Transferrin Receptor antibody (APC)

Catalog No. ABIN1112224

Product Details anti-Transferrin Receptor Antibody (APC)

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Transferrin Receptor antibody is conjugated to APC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Characteristics: Monoclonal Mouse Anti-Human CD71.

Clone: FG2-12

Isotype: IgG1

Application Details

Application Notes: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 20 µl/10^6 cells.

Comment:

Allophycocyanin (Febico, Far East Bio-Tech Co.).

Sample Collection: 1. Transfer20 µl of anticoagulated (EDTA) BM to a 12 x 75 mm polystyrene test tube (10^6 cells). 2. Add 20 µl of CD71 APC and mix gently with a vortex mixer. The 20 µl is a guideline only, the optimal volume should be determined by the individual laboratory. 3. The recommended negative control is a non-reactive APC-conjugated antibody of the same isotype. 4. Incubate in the dark at room temperature at 4°C for 30 minutes or at room temperature (20-25 °C) for 15 minutes. 5. Add 1,5 ml of Lysing Solution to each sample and mix gently with a vortex mixer. Incubate for 10 minutes at room temperature in the dark. 6. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 7. Add 2 ml 0.01 mol/l PBS (It betters that it containing 2% bovine serum albumin) and resuspend the cells by using a vortex mixer. 8. Centrifuge at 1000 x g for 5 minutes. Gently aspirate the supernatant and discard it leaving approximately 50 µl of fluid. 9. Resuspend pellet in an appropriate fluid for flow cytometry, e.g. 0.3 ml PBS. The PBS should contain 1% paraformaldehyde (fixative) if samples are not analysed the same day. 10. Analyse on a flow cytometer or store at 2-8 °C in the dark until analysis. Samples can be run up to 24 hours after lysis. Normal Peripheral Blood on a human donor.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA) and 0,09% Sodium azide, pH 7.2.

Preservative: Sodium azide

Precaution of Use: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Storage: 4 °C

Target Details for Transferrin Receptor

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Alternative Name: CD71 (TFRC Products)

Background: Human Transferrin Receptor which is expressed on proliferating lymphoblasts, majority human tumour cell lines, normal peripheral blood monocytes. The CD71 antibody in routine is used along with the glycophorin A to develop a flow-cytometric approach to the evaluation of erythroid and myeloid dysplasia in patients with Myelodysplatic Syndromes (MDS). Another of the applications of the CD71 antibody, is the recount of retyculocites2, immature red corpuscles, without nucleus, that contain ribonucleic acid (RNA). peripheral blood, they delay approximately a day in losing their RNA and to be become erythrocytes. Its recount provides information on the production of erythrocytes.

Pathways: Transition Metal Ion Homeostasis