Complement C4A/C4B (AA 721-770) antibody

Catalog No. ABIN6579207

This Rabbit Polyclonal antibody specifically detects Complement C4A/C4B in WB, ELISA, IF and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Complement C4A/C4B (AA 721-770) antibody (ABIN6579207)

Target: Complement C4A/C4B

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details

Binding Specificity: AA 721-770

Purpose: C4a/b Antibody

Specificity: C4a/b Polyclonal Antibody detects endogenous levels of C4a/b protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized peptide derived from the Internal region of human C4A/C4B. AA range:721-770

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC: 1:100-1:300. ELISA: 1:10000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details

Target: Complement C4A/C4B

Alternative Name: C4A/B

Background:

Synonyms: C4A, CO4, CPAMD2, Complement C4-A, Acidic complement C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2, C4B, CO4, CPAMD3, Complement C4-B, Basic complement C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3

Background: This gene encodes the acidic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus and type I diabetes mellitus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. Two transcript variants encoding different isoforms have been found for this gene. [provided

Gene Name: C4A/C4B

Protein Name: Complement C4-A/Complement C4-B

Molecular Weight: '192 kDa

Gene ID: 720, 721

OMIM: 120810

UniProt: P0C0L4