EpCAM antibody

Catalog No. ABIN349703

The Mouse Monoclonal anti-EpCAM antibody has been validated for WB, FACS, IHC (p), IP and ICC. It is suitable to detect EpCAM in samples from Human. There are 6+ publications available.

Quick Overview for EpCAM antibody (ABIN349703)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EpCAM antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 323-A3

Product Details anti-EpCAM Antibody

Purpose: Anti-Hu CD326 Purified

Specificity: The mouse monoclonal antibody 323/A3 recognizes an extracellular epitope of CD326 / EpCAM, a marker of epithelial lineages, that is over-expressed in many carcinomas.

No Cross-Reactivity: Rat

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Human breast cancer MCF-7 cells

Isotype: IgG1

Application Details

Application Notes: Western blotting: Non-reducing conditions, recommended dilution: 1-2 μg/mL, positive control: MCF-7 cells.
Immunoprecipitation: Recommended dilution: 1-4 μg / ml per 100-500 μg of protein in 1 mL lysate.
Immunohistochemistry (paraffin sections): Recommended dilution: 1-10 μg/mL, pretreatment: tissue section digestion with pepsin (15 min at RT or 10 min at 37 °C, 1 mg / ml Tris-HCl, pH 2.0), positive tissue: breast carcinoma.
Immunocytochemistry: Recommended dilution: 1-10 μg/mL.
Flow cytometry: Recommended dilution: 1-4 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-EpCAM antibody (ABIN349703)

Kirman, Jenkins, Fowler, Whelan: "Naturally occurring antibodies to epithelial cell adhesion molecule (EpCAM)." in: Digestive diseases and sciences, Vol. 48, Issue 12, pp. 2306-9, (2004) (PubMed).

Winter, Nagtegaal, van Krieken, Litvinov: "The epithelial cell adhesion molecule (Ep-CAM) as a morphoregulatory molecule is a tool in surgical pathology." in: The American journal of pathology, Vol. 163, Issue 6, pp. 2139-48, (2003) (PubMed).

Balzar, Briaire-de Bruijn, Rees-Bakker, Prins, Helfrich, de Leij, Riethmüller, Alberti, Warnaar, Fleuren, Litvinov: "Epidermal growth factor-like repeats mediate lateral and reciprocal interactions of Ep-CAM molecules in homophilic adhesions." in: Molecular and cellular biology, Vol. 21, Issue 7, pp. 2570-80, (2001) (PubMed).

Balzar, Bakker, Briaire-de-Bruijn, Fleuren, Warnaar, Litvinov: "Cytoplasmic tail regulates the intercellular adhesion function of the epithelial cell adhesion molecule." in: Molecular and cellular biology, Vol. 18, Issue 8, pp. 4833-43, (1998) (PubMed).

Litvinov, Bakker, Gourevitch, Velders, Warnaar: "Evidence for a role of the epithelial glycoprotein 40 (Ep-CAM) in epithelial cell-cell adhesion." in: Cell adhesion and communication, Vol. 2, Issue 5, pp. 417-28, (1995) (PubMed).

Litvinov, Velders, Bakker, Fleuren, Warnaar: "Ep-CAM: a human epithelial antigen is a homophilic cell-cell adhesion molecule." in: The Journal of cell biology, Vol. 125, Issue 2, pp. 437-46, (1994) (PubMed).

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: CD326

Background: Epithelial cell adhesion molecule,CD326 / EpCAM (also known as ESA, EGP40, EGP-2, KSA1/4, CO17-1A, GA733-2, MOC31, Ber-EP4) is a 40 kDa transmembrane glycoprotein serving as adhesion molecule in the basolateral membranes in a variety of epithelial cells. CD326 mediates calcium-independent homotypic cell-cell adhesions. CD326 over-expression has been detected in many epithelial tumours and is often associated with bad prognosis. It has been used for diagnostics of (pre-) malignancies at early stages.,EPCAM, GA733-2, EGP314, KSA, KS 1/4 antigen, Trop-1, M4S1, DIAR5, MIC18, TROP1

Gene ID: 4072

UniProt: P16422