Complement C4A/C4B antibody
Reactivity: Human
WB, EIA, IHC (fro), FACS
Host: Mouse
Monoclonal
10-12-14
unconjugated
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- Target
- Complement C4A/C4B
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
- Specificity
- This antibody recognises complement component 4c (C4c).
- Characteristics
- Synonyms: Complement Component 4, CPAMD2, CPAMD3
- Purification
- Affinity chromatography on Protein A
- Immunogen
- Purified human C4c. Genename: C4c
- Clone
- 10-12-14
- Isotype
- IgG1
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anti-Complement C4A/C4B (Internal Region) antibody
Reactivity: Human WB, ELISA, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-Complement C4A/C4B (Center) antibodyReactivity: Human, Mouse, Rat WB Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Western Blot. Flow Cytometry. ELISA: This antibody binds both C4c and C4 and so is not suitable for all ELISAapplications. Immunohistochemistry on frozen sections. Recommended Postive Control: Kidney sections from post-streptococal glomerulonephritispastients.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0mg/mL
- Buffer
- Borate buffered saline, pH 8.4 containing 0.02 % Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C/-20 °C
- Storage Comment
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - Expiry Date
- 12 months
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- Target
- Complement C4A/C4B
- Synonyms
- C4 antibody, C4-1 antibody, C4b antibody, complement component 4A (Rodgers blood group) antibody, C4a antibody
- Background
- The complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4bs alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonisation and activation of the classical pathway. iC4b is further degraded into the inactive fragments C4d and C4c. C4c consists of two a-chain fragments, alpha 4 (13 kDa) and alpha 3 (25 kDa) linked to a beta chain through disulphide bridges and a gamma chain. It is expressed in the blood plasma.Synonyms: CPAMD2, CPAMD3, Complement Component 4
- Gene ID
- 720
- UniProt
- P0C0L4
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