Keratin 8 (KRT8) antibody

Antigen: Keratin 8 (KRT8)

Synonyms: K8, KO, CK8, CYK8, K2C8, CARD2, Card2, EndoA, Krt2-8, Krt-2.8, AA960620, AL022697, AU019895, MGC118110, CYKER, KRT2-8, krt8, MGC53564, KRT8, ck8, cyk8, k2c8, card2, krt2-5, MGC69490, cb59, zf-K8, MGC76893, wu:fa91a11, wu:fd19e09, wu:fl23c09, etID20321.6, zfk8, DreK8, cb186, krt2-8, MGC85764, sb:cb186, MGC174782, wu:fa20h05, wu:fa95h10, wu:fb96c06, DKFZp468F2127

Clonality: Monoclonal (Ks8-7)

Host: Mouse

Reactivity: Hamster

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN112171

Additional Information

Alternative name: Cytokeratin 8

UniProt: P05787

Immunogen: Cytoskeletal proteins from cultured HeLa cells

Cross-Reactivity: Hamster, Human, Mouse (Murine), Rat (Rattus)

Isotype: IgG1

Clone: Ks8-7

Description: Cytokeratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins andexists in combination with cytokeratin 18. Cytokeratin 8 is primarily found in the nonsquamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by theuse of antibodies that recognize only cytokeratin polypeptides 8 and 18.

Characteristics: Synonyms: KRT8, CYK8, Cytokeratin-8, CK8, Keratin-8, K8, Cytokeratin endo A, Keratin, type IIcytoskeletal 8

Specificity: Ks 8. 7 represents an excellent marker to discriminate simple epithelia from those ofdifferent origin. Polypetide reacting: Mr 52500 polypeptide (keratin K8, formerly also designatedcytokeratin 8) of human epithelia. Tumors specifically detected: All adeno-CA tested, undifferentiated CA, cervix CA,hepatocellular CA. Reactivities on cultured cell lines (tested so far): MCF-7, RT 112, PLC. Species: Human, mouse, rat, Tupaia belangeri, Monodelphis domestica, hamster. Negative with bovine and pig. Others not tested.

Synonyms: K8, KO, CK8, CYK8, K2C8, CARD2, Card2, EndoA, Krt2-8, Krt-2.8, AA960620, AL022697, AU019895, MGC118110, CYKER, KRT2-8, krt8, MGC53564, KRT8, ck8, cyk8, k2c8, card2, krt2-5, MGC69490, cb59, zf-K8, MGC76893, wu:fa91a11, wu:fd19e09, wu:fl23c09, etID20321.6, zfk8, DreK8, cb186, krt2-8, MGC85764, sb:cb186, MGC174782, wu:fa20h05, wu:fa95h10, wu:fb96c06, DKFZp468F2127

Application Details

Application Notes: Western blot. Immunocytology. Immunohistochemistry (1: 10) on frozen tissues and paraffin-embedded sections aftermicrowave treatment. Incubation time: 1 h at RT. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Purified

Buffer: Final solution contains 0.09 % sodium azide and 0.5% BSA in PBS buffer,pH 7.4.Reconstitution: Restore in 1 ml distilled water.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Cancer, Cytoskeleton, Cytokeratins, Extracellular Matrix

Restrictions: For Research Use only

Publications

Bártek, Vojtĕsek, Stasková et al.: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, 1991 (PubMed).

Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).

Gomi, Yokoyama, Fujimoto et al.: "Mice devoid of the glial fibrillary acidic protein develop normally and are susceptible to scrapie prions." in: Neuron, Vol. 14, Issue 1, pp. 29-41, 1995 (PubMed).

Stumptner, Fuchsbichler, Heid et al.: "Mallory body--a disease-associated type of sequestosome." in: Hepatology (Baltimore, Md.), Vol. 35, Issue 5, pp. 1053-62, 2002 (PubMed).