Death-Domain Associated Protein (DAXX) (AA 558-740) antibody

Details for Product No. ABIN94302
Antigen
Epitope
AA 558-740
36
34
21
15
15
15
15
11
9
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
267
93
82
16
3
3
2
2
2
2
1
1
1
1
Host
Mouse
233
36
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
11
9
9
9
9
9
9
8
8
6
6
6
6
6
2
2
2
1
Application
Immunocytochemistry (ICC), Western Blotting (WB)
179
79
77
61
47
36
23
14
10
7
5
5
4
2
Options
Immunogen Recombinant C-terminal part (aa 558-740) of human Daxx.
Clone DAXX-01
Isotype IgG1
Specificity The antibody DAXX-01 reacts with Daxx, a death domain containing protein mainly expressed in fetal and adult human and mouse tissue.
Cross-Reactivity (Details) Human
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name Daxx (DAXX Antibody Abstract)
Background Daxx is an apoptosis-modulating death domain-associated protein with functions in transcriptional regulation. Daxx functions bot in cytoplasm, where it interacts with Fas, and in nucleus (residing in PML oncogenic domains), where it is involved in SUMO-dependent regulation of transcription and subnuclear compartmentalization. Daxx senzitizes the cells to apoptosis, but on the other hand, this protein may also serve in preventing apoptosis in the early embryo. Even regarding the transcription, Daxx can serve both as a corepressor and a coactivator. During ischemic stress, Daxx translocates from the nucleus to the cytoplasm, where in regulates sodium hydrogen exchanger isoform 1 (NHE1).
Pathways Intracellular Steroid Hormone Receptor Signaling Pathway
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Death-Domain Associated Protein (DAXX) (AA 558-740) antibody (ABIN94302) Immunofluorescence staining of Daxx in transfected HeLa human cervix carcinoma cell l...
Image no. 2 for anti-Death-Domain Associated Protein (DAXX) (AA 558-740) antibody (ABIN94302) Immunofluorescence staining of Daxx in transfected HeLa human cervix carcinoma cell l...
Product cited in: Leal-Sanchez, Couzinet, Rossin, Abdel-Sater, Chakrabandhu, Luci, Anjuere, Stebe, Hancock, Hueber: "Requirement for Daxx in mature T-cell proliferation and activation." in: Cell death and differentiation, Vol. 14, Issue 4, pp. 795-806, 2007 (PubMed).