GLUD1 antibody

Catalog No. ABIN99811

Product Details anti-GLUD1 Antibody

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Reactivity: Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLUD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Characteristics: Concentration Definition: by Refractometry

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a full length Glutamate Dehydrogenase protein isolated from Bovine Liver.
Immunogentype:Native

Application Details

Application Notes: This antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Bovine glutamate dehydrogenase exists as a homohexamer located within the mitochondrial matrix.  Expect a band approximately 56 kDa in size corresponding to glutamate dehydrogenase monomer subunit by western blotting in the appropriate cell or tissue extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Concentration: 85 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-GLUD1 antibody (ABIN99811)

Pougovkina, te Brinke, Ofman, van Cruchten, Kulik, Wanders, Houten, de Boer: "Mitochondrial protein acetylation is driven by acetyl-CoA from fatty acid oxidation." in: Human molecular genetics, Vol. 23, Issue 13, pp. 3513-22, (2015) (PubMed).

Frigerio, Karaca, De Roo, Mlynárik, Skytt, Carobbio, Pajęcka, Waagepetersen, Gruetter, Muller, Maechler: "Deletion of Glud1 (Glutamate Dehydrogenase 1) in the Central Nervous System affects glutamate handling without altering synaptic transmission." in: Journal of neurochemistry, (2012) (PubMed).

Anthonio, Brees, Baumgart-Vogt, Hongu, Huybrechts, Van Dijck, Mannaerts, Kanaho, Van Veldhoven, Fransen: "Small G proteins in peroxisome biogenesis: the potential involvement of ADP-ribosylation factor 6." in: BMC cell biology, Vol. 10, pp. 58, (2009) (PubMed).

Huybrechts, Van Veldhoven, Hoffman, Zeevaert, de Vos, Demaerel, Brams, Jaeken, Fransen, Cassiman: "Identification of a novel PEX14 mutation in Zellweger syndrome." in: Journal of medical genetics, Vol. 45, Issue 6, pp. 376-83, (2008) (PubMed).

Korolainen, Goldsteins, Nyman, Alafuzoff, Koistinaho, Pirttilae: "Oxidative modification of proteins in the frontal cortex of Alzheimer's disease brain." in: Neurobiology of aging, Vol. 27, Issue 1, pp. 42-53, (2005) (PubMed).

Carobbio, Ishihara, Fernandez-Pascual, Bartley, Martin-Del-Rio, Maechler: "Insulin secretion profiles are modified by overexpression of glutamate dehydrogenase in pancreatic islets." in: Diabetologia, Vol. 47, Issue 2, pp. 266-76, (2004) (PubMed).

Fransen, Vastiau, Brees, Brys, Mannaerts, Van Veldhoven: "Potential role for Pex19p in assembly of PTS-receptor docking complexes." in: The Journal of biological chemistry, Vol. 279, Issue 13, pp. 12615-24, (2004) (PubMed).

Akutsu, Miyazaki: "Biochemical and immunohistochemical studies on tropomyosin and glutamate dehydrogenase in the chicken liver." in: Zoological science, Vol. 19, Issue 3, pp. 275-86, (2002) (PubMed).

Target Details for GLUD1

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Alternative Name: Glutamate Dehydrogenase (GLUD1 Products)

Synonyms: GDH antibody, gdh1 antibody, GDH 1 antibody, glud1 antibody, GLUD1 antibody, cb719 antibody, wu:fb16e02 antibody, wu:fb58f12 antibody, wu:fe37f03 antibody, wu:fj43f02 antibody, zgc:192851 antibody, zgc:55630 antibody, GDH1 antibody, GLUD antibody, AI118167 antibody, Gdh-X antibody, Glud antibody, Gludl antibody, Ac2-281 antibody, Gdh1 antibody, Gludeha antibody, MRG-2 antibody, GLUTAMATE DECARBOXYLASE 1 antibody, GLUTAMATE DEHYDROGENASE 1 antibody, MRG7.13 antibody, MRG7_13 antibody, glutamate dehydrogenase 1 antibody, C2H1orf130 antibody, legdh1 antibody, cb622 antibody, wu:fc33g09 antibody, wu:fc66a10 antibody, zgc:77186 antibody, glutamate dehydrogenase 1 antibody, glutamate dehydrogenase 1 S homeolog antibody, glutamate dehydrogenase 1, mitochondrial antibody, glutamate dehydrogenase 1b antibody, glutamate dehydrogenase antibody, non-compact myelin associated protein antibody, glutamate dehydrogenase 1a antibody, GLUD1 antibody, GDH1 antibody, glud1 antibody, glud1.S antibody, LOC693461 antibody, glud1b antibody, Glud1 antibody, HACJB3_RS00320 antibody, LOC100587725 antibody, NCMAP antibody, gdh1 antibody, glud1a antibody

Background: Glutamate is a major excitatory neurotransmitter. One enzyme central to the metabolism of glutamate is glutamate dehydrogenase (GDH1; EC 1.4.1.3), that catalyzes the reversible deamination of L-glutamate to 2-oxoglutarate using NAD+ or NADP+. Mammalian GDH is composed of six identical subunits, and the regulation of GDH is very complex.  It has been a major goal to identify the substrate and regulatory binding sites of GDH. It is only in recent years that the three-dimensional structure of GDH from microorganisms is available. Very recently, crystallization of bovine liver GDH was reported for the first time from the mammalian sources. However, remarkably little is known about the detailed structure of mammalian GDH, especially the brain enzymes.
Synonyms: Glutamate dehydrogenase 1, mitochondrial GDH 1 EC=1.4.1.3

Gene ID: 281785, 32880221

UniProt: P00366

Pathways: Positive Regulation of Peptide Hormone Secretion, Warburg Effect