CD93 antibody (Biotin)

Catalog No. ABIN111896

Product Details anti-CD93 Antibody (Biotin)

Target: CD93

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD93 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This Monoclonal antibody clone X-2 recognizes a 110 kDa O-sialoglycoprotein on granulocytes, monocytes and endothelial cells. Upon differentiation of cultivated monocytes into macrophages, CDw93 ceases to be expressed on day 5-6, which makes it a very useful marker to differentiate those two cell populations. Antigen Distribution Isolated Cells: Positive with all granulocytes, with staining increasing transiently upon maturation. Antigen expression is increased by TNFα, GM-CSF, (fMLP) treatment. No effect on superoxide production by binding to neutrophils has been noted. PMA stimulation causes down regulation of the antigen. Positive with monocytes following fMLP, TNF, GM-CSF treatment. Antigen expression decreases on cultured monocytes. Positive on AML cases with monocytic component. Positive on cell lines HL-60, Namalva, K562, THP-1, U937, negative on HMC-1 (human mast cell line). Tissue Sections: The Monoclonal antibody X-2 stains epitheloid cells and Rosai-Dorfman cells and most endothelial cells in the synovial membrane of RA patients.

Purification: Affinity Chromatography

Immunogen: Human monocytes. Remarks: The antigen has been assigned to the CDw93 cluster, the epitope has not been further characterized.

Clone: X-2

Isotype: IgG1

Application Details

Application Notes: Suitable for Immunohistochemistry (IHC) on: Frozen sections: 0.5 μg/mL (1/400)Paraffin sections: does not react on routinely processedparaffin sections. It works alos in FACS. Suggested positive control: human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2, 0.01 % Thimerosal, 10 mg/mL BSA

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody at 2-8 °C before reconstitution. After reconstitution store at 2-8° for up to 2 months. DO NOT FREEZE. Recontstituted product is stable for 2 days at RT.

References for anti-CD93 antibody (Biotin) (ABIN111896)

Sumide, Matsuoka, Kawamura, Nakatsuka, Fujioka, Asano, Takihara, Sonoda: "A revised road map for the commitment of human cord blood CD34-negative hematopoietic stem cells." in: Nature communications, Vol. 9, Issue 1, pp. 2202, (2018) (PubMed).

Target Details for CD93

Target: CD93

Alternative Name: CD93 / C1QR1 (CD93 Products)

Background: CD93 a cell-surface glycoprotein and type I membrane protein involve in intercellular adhesion and in the clearance of apoptotic cells. The intracellular cytoplasmic tail of this protein has been found to interact with moesin, a protein known to play a role in linking transmembrane proteins to the cytoskeleton and in the remodelling of the cytoskeleton.Synonyms: C1q receptor, C1q/MBL/SPA receptor, CDw93, Complement component 1 q subcomponent receptor 1, Complement component C1q receptor, MBL receptor, MXRA4, Matrix-remodeling-associated protein 4, SPA receptor

Gene ID: 22918

NCBI Accession: NP_036204

UniProt: Q9NPY3