Integrin, alpha 4 (Antigen CD49D, alpha 4 Subunit of VLA-4 Receptor) (ITGA4) antibody

Details for Product No. ABIN114228
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Antigen
Synonyms LOC100219853, CD49D, IA4
Reactivity
Mouse (Murine)
(159), (125), (36), (6), (4), (4), (1), (1), (1), (1), (1)
Host
Rat
(109), (108), (45)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(47), (38), (26), (2), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Immunoprecipitation (IP), Flow Cytometry (FACS)
(205), (73), (46), (46), (36), (25), (23), (20), (20), (16), (5), (2), (2), (1), (1)
Pubmed 1 reference available
Quantity 0.25 mg
Shipping to United States (Change)
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Catalog No. ABIN114228
313.50 $
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Immunogen Peyers Patch HEV binding lymphoma line (TK1)
Clone R1-2
Isotype IgG2b
Specificity This antibody reacts with alpha 4 integrin.
Purification Protein G affinity purified immunoglobulin fraction
Alternative Name CD49d / ITGA4
Background Alpha 4 integrin, which helps to mediate cell-cell and cell-matrix interactions. It combines with beta 1 and beta 7integrin to form VLA-4 and LPAM-1 (Peyers patch homing receptor) respectively. VLA-4 is expressed on most peripheral lymphocytes, thymocytes and monocytes. LPAM-1 is found on peripheral lymphocytes, but few thymocytes. Fibronectin and VCAM-1 act as ligands for both VLA-4 and LPAM-1. LPAM-1 also binds the mucosal vascular addressin MAdCAM-1. (1)
Alternate names: CD49 antigen-like family member D, Integrin alpha-4, Integrin alpha-IV, VLA-4, VLA4
Gene ID 16401
NCBI Accession NP_034706
UniProt Q00651
Research Area CD Antigens, Surface Receptors of Immune Cells, Cell/Tissue Markers, Extracellular Matrix, Signaling
Application Notes Flow cytometry (see protocol). Immunoprecipitation. Immunohistochemistry on frozen sections. Functional assays. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 0. 5-1. 0 µg* of ABIN114228. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (FITC Goat anti-rat IgG (H+L)) at a 1/500 dilution. 9. Incubate the tubes at 4°C for 30-60 minutes. (It is recommended that the tubes are protected from light since most fluorochromes arelight sensitive). 10. Wash 2 times at 4°C in media B. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls).
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS buffer with 0.02% sodium azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Supplier Images
anti-Integrin, alpha 4 (Antigen CD49D, alpha 4 Subunit of VLA-4 Receptor) (ITGA4) antibody Cell Source: TK1 cell line.,.Percentage of cells stained above control: 99.6%
Background publications Berlin, Berg, Briskin et al.: "Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1." in: Cell, Vol. 74, Issue 1, pp. 185-95, 1993 (PubMed).

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