Complement C4d antibody

Details for Product No. ABIN111802
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(5), (4)
Clonality (Clone)
Monoclonal ()
Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(6), (5), (1), (1), (1), (1)
Pubmed 7 references available
Catalog no. ABIN111802
Quantity 0.1 mg
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Immunogen Native human C4 from plasma
Clone 10-11
Isotype IgG1
Specificity This antibody recognises the secreted protein complement component 4d (C4d).
Purification Affinity chromatography on Protein A.
Background The complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4bs alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonisation and activation of the classical pathway. This requires C4 binding protein or CR1 as a cofactor. iC4b is further degraded into C4d and C4c. C4ds short half life means that C4d is present in serum at high enough concentrations to make it a useful marker for classical complement activation. C4 has been linked to susceptibility to systemic lupus erythematosous.
Alternate names: Acidic complement C4, Basic complement C4, CPAMD2, CPAMD3, Complement component 4
Gene ID 720
NCBI Accession NP_009224
UniProt P0C0L4
Application Notes Immunohistochemistry on Frozen Sections (1/100-1/750): works very well on acetone-fixed,frozen renal biopsies, Strong staining is observed in the glomeruli and in some cases theperitubular capillaries. Immunohistochemistry on Paraffin Sections: variable result on formalin-fixed,paraffin-embedded sections, It has been observed that pre-treatment with 88% formic acidfor 20 minutes at room temperature is beneficial. ELISA (1/5000-1/20000). Western Blot. Immunofluorescence (1/250-1/600). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, pH 8.4 containing 0.09% Sodium Azide as preservative.
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Rogers, Cooper, Webster et al.: "Complement activation by beta-amyloid in Alzheimer disease." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 21, pp. 10016-20, 1992 (PubMed).

Collins, Schneeberger, Pascual et al.: "Complement activation in acute humoral renal allograft rejection: diagnostic significance of C4d deposits in peritubular capillaries." in: Journal of the American Society of Nephrology : JASN, Vol. 10, Issue 10, pp. 2208-14, 1999 (PubMed).

Stoltzner, Grenfell, Mori et al.: "Temporal accrual of complement proteins in amyloid plaques in Down's syndrome with Alzheimer's disease." in: The American journal of pathology, Vol. 156, Issue 2, pp. 489-99, 2000 (PubMed).

Mauiyyedi, Pelle, Saidman et al.: "Chronic humoral rejection: identification of antibody-mediated chronic renal allograft rejection by C4d deposits in peritubular capillaries." in: Journal of the American Society of Nephrology : JASN, Vol. 12, Issue 3, pp. 574-82, 2001 (PubMed).

Mauiyyedi, Crespo, Collins et al.: "Acute humoral rejection in kidney transplantation: II. Morphology, immunopathology, and pathologic classification." in: Journal of the American Society of Nephrology : JASN, Vol. 13, Issue 3, pp. 779-87, 2002 (PubMed).

Knechtle, Pirsch, H Fechner et al.: "Campath-1H induction plus rapamycin monotherapy for renal transplantation: results of a pilot study." in: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, Vol. 3, Issue 6, pp. 722-30, 2003 (PubMed).

Xu, Melle, Golemanov et al.: "Shape and buckling transitions in solid-stabilized drops." in: Langmuir : the ACS journal of surfaces and colloids, Vol. 21, Issue 22, pp. 10016-20, 2005 (PubMed).

Hosts (5), (4)
Reactivities (9)
Applications (6), (5), (1), (1), (1), (1)
Epitopes (3), (1)
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