Leptin (LEP) antibody

Details for Product No. ABIN114695
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Antigen
Synonyms ob, obese, LEPD, OB, OBS
Reactivity
Human
(191), (108), (84), (27), (15), (12), (3), (1), (1), (1), (1)
Host
Mouse
(208), (70), (12), (6), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(32), (7), (6), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application
ELISA, Western Blotting (WB)
(194), (180), (39), (32), (30), (23), (22), (16), (13), (10), (8), (6), (5), (3), (2), (2), (2), (2), (2), (2), (2), (1)
Pubmed 6 references available
Quantity 0.1 mg
Shipping to United States (Change)
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Catalog No. ABIN114695
533.50 $
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Immunogen The Human Leptin is a recombinant protein produced in E.coli.
Clone LEP-02
Isotype IgG1
Specificity The Anti Human Leptin Antibody, Clone LEP-02 is a Mouse monoclonal antibody against Human recombinant Human Leptin. It does not react with Mouse.
Purification Affinity Chromatography on Immobilized Protein G
Alternative Name Leptin
Background Leptin, the product of the ob (obese) gene, is a single-chain 16 kDa protein consisting of 146 amino acid residues. Leptin is produced mainly in the adipose tissue, and is considered to play an important role in appetite control, fat metabolism and body weight regulation. It targets the central nervous system, particularly hypothalamus, affecting food intake. The primary effect of leptin appears to be mediated by leptin receptors expressed mainly in the hypothalamus. In humans, leptin levels correlate with body mass index (BMI) and percentage body fat, and are elevated even in obese individuals. Leptin has a dual action, it decreases the appetite and increases energy consumption, causing more fat to be burned. Leptin is secreted in circadian fashion with nocturnal rise in both lean and obese patients. Mutations of the ob gene resulting in leptin deficiency are the cause of obesity in the ob/ob mice. Endogeneous leptin can normalize their body weight. In contrast, high levels of leptin in obese human subjects point to an insensitivity to endogeneous leptin. Other factors in addition to the amount of body fat appear to regulate leptin action: insulin, glucocorticoids, catecholamines and sex hormones. Studies have shown that leptin may be linked to reproductive function.
Alternate names: LEP, OB, OBS, Obese protein, Obesity factor
Gene ID 3952
NCBI Accession NP_000221
UniProt P41159
Research Area Cardiovascular, Atherosclerosis, Hormones
Application Notes ELISA. Western Blot. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with 0.1 mL of deionized water
Concentration 1.0 mg/mL
Buffer 0.05M phosphate buffer, 0.1M NaCl, pH 7.2. Azide Free
Preservative Azide free
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Prior to reconstitution store at -70°C. Following reconstitution store the antibody (in aliquots) at -20°C for 6 month.
Expiry Date 12 months
Background publications Maffei, Halaas, Ravussin et al.: "Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects." in: Nature medicine, Vol. 1, Issue 11, pp. 1155-61, 1995 (PubMed).

Lönnqvist, Arner, Nordfors et al.: "Overexpression of the obese (ob) gene in adipose tissue of human obese subjects." in: Nature medicine, Vol. 1, Issue 9, pp. 950-3, 1995 (PubMed).

Halaas, Gajiwala, Maffei et al.: "Weight-reducing effects of the plasma protein encoded by the obese gene." in: Science (New York, N.Y.), Vol. 269, Issue 5223, pp. 543-6, 1995 (PubMed).

Zhang, Proenca, Maffei et al.: "Positional cloning of the mouse obese gene and its human homologue." in: Nature, Vol. 372, Issue 6505, pp. 425-32, 1994 (PubMed).

Considine, Sinha, Heiman et al.: "Serum immunoreactive-leptin concentrations in normal-weight and obese humans." in: The New England journal of medicine, Vol. 334, Issue 5, pp. 292-5, 1996 (PubMed).

Ricci, Lee, Russell et al.: "Isoproterenol decreases leptin release from rat and human adipose tissue through posttranscriptional mechanisms." in: American journal of physiology. Endocrinology and metabolism, Vol. 288, Issue 4, pp. E798-804, 2005 (PubMed).

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