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Rat (Rattus) ICOS ELISA Kit

Recommended ICOS Product (supplied by: Log in to see )

Antigen
Inducible T-Cell Co-Stimulator (ICOS) ELISA Kits
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  • Ailim
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  • ICOS
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  • Icos
Reactivity
Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
3
3
2
1
1
1
1
1
1
1
Method Type
Sandwich ELISA
Detection Range
78.125-5000 pg/mL
Minimum Detection Limit
78.125 pg/mL
Application
ELISA
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Catalog No. ABIN4993507
$ 638.00
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
1 ABIN1503259 Serum, Plasma, Cell Culture Supernatant, Body Fluids, Tissue Homogenate Log in to see
1 ABIN2948468 78.125-5000 pg/mL 78.125 pg/mL Log in to see
1 ABIN5073050 Sandwich ELISA Plasma, Serum, Tissue Homogenate, Biological Fluids Log in to see

Similar ICOS ELISA Kits

Application / Reactivity Rat (Rattus)
ELISA 4 ELISA Kits

General

Antigen Inducible T-Cell Co-Stimulator (ICOS) ELISA Kits
Reactivity Rat (Rattus)
Kits with alternative reactivity to:
(3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Method Type Sandwich ELISA
Detection Range 78.125-5000 pg/mL
Minimum Detection Limit 78.125 pg/mL
Application ELISA
Supplier Log in to see

Product Details ICOS ELISA Kit

Target details Application Details Handling Images
Purpose The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ICOS in Rat serum, plasma and other biological fluids
Sample Type Plasma, Serum, Biological Fluids
Detection Method Colorimetric
Analytical Method Quantitative
Specificity This kit recognizes natural and recombinantRatICOS. No significant cross-reactivity or interference between RatICOS and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between RatICOS and all the analogues.
Sensitivity 46.875 pg/mL
Components Micro ELISA Plate
Reference Standard
Concentrated Biotinylated Detection Ab
Concentrated HRP Conjugate
Reference Standard & Sample Diluent
Biotinylated Detection Ab Diluent
HRP Conjugate Diluent
Concentrated Wash Buffer (25×)
Substrate Reagent
Stop Solutio
Plate Sealer
Product Description
Certificate of Analysis
Material not included Microplate reader with 450nm wavelength filter
High-precision transferpettor, EP tubes and disposable pipette tips
37℃ Incubator
Deionized or distilled water
Absorbent paper
Loading slot for Wash Buffer

Target details

Product Details ICOS ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name Inducible T-cell costimulator (ICOS) (ICOS ELISA Kit Abstract)
Pathways TCR Signaling

Application Details

Product Details ICOS ELISA Kit Target details Handling Images back to top
Comment

Information on standard material:
The formulation of the standard is 0.01 M PBS. The standard contains additives (1 % BSA).

Information on reagents:
Reagents include 1 M SO2. Azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials are not used.

Information on antibodies:
The provided antibodies and their host vary in different kits. All antibodies are affinity purified

The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

Sample Volume 100 µL
Plate Pre-coated,Strips (12 x 8)
Protocol This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ICOS. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ICOSand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ICOS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ICOS.You can calculate the concentration of ICOSin the samples by comparing the OD of the samples to the standard curve. 6th Edition, revised in June, 2015 www.elabscience.com 4
Restrictions For Research Use only

Handling

Product Details ICOS ELISA Kit Target details Application Details Images back to top
Handling Advice All the reagents in the kit should be stored according to the labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20 °C (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination. If not to store reagents according to above suggestions, erroneous results may occur.
Storage 4 °C/-20 °C
Storage Comment The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.