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Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (C-Term) antibody

Details for Product No. ABIN116524
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Antigen
Synonyms AI462015, Nfkbi, IKBA, MAD-3, NFKBI, ECI-6/IKBA, RL/IF-1
Epitope
C-Term
(42), (39), (31), (30), (25), (16), (14), (7), (7), (7), (6), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(287), (165), (144), (41), (38), (14), (13), (2)
Host
Rabbit
(262), (31), (6)
Clonality
Polyclonal
Conjugate
Un-conjugated
(9), (7), (7), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Western Blotting (WB)
(220), (109), (96), (47), (42), (40), (37), (13), (9), (7), (5), (4), (2), (1)
Pubmed 2 references available
Quantity 0.1 mL
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Catalog No. ABIN116524
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Immunogen IkBa peptide corresponding to a region near the C-terminus of the Human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Specificity This antibody may react non-specifically with other proteins. This antibody is suitable for the detection by immunoblot of Human, Mouse and Rat IkappaB- alpha. Control Peptide (R1013CP) will compete only with the specific reaction of antiserum with IkappaB- alpha.
Purification Prepared from monospecific antiserum by Delipidation and Defibrination.
Alternative Name NFKBIA / IKBA
Background Three major forms of IKB like molecules have been identified and each is characterised by multiple copies of ankyrin repeats. IKB alpha and IKB beta appear to be the major regulatory forms of IKB in most cells. These proteins interact with p65 or cRel containing forms of NFkB and block nuclear import by masking the nuclear localisation sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IKB. Immunoblotting easily detects the hyperphosphorylated forms of IKB alpha, but not phosphorylated IKB beta. Interestingly, IKB alpha and IKB beta mediate different NFkB responses. IkB alpha appears to control more transient activation of NFkB in response to an inducer, while IKB beta controls a persistent response. Bcl3 interacts with p50 and p52 containing forms of NFkB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IKB is confirmed by immunoblotting.
Alternate names: I kappa B-alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, MAD3, Major histocompatibility complex enhancer-binding protein MAD3, NF-kappa-B inhibitor alpha, NFKBI
Gene ID 4792
NCBI Accession NP_065390.1
UniProt P25963
Application Notes Suitable for Immunoblotting (1/2,000-1/10,000), ELISA (1/20,000-1/100,000) andImmunohistochemistry (1/1,000-1/5,000). Recommended Dilutions: This product was assayed by immunoblot and found to bereactive against IκBa showing a 36 kDa band. Perform all incubations except colordevelopment using TBS supplemented with 0.1% Tween-20 at room temperature. Block themembrane with 5% dry milk for 2 h. Add a 1: 1,000 dilution of the primary antibody to themembrane and incubated for 2 h. Perform washes with buffer 4 times for 5' each. Incubatethe immunoblot with Peroxidase conjugated Affinity Purified Goat anti-Rabbit IgG [H&L] at1/2,000 for 1 h. Wash with TBS only preceded color development. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 80 mg/mL (by Refractometry)
Buffer 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 containing 0.09% (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Baldwin: "The NF-kappa B and I kappa B proteins: new discoveries and insights." in: Annual review of immunology, Vol. 14, Issue 8, pp. 649-83, 1996 (PubMed).

Hoffmann, Levchenko, Scott et al.: "The IkappaB-NF-kappaB signaling module: temporal control and selective gene activation." in: Science (New York, N.Y.), Vol. 298, Issue 5596, pp. 1241-5, 2002 (PubMed).

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