)
Application
ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Fluorescence Microscopy (FM)
(135), (65), (60), (36), (35), (34), (22), (10), (8), (7), (5), (2), (1)
Pubmed 1 reference available
Quantity 100 µL
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Catalog No. ABIN969493
363.00 $
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Immunogen Purified recombinant fragment of human DAXX expressed in E. coli.
Clone 7A11
Isotype IgG1
Purification Purified by protein G affinity chromatography
Alternative Name DAXX
Background Synonyms:
DAP6, EAP1, BING2
DAXX (death-domain associated protein), it is a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants.
Molecular Weight 81 kDa
Gene ID 1616
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 50 % glycerol and 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
anti-Death-Domain Associated Protein (DAXX) antibody Figure 4: Flow cytometric analysis of Hela cells using DAXX mouse mAb (green) and negative control (purple) .
anti-Death-Domain Associated Protein (DAXX) antibody (2) Figure 2: Confocal immunofluorescence analysis of PANC-1 cells using DAXX mouse mAb (green) . Blue: DRAQ5 fluorescent DNA dye.
anti-Death-Domain Associated Protein (DAXX) antibody (3) Figure 3: Confocal immunofluorescence analysis of Hela cells using DAXX mouse mAb (green) . Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
anti-Death-Domain Associated Protein (DAXX) antibody (4) Figure 1: Western blot analysis using DAXX mouse mAb against K562 cell lysate (1) .
Product cited in: Beausoleil, Jedrychowski, Schwartz et al.: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004 (PubMed).

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