Keratin 8 (KRT8) antibody

Details for Product No. ABIN112171
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Antigen
Synonyms
CARD2, CK-8, CK8, CYK8, K2C8, K8, KO, AA960620, AL022697, AU019895, Card2, EndoA, Krt-2.8, Krt2-8, CYKER, KRT2-8, KERATIN8, DreK8, cb186, krt2-8, sb:cb186, wu:fa20h05, wu:fa95h10, wu:fb96c06, zf-K8, z ... show more
CARD2, CK-8, CK8, CYK8, K2C8, K8, KO, AA960620, AL022697, AU019895, Card2, EndoA, Krt-2.8, Krt2-8, CYKER, KRT2-8, KERATIN8, DreK8, cb186, krt2-8, sb:cb186, wu:fa20h05, wu:fa95h10, wu:fb96c06, zf-K8, zfk8, ck8, cyk8, k2c8, card2, krt2-5, MGC69490, KRT8, cb59, etID20321.6, krt8, wu:fa91a11, wu:fd19e09, wu:fl23c09, DKFZp468F2127 show less
Reactivity
Hamster, Human, Mouse (Murine), Rat (Rattus)
(349), (100), (76), (24), (16), (14), (13), (9), (9), (8), (7), (4), (3), (2), (1), (1), (1), (1)
Host
Mouse
(217), (155), (4), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(17), (9), (5), (4), (4), (4), (4), (4), (4), (4), (4), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(241), (165), (102), (97), (80), (67), (61), (40), (29), (17), (6), (6), (3), (1), (1)
Pubmed 5 references available
Catalog no. ABIN112171
Quantity 50 µg
Price
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Immunogen Cytoskeletal proteins from cultured HeLa cells
Clone Ks8-7
Isotype IgG1
Specificity Ks 8.7 represents an excellent marker to discriminate simple epithelia from those of different origin. Polypetide reacting: Mr 52500 polypeptide (keratin K8, formerly also designated cytokeratin 8) of human epithelia. Tumors specifically detected: All adeno-CA tested, undifferentiated CA, cervix CA, hepatocellular CA. Reactivities on cultured cell lines (tested so far): MCF-7, RT 112, PLC.
Purification Protein A affinity chromatography
Alternative Name Cytokeratin 8
Background Cytokeratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with cytokeratin 18. Cytokeratin 8 is primarily found in the non squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18.
Alternate names: CK8, CYK8, Cytokeratin endo A, Cytokeratin-8, K8, KRT8, Keratin, Keratin-8, type II cytoskeletal 8
Gene ID 3856
NCBI Accession NP_002264.1
UniProt P05787
Research Area Cancer, Cytoskeleton, Cytokeratins, Extracellular Matrix
Application Notes Western blot. Immunocytology. Immunohistochemistry (1: 10) on frozen tissues and paraffin-embedded sections aftermicrowave treatment. Incubation time: 1 h at RT. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore in 1 mL distilled water.
Buffer Final solution contains 0.09 % sodium azide and 0.5% BSA in PBS buffer, pH 7.4.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Bártek, Vojtĕsek, Stasková et al.: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, 1991 (PubMed).

Osborn, van Lessen, Weber et al.: "Differential diagnosis of gastrointestinal carcinomas by using monoclonal antibodies specific for individual keratin polypeptides." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 55, Issue 4, pp. 497-504, 1986 (PubMed).

Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).

Gomi, Yokoyama, Fujimoto et al.: "Mice devoid of the glial fibrillary acidic protein develop normally and are susceptible to scrapie prions." in: Neuron, Vol. 14, Issue 1, pp. 29-41, 1995 (PubMed).

Stumptner, Fuchsbichler, Heid et al.: "Mallory body--a disease-associated type of sequestosome." in: Hepatology (Baltimore, Md.), Vol. 35, Issue 5, pp. 1053-62, 2002 (PubMed).

Hosts (217), (155), (4), (3)
Reactivities (349), (100), (76), (24), (16), (14), (13), (9), (9), (8), (7), (4), (3), (2), (1), (1), (1), (1)
Applications (241), (165), (102), (97), (80), (67), (61), (40), (29), (17), (6), (6), (3), (1), (1)
Conjugates (17), (9), (5), (4), (4), (4), (4), (4), (4), (4), (4), (1), (1)
Epitopes (18), (18), (16), (15), (15), (13), (13), (10), (8), (6), (4), (4), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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