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CD82 antibody (CD82 Molecule)

Details for Product anti-CD82 Antibody No. ABIN1043024, Supplier: Log in to see
Antigen
  • AA682076
  • AL023070
  • C33
  • IA4
  • Kai1
  • Tspan27
  • 4F9
  • GR15
  • KAI1
  • R2
  • SAR2
  • ST6
  • TSPAN27
Alternatives
anti-Human CD82 antibody for Flow Cytometry
Reactivity
Human
121
45
31
1
Host
Mouse
96
38
Clonality (Clone)
Monoclonal ()
Conjugate
This CD82 antibody is un-conjugated
10
9
8
6
4
2
2
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
85
48
43
41
25
18
13
12
12
8
4
2
1
1
1
1
Supplier
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Immunogen C91/PL (human HTLV-1+ T cell line)
Clone C33
Isotype IgG2a
Specificity The antibody C33 reacts with CD82, a widely expressed cell surface protein of the tetraspanin family. CD82 is also found in endosome/lysosome compartments.
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Alternative Name CD82 (CD82 Antibody Abstract)
Background CD82 (KAI1), a member of the tetraspanin family, forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules. These complexes influence adhesion, morphology, activation, proliferation and differentiation of B, T and other cells. CD82 regulates cytoskeleton rearrangement and may participate in the turnover of the tetraspanin complex members. Besides in the plasma membrane, CD82 is localized also in endosome/lysosome compartments. Tumour-suppressive roles of CD82 have been demonstrated.
Gene ID 3732
Research Area Cancer, Transcription Factors, CD Antigens, Adaptive Immunity, Surface Receptors of Immune Cells
Pathways p53 Signaling
Application Notes Functional Application: In human MOLT-4 T-cell line the antibody C33 inhibits syncytium formation induced by coculture with human T-cell leukemia virus type 1 (HTLV-1)-positive human T-cell lines.

Working concentrations should be determined by the investigator.
Comment

CD and Related Antigens (Human)

Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Product cited in: Schatzlmaier, Supper, Göschl et al.: "Rapid multiplex analysis of lipid raft components with single-cell resolution." in: Science signaling, Vol. 8, Issue 395, pp. rs11, 2015 (PubMed).

Escola, Kleijmeer, Stoorvogel et al.: "Selective enrichment of tetraspan proteins on the internal vesicles of multivesicular endosomes and on exosomes secreted by human B-lymphocytes." in: The Journal of biological chemistry, Vol. 273, Issue 32, pp. 20121-7, 1998 (PubMed).

Ueda, Ichikawa, Tamaru et al.: "Expression of the KAI1 protein in benign prostatic hyperplasia and prostate cancer." in: The American journal of pathology, Vol. 149, Issue 5, pp. 1435-40, 1996 (PubMed).

Imai, Yoshie et al.: "C33 antigen and M38 antigen recognized by monoclonal antibodies inhibitory to syncytium formation by human T cell leukemia virus type 1 are both members of the transmembrane 4 superfamily and ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 11, pp. 6470-81, 1994 (PubMed).

Imai, Kakizaki, Nishimura et al.: "Molecular analyses of the association of CD4 with two members of the transmembrane 4 superfamily, CD81 and CD82." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 3, pp. 1229-39, 1995 (PubMed).

Fukudome, Furuse, Imai et al.: "Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T-cell leukemia virus type 1 (HTLV-1)-induced syncytium formation: altered glycosylation of C33 antigen ..." in: Journal of virology, Vol. 66, Issue 3, pp. 1394-401, 1992 (PubMed).