NFKBIA antibody (AA 3-112)

Catalog No. ABIN3043889

There are 4+ publications for this product available. The Rabbit Polyclonal anti-NFKBIA antibody is suitable to detect NFKBIA in samples from Human. It has been validated for WB.

Quick Overview for NFKBIA antibody (AA 3-112) (ABIN3043889)

Target: NFKBIA (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFKBIA antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NFKBIA Antibody

Binding Specificity: AA 3-112

Purpose: Anti-IKB alpha/NFKBIA Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-IKB alpha/NFKBIA Antibody (ABIN3043889). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human IKB alpha recombinant protein (Position: Q3-Q112). Human IKB alpha shares 87% and 86% amino acid (aa) sequence identity with mouse and rat IKB alpha, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-NFKBIA antibody (ABIN3043889)

Chen, Yan, Song, Chen, Wang, Niu, Shi, Zhang, Zhang, Zhai, Zhong, Cheng, Qian, Hao: "Downregulation of TNIP1 Expression Leads to Increased Proliferation of Human Keratinocytes and Severer Psoriasis-Like Conditions in an Imiquimod-Induced Mouse Model of Dermatitis." in: PLoS ONE, Vol. 10, Issue 6, pp. e0127957, (2016) (PubMed).

Wu, Luo, Zhou, Ren: "N-acetylcysteine reduces oxidative stress, nuclear factor‑κB activity and cardiomyocyte apoptosis in heart failure." in: Molecular medicine reports, Vol. 10, Issue 2, pp. 615-24, (2015) (PubMed).

Liu, Yu, Yang, Zhou, Lu, Shi: "Mutations of NFKBIA in biopsy specimens from Hodgkin lymphoma." in: Cancer genetics and cytogenetics, Vol. 197, Issue 2, pp. 152-7, (2010) (PubMed).

Chen, Yin, Wang, Mi, He, Xie, Wang: "Multi-gene targeted antiangiogenic therapies for experimental corneal neovascularization." in: Molecular vision, Vol. 16, pp. 310-9, (2010) (PubMed).

Target Details for NFKBIA

Target: NFKBIA (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA))

Alternative Name: NFKBIA

Background:

Synonyms: NF-kappa-B inhibitor alpha;I-kappa-B-alpha;IkB-alpha;IkappaBalpha;Major histocompatibility complex enhancer-binding protein MAD3;NFKBIA;IKBA, MAD3, NFKBI;

Background: NFKBIA, also called IKBA or MAD-3, is one member of a family of cellular proteins that function to inhibit the NF-κB transcription factor. It is mapped to 14q13.2. NFKBIA inhibits NF-κB by masking the nuclear localization signals (NLS) of NF-κB proteins and keeping them sequestered in an inactive state in the cytoplasm. It moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. The effect of the nonpathogenic bacteria is specific to the SCF complex substrates CTNNB1 and NFKBIA. This may help to explain the beneficial effects of treatment of inflammatory bowel disease with nonpathogenic probiotic enteric organisms. In addition, NFKBIA blocks the ability of NF-κB transcription factors to bind to DNA, which is required for NF-κB's proper functioning.

Sequence Similarities: Belongs to the NF-kappa-B inhibitor family.

Molecular Weight: 39 kDa

Gene ID: 4792

UniProt: P25963

Pathways: NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Maintenance of Protein Location, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, BCR Signaling