Intercellular Adhesion Molecule 1 (ICAM1) antibody

Antigen: Intercellular Adhesion Molecule 1 (ICAM1)

Synonyms: BB2, CD54, P3.58, Ly-47, Icam-1, MALA-2, MGC6195, ICAM-1, ICAM1, ICAM

Clonality: Monoclonal (1304)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN111934

Additional Information

Alternative name: CD54 / ICAM1

UniProt: P05362

Immunogen: Cultured human endothelial cells

Cross-Reactivity: Human

Isotype: IgG2a

Clone: 1304

Description: ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with anextracellular domain of five immunoglobulin superfamily repeats, a transmembrane regionand a cytoplasmic domain. It shares considerable amino acid sequence homology withICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected oncells of many other lineages (e. g. epithelial cells, fibroblasts, chondrocytes, Blymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells andneutrophils), with lower levels that increase in inflammation. ICAM1 is also detected insome carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and iselevated in patients with various inflammatory syndromes. It is the receptor forrhinoviruses and malaria.

Characteristics: Synonyms: ICAM-1, Intercellular adhesion molecule 1, Major Group Rhinovirus Receptor

Application Details

Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalumProtocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature1. Deparaffinize and rehydrate tissue section2. Incubate the tissue section with proteinase K for 7min. 3. Wash in distilled water4. Block endogenous peroxidase5. Wash in PBS

Application Notes: Suitable for Immunohistochemistry (IHC), FACS and Western Blots. Recommended Dilutions for IHC: Frozen sections: 0.25 ug/ml (1/800). Paraffin sections: 2 ug/ml (1/100), Proteinase K pretreatment for antigen retrieval isrecommended. Suggested positive control: Human tonsil. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.2 mg/ml (following reconstitution)

Purification: Purified

Buffer: PBS pH 7.2 with 10 mg/ml bovine serum albumin (BSA) as a stabilizer and0.01 % thimerosal as a preservativeReconstitution: Restore with 0.5 ml distilled water.

Storage: Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Do not freeze working dilutions. Avoid repeated freezing and thawing. Shelf life: One year from despatch.

Restrictions: For Research Use only

Publications

Springer: "Adhesion receptors of the immune system." in: Nature, Vol. 346, Issue 6283, pp. 425-34, 1990 (PubMed).

Freudenthal, Steinman: "The distinct surface of human blood dendritic cells, as observed after an improved isolation method." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 19, pp. 7698-702, 1990 (PubMed).

Albelda, Buck: "Integrins and other cell adhesion molecules." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 4, Issue 11, pp. 2868-80, 1990 (PubMed).