Glutamate Dehydrogenase 1 (GLUD1) antibody

Antigen: Glutamate Dehydrogenase 1 (GLUD1)

Synonyms: GLUD1, GDH, MGC127177, Glud1, cb719, zgc:55630, wu:fb16e02, wu:fb58f12, wu:fe37f03, wu:fj43f02, glud1, MGC80801, gdh1, GDH1, GLUD, MGC132003, Glud, Gdh-X, Gludl, AI118167, MRG-2, MGC93608, GLUTAMATE DECARBOXYLASE 1, GLUTAMATE DEHYDROGENASE 1, MRG7.13, MRG7_13

Clonality: Polyclonal

Host: Rabbit

Reactivity: Cow (Bovine)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN99811

Additional Information

Alternative name: Glutamate Dehydrogenase

Gene ID: NP_872593

Swiss-Prot: P00366

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a full lengthGlutamate Dehydrogenase protein isolated from Bovine Liver.

Format: Lyophilized

Description: Glutamate is a major excitatory neurotransmitter. One enzyme central to the metabolism of glutamate is glutamate dehydrogenase (GDH1; EC 1.4.1.3), that catalyzes the reversible deamination of L-glutamate to 2-oxoglutarate using NAD+ or NADP+. Mammalian GDH is composed of six identical subunits, and the regulation of GDH is very complex. It has been a major goal to identify the substrate and regulatory binding sites of GDH. It is only in recent years that the three-dimensional structure of GDH from microorganisms is available. Very recently, crystallization of bovine liver GDH was reported for the first time from the mammalian sources. However, remarkably little is known about the detailed structure of mammalian GDH, especially the brain enzymes.

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Glutamate Dehydrogenase [BovineLiver]. BLAST analysis was used to determine that cross reactivity is suggested for both mitochondrial and brain isoforms (GDH1 andGDH2), from both bovine and human sources. Additionally similar reactivity is suggested for most primate species including greenmonkey, white gibbon, chimpanzee orangutan, and gorilla. A high degree of sequence homology is also noted for GDH from chicken,mouse, rat, dog, and other mammals as well as Xenopus tropicalis, zebrafish, rainbow trout and Atlantic salmon. Cross reactivityagainst Glutamate Dehydrogenase from other tissues and species may occur but have not been specifically determined.

Synonyms: GLUD1, GDH, MGC127177, Glud1, cb719, zgc:55630, wu:fb16e02, wu:fb58f12, wu:fe37f03, wu:fj43f02, glud1, MGC80801, gdh1, GDH1, GLUD, MGC132003, Glud, Gdh-X, Gludl, AI118167, MRG-2, MGC93608, GLUTAMATE DECARBOXYLASE 1, GLUTAMATE DEHYDROGENASE 1, MRG7.13, MRG7_13

Application Details

Application Notes: This antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivityshould be optimized by the end user. Bovine glutamate dehydrogenase exists as a homohexamer located within themitochondrial matrix. Expect a band approximately 62 kDa in size corresponding to glutamate dehydrogenase monomersubunit by western blotting in the appropriate cell or tissue extract. Dilutions: ELISA 1:4,000 - 1:16,000 WESTERN BLOT 1:1,000 - 1:3,000 IF MICROSCOPY User Optimized OTHER APPLICATIONS User Optimized

Concentration: 85.0 mg/ml (by Refractometry)

Purification: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Storage: Store vial at 4° C prior to restoration. Restore with 2.0 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

Research Area: Metabolism, Amino Acids, Enzymes

Restrictions: For Research Use only

Images

Western blot analysis is shown using antibodies-online's anti-Bovine Glutamate Dehydrogenase antibody to detect the enzyme from bovine liver preparations. Comparison to a molecular weight marker indicates a predominant band of ~62 kDa. The higher molecular weight band may represent a subunit dimer. A 4-20% gradient gel was used to separate proteins prior to transfer to 0.2 uM nitrocellulose. The blot was incubated with a 1:1,000 dilution of the antibody for 2 h at room temperature followed by detection using IRDye

Publications

Publications: Maechler, Gjinovci, Wollheim: "Implication of glutamate in the kinetics of insulin secretion in rat and mouse perfused pancreas." in: Diabetes, Vol. 51 Suppl 1, pp. S99-102, 2002 (PubMed).

Akutsu, Miyazaki: "Biochemical and immunohistochemical studies on tropomyosin and glutamate dehydrogenase in the chicken liver." in: Zoological science, Vol. 19, Issue 3, pp. 275-86, 2002 (PubMed).

Carobbio, Ishihara, Fernandez-Pascual et al.: "Insulin secretion profiles are modified by overexpression of glutamate dehydrogenase in pancreatic islets." in: Diabetologia, Vol. 47, Issue 2, pp. 266-76, 2004 (PubMed).

Fransen, Vastiau, Brees et al.: "Potential role for Pex19p in assembly of PTS-receptor docking complexes." in: The Journal of biological chemistry, Vol. 279, Issue 13, pp. 12615-24, 2004 (PubMed).

Korolainen, Goldsteins, Nyman et al.: "Oxidative modification of proteins in the frontal cortex of Alzheimer's disease brain." in: Neurobiology of aging, Vol. 27, Issue 1, pp. 42-53, 2005 (PubMed).

Huybrechts, Van Veldhoven, Hoffman et al.: "Identification of a novel PEX14 mutation in Zellweger syndrome." in: Journal of medical genetics, Vol. 45, Issue 6, pp. 376-83, 2008 (PubMed).

Anthonio, Brees, Baumgart-Vogt et al.: "Small G proteins in peroxisome biogenesis: the potential involvement of ADP-ribosylation factor 6." in: BMC cell biology, Vol. 10, pp. 58, 2009 (PubMed).