Keratin 8 (KRT8) antibody

Details for Product No. ABIN335327, Supplier: Log in to see
Antigen
  • CARD2
  • CK-8
  • CK8
  • CYK8
  • K2C8
  • K8
  • KO
  • AA960620
  • AL022697
  • AU019895
  • Card2
  • EndoA
  • Krt-2.8
  • Krt2-8
  • CYKER
  • KRT2-8
  • KERATIN8
  • ck8
  • cyk8
  • k2c8
  • card2
  • krt2-5
  • MGC69490
  • KRT8
  • DreK8
  • cb186
  • krt2-8
  • sb:cb186
  • wu:fa20h05
  • wu:fa95h10
  • wu:fb96c06
  • zf-K8
  • zfk8
  • DKFZp468F2127
  • keratin 8
  • keratin 8 S homeolog
  • KRT8
  • Krt8
  • krt8.S
  • krt8
Reactivity
Human, Mouse (Murine)
950
214
163
121
96
84
79
69
45
16
13
13
11
5
3
3
2
2
1
1
1
Host
Mouse
700
262
5
2
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
40
23
21
19
17
13
13
12
10
10
10
10
10
10
10
9
9
9
9
9
9
9
9
9
9
9
9
9
9
9
9
9
8
2
2
2
2
2
2
2
2
2
2
2
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC), Western Blotting (WB)
572
529
441
371
320
216
199
184
170
64
28
27
14
13
6
4
2
1
1
1
Options
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Supplier Product No.
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Immunogen M20 is a mouse monoclonal IgG1 antibody derived by fusion of murine myeloma cells with spleen cells from a mouse immunized with keratin isolated from the human breast carcinoma cell line MCF-7.
Clone M20
Isotype IgG1
Specificity Human and mouse.
Purification Purified
Antigen
Alternative Name Cytokeratin 8 / Keratin K8 (KRT8 Antibody Abstract)
Background Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual cytokeratin polypeptides are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
Application Notes M20 reacts with glandular epithelial cells (endocrine and exocrine) as well as mesothelial cells of the digestive, respiratory and urogenital tract and most adenocarcinomas derived from these cells. M20 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen tissues and flow cytometry. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
Restrictions For Research Use only
Storage 4 °C
Supplier Images
 image for anti-Keratin 8 (KRT8) antibody (ABIN335327) anti-Keratin 8 (KRT8) antibody
 image for anti-Keratin 8 (KRT8) antibody (ABIN335327) anti-Keratin 8 (KRT8) antibody (Image 2)
 image for anti-Keratin 8 (KRT8) antibody (ABIN335327) anti-Keratin 8 (KRT8) antibody (Image 3)
Immunohistochemistry (Frozen Sections) (IHC (fro)) image for anti-Keratin 8 (KRT8) antibody (ABIN335327) Immunohistochemistry on frozen section of human colon
Immunohistochemistry (Frozen Sections) (IHC (fro)) image for anti-Keratin 8 (KRT8) antibody (ABIN335327) Immunohistochemistry on frozen section of human colon
Product cited in: Waseem, Karsten, Leigh, Purkis, Waseem, Lane: "Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability." in: Biochemistry, Vol. 43, Issue 5, pp. 1283-95, 2004 (PubMed).

Ku, Gish, Wright, Omary: "Keratin 8 mutations in patients with cryptogenic liver disease." in: The New England journal of medicine, Vol. 344, Issue 21, pp. 1580-7, 2001 (PubMed).

Wetzels, Kuijpers, Lane, Leigh, Troyanovsky, Holland, van Haelst, Ramaekers: "Basal cell-specific and hyperproliferation-related keratins in human breast cancer." in: The American journal of pathology, Vol. 138, Issue 3, pp. 751-63, 1991 (PubMed).

Ramaekers, van Niekerk, Poels, Schaafsma, Huijsmans, Robben, Schaart, Vooijs: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." in: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, 1990 (PubMed).

Schaafsma, Ramaekers, van Muijen, Lane, Leigh, Robben, Huijsmans, Ooms, Ruiter: "Distribution of cytokeratin polypeptides in human transitional cell carcinomas, with special emphasis on changing expression patterns during tumor progression." in: The American journal of pathology, Vol. 136, Issue 2, pp. 329-43, 1990 (PubMed).

Smedts, Ramaekers, Robben, Pruszczynski, van Muijen, Lane, Leigh, Vooijs: "Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia." in: The American journal of pathology, Vol. 136, Issue 3, pp. 657-68, 1990 (PubMed).

Ivanyi, Groeneveld, Van Doornewaard, Mooi, Hageman: "Keratin subtypes in carcinomas of the uterine cervix: implications for histogenesis and differential diagnosis." in: Cancer research, Vol. 50, Issue 16, pp. 5143-52, 1990 (PubMed).

Schaafsma, Ramaekers, van Muijen, Ooms, Ruiter: "Distribution of cytokeratin polypeptides in epithelia of the adult human urinary tract." in: Histochemistry, Vol. 91, Issue 2, pp. 151-9, 1989 (PubMed).

Van Muijen, Ruiter, Warnaar: "Coexpression of intermediate filament polypeptides in human fetal and adult tissues." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 57, Issue 4, pp. 359-69, 1987 (PubMed).

Van Muijen, Warnaar, Ponec: "Differentiation-related changes of cytokeratin expression in cultured keratinocytes and in fetal, newborn, and adult epidermis." in: Experimental cell research, Vol. 171, Issue 2, pp. 331-45, 1987 (PubMed).