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NFKBIA antibody (AA 3-112)

NFKBIA Reactivity: Human, Mouse, Rat WB, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN3043889
  • Target See all NFKBIA Antibodies
    NFKBIA (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA))
    Binding Specificity
    • 53
    • 28
    • 24
    • 21
    • 18
    • 16
    • 9
    • 8
    • 8
    • 8
    • 6
    • 6
    • 6
    • 6
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 3-112
    Reactivity
    • 251
    • 165
    • 132
    • 28
    • 19
    • 17
    • 14
    • 10
    • 7
    • 2
    • 2
    • 2
    • 1
    Human, Mouse, Rat
    Host
    • 217
    • 22
    • 7
    • 5
    Rabbit
    Clonality
    • 204
    • 46
    Polyclonal
    Conjugate
    • 111
    • 21
    • 13
    • 12
    • 9
    • 9
    • 9
    • 9
    • 9
    • 9
    • 9
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    This NFKBIA antibody is un-conjugated
    Application
    • 176
    • 105
    • 67
    • 66
    • 51
    • 38
    • 32
    • 32
    • 22
    • 16
    • 14
    • 12
    • 4
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Purpose
    Rabbit IgG polyclonal antibody for NF-kappa-B inhibitor alpha(NFKBIA) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
    Cross-Reactivity (Details)
    No cross reactivity with other proteins.
    Characteristics
    Rabbit IgG polyclonal antibody for NF-kappa-B inhibitor alpha(NFKBIA) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
    Gene Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
    Protein Name: NF-kappa-B inhibitor alpha
    Purification
    Immunogen affinity purified.
    Immunogen
    E.coli-derived human IKB alpha recombinant protein (Position: Q3-Q112). Human IKB alpha shares 87% and 86% amino acid (aa) sequence identity with mouse and rat IKB alpha, respectively.
    Isotype
    IgG
    Top Product
    Discover our top product NFKBIA Primary Antibody
  • Application Notes
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for IKB alpha is approximately 0.2 ng/lane under reducing conditions.
    IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
    Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
    Comment

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Concentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
  • Chen, Yan, Song, Chen, Wang, Niu, Shi, Zhang, Zhang, Zhai, Zhong, Cheng, Qian, Hao: "Downregulation of TNIP1 Expression Leads to Increased Proliferation of Human Keratinocytes and Severer Psoriasis-Like Conditions in an Imiquimod-Induced Mouse Model of Dermatitis." in: PLoS ONE, Vol. 10, Issue 6, pp. e0127957, (2016) (PubMed).

    Wu, Luo, Zhou, Ren: "N-acetylcysteine reduces oxidative stress, nuclear factor‑κB activity and cardiomyocyte apoptosis in heart failure." in: Molecular medicine reports, Vol. 10, Issue 2, pp. 615-24, (2015) (PubMed).

    Liu, Yu, Yang, Zhou, Lu, Shi: "Mutations of NFKBIA in biopsy specimens from Hodgkin lymphoma." in: Cancer genetics and cytogenetics, Vol. 197, Issue 2, pp. 152-7, (2010) (PubMed).

    Chen, Yin, Wang, Mi, He, Xie, Wang: "Multi-gene targeted antiangiogenic therapies for experimental corneal neovascularization." in: Molecular vision, Vol. 16, pp. 310-9, (2010) (PubMed).

  • Target
    NFKBIA (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA))
    Alternative Name
    NFKBIA (NFKBIA Products)
    Synonyms
    IKBA antibody, MAD-3 antibody, NFKBI antibody, AI462015 antibody, Nfkbi antibody, RL/IF-1 antibody, ECI-6/IKBA antibody, NFKBIA antibody, ikbaB antibody, wu:fb81f08 antibody, zgc:56666 antibody, zgc:77616 antibody, ikbaA antibody, wu:fj16a07 antibody, zgc:85769 antibody, NFKB inhibitor alpha antibody, nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, alpha antibody, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha b antibody, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha a antibody, NFKBIA antibody, Nfkbia antibody, nfkbiab antibody, nfkbiaa antibody
    Background
    NFKBIA, also called IKBA or MAD-3, is one member of a family of cellular proteins that function to inhibit the NF-κB transcription factor. It is mapped to 14q13.2. NFKBIA inhibits NF-κB by masking the nuclear localization signals(NLS) of NF-κB proteins and keeping them sequestered in an inactive state in the cytoplasm. It moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. The effect of the nonpathogenic bacteria is specific to the SCF complex substrates CTNNB1 and NFKBIA. This may help to explain the beneficial effects of treatment of inflammatory bowel disease with nonpathogenic probiotic enteric organisms. In addition, NFKBIA blocks the ability of NF-κB transcription factors to bind to DNA, which is required for NF-κB's proper functioning.

    Synonyms: I kappa B alpha antibody|I(Kappa)B(alpha) antibody|I-kappa-B-alpha antibody|IkappaBalpha antibody|IkB-alpha antibody|IKBA antibody|IKBA_HUMAN antibody|IKBalpha antibody|MAD 3 antibody|MAD3 antibody|Major histocompatibility complex enhancer binding protein MAD3 antibody|Major histocompatibility complex enhancer-binding protein MAD3 antibody|NF kappa B inhibitor alpha antibody|NF-kappa-B inhibitor alpha antibody|NFKBI antibody|NFKBIA antibody|Nuclear factor of kappa light chain gene enhancer in B cells antibody|Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody
    Gene ID
    4792
    UniProt
    P25963
    Pathways
    NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Maintenance of Protein Location, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, BCR Signaling
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