Integrin, alpha 4 (Antigen CD49D, alpha 4 Subunit of VLA-4 Receptor) (ITGA4) antibody (Biotin)

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Antigen
Synonyms CD49D, IA4, LOC100219853, CD11c
Reactivity
Mouse (Murine)
(156), (119), (37), (6), (5), (4), (1), (1), (1), (1), (1)
Host
Rat
(105), (104), (46)
Clonality (Clone)
Monoclonal ()
Conjugate
Biotin
(45), (28), (24), (8), (2), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
(198), (70), (45), (41), (36), (23), (22), (22), (19), (13), (5), (2), (2), (1), (1)
Pubmed 3 references available
Catalog no. ABIN114224
Quantity 0.1 mg
Price
253.00 $   Plus shipping costs $45.00
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Immunogen Peyers Patch HEV binding lymphoma line (TK1) Donor: Fisher Spleen Fusion Partner: P3x63Ag8.653
Clone R1-2
Isotype IgG2b
Specificity This monoclonal antibody reacts with a4 integrin, which helps to mediate cell-cell and cell- matrix interactions.
Purification Protein G Chromatography
Alternative Name CD49d / ITGA4
Background Integrin alpha 4 (also called CD49d) is a 150 kDa protein that possesses a large extracellular domain involved in ligand binding, a single transmembrane domain, and an intracellular regulatory domain possessing multiple sites for phosphorylation. Integrin alpha 4 forms heterodimers with integrins beta 1 and beta 7. Integrin alpha 4 is expressed on leukocytes and leukocyte precursors, neural crest cells, and developing skeletal muscles and is essential for embryogenesis, hematopoiesis, and immune responses. The presence of integrin alpha 4 promotes cell migration and inhibits cell spreading and contractility. Integrin alpha 4 function has been implicated in the pathogenesis of multiple diseases including asthma, rheumatoid arthritis, Crohn's disease, ulcerative colitis, hepatitis C, and multiple sclerosis, and therefore, modulation of integrin alpha 4 function has become an important target for drug discovery.
Alternate names: CD49 antigen-like family member D, Integrin alpha-4, Integrin alpha-IV, VLA-4, VLA4
Gene ID 16401
NCBI Accession NP_034706.3
UniProt Q00651
Research Area CD Antigens, Surface Receptors of Immune Cells, Cell/Tissue Markers, Extracellular Matrix, Signaling
Application Notes Flow cytometry. Immunoprecipitation. Immunohistochemistry. (1,2,3)Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add ~1. 0 µg* of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (Streptavidin-FITC) at a 1: 500 dilution. 9. Incubate tubes at 4°C for 30 - 60 minutes (It is recommended that tubes are protectedfrom light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Mouse Strain: BALB/cCell Concentration: 1x10e6 cells per testsAntibody Concentration Used: 1. 0 µg/10e6 cellsIsotypic Control: Biotin Rat IgG2bCell Source: Percentage of cells stained above control: TK1 Cells: 97. 9%Thymus: 80. 0%Spleen: 85. 1%Bone Marrow: 72. 4%
Restrictions For Research Use only
Format Liquid
Concentration 0.1 mg/mL
Buffer PBS, 0.02% Sodium azide and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Supplier Images
anti-Integrin, alpha 4 (Antigen CD49D, alpha 4 Subunit of VLA-4 Receptor) (ITGA4) antibody (Biotin) Rat anti CD49d (Integrin alpha 4 chain) R1-2
Background publications Holzmann, McIntyre, Weissman: "Identification of a murine Peyer's patch--specific lymphocyte homing receptor as an integrin molecule with an alpha chain homologous to human VLA-4 alpha." in: Cell, Vol. 56, Issue 1, pp. 37-46, 1989 (PubMed).

Holzmann, Weissman: "Peyer's patch-specific lymphocyte homing receptors consist of a VLA-4-like alpha chain associated with either of two integrin beta chains, one of which is novel." in: The EMBO journal, Vol. 8, Issue 6, pp. 1735-41, 1989 (PubMed).

Berlin, Berg, Briskin et al.: "Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1." in: Cell, Vol. 74, Issue 1, pp. 185-95, 1993 (PubMed).

Hosts (105), (104), (46)
Reactivities (156), (119), (37), (6), (5), (4), (1), (1), (1), (1), (1)
Applications (198), (70), (45), (41), (36), (23), (22), (22), (19), (13), (5), (2), (2), (1), (1)
Conjugates (45), (28), (24), (8), (2), (1), (1)
Epitopes (15), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
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