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Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE) (pThr501) antibody

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Synonyms zgc:100844, IKBKE, MGC130907, IKK-E, IKK-i, IKKE, IKKI, AW558201, IKKepsilon, Ikki
(25), (15), (13), (13), (12), (10), (10), (5), (5), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
(107), (44), (23)
(101), (26), (1)
(5), (5), (5), (4), (4), (4), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Enzyme Immunoassay (EIA), Western Blotting (WB)
(103), (58), (40), (19), (15), (6), (4), (2), (2), (1)
Pubmed 2 references available
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Quantity 0.1 mg
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Immunogen IKKE phosphopeptide corresponding to a region of the human protein surrounding pThr501 conjugated to KLH. Genename: IKKe
Sequence R-S-R-L-R-pT-L-A-E-V-L
Specificity This phospho specific polyclonal antibody is specific for phosphorylated pThr501 IKKE. Reactivity with non-phosphorylated IKKE is minimal.
Purification Immunoaffinity Chromatography against the phosphopeptide and cross adsorption against the non-phosphorylated form of the peptide followed by non-adsorption against a non-specific peptide backbone to further reduce non-specific reactivity.
Alternative Name IKBKE / IKKE (IKBKE Antibody Abstract)
Background Nuclear Factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during the activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κB is associated with IκB proteins in the cytoplasm of the cell, which inhibit NF-κB activity. IκB proteins are phosphorylated by an IκB kinase complex consisting of at least three proteins, IKKa, IKKb, and IKKg. Isolated from a cDNA library of LPS-stimulated mouse macrophage cells, a novel molecule in the IKK complex has been recently identified and designated IKKi and/or IKKe. IKKe is required for the activation of NF-κB by mitogens and T cell receptors but not by TNFa or IL-1. LPS increases the IKKe mRNA level in mouse macrophage cell lines. This protein has significant sequence homology with kinase domains of IKKa and IKKb. Overexpression of wild type IKKe in cells phosphorylates Ser32 and Ser36 of IκBa.Synonyms: I kappa-B kinase epsilon, IKK-E, IKK-I, IKK-epsilon, IKKI, Inducible I kappa-B kinase, Inhibitor of nuclear factor kappa-B kinase subunit epsilon, KIAA0151
Gene ID 9641
NCBI Accession NP_001180250
UniProt Q14164
Pathways TLR Signaling
Application Notes This phospho specific polyclonal antibody reacts with phosphorylated human IKKE atpT501 and shows minimal reactivity by Western blot with non-phosphorylated IKKE andminimal reactivity by ELISA against the non-phosphorylated form of the immunizingpeptide. Although not tested, this antibody is likely functional in Immunohistochemistryand Immunoprecipitation.
Restrictions For Research Use only
Concentration 1.15 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Peters, Liao, Maniatis: "IKKepsilon is part of a novel PMA-inducible IkappaB kinase complex." in: Molecular cell, Vol. 5, Issue 3, pp. 513-22, 2000 (PubMed).

Shimada, Kawai, Takeda et al.: "IKK-i, a novel lipopolysaccharide-inducible kinase that is related to IkappaB kinases." in: International immunology, Vol. 11, Issue 8, pp. 1357-62, 1999 (PubMed).

Catalog No. ABIN116937
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