Titration of the IKKE antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100
Restrictions
For Research Use only
Format
Liquid
Buffer
In 1X PBS, pH 7.4, with 0.09 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Aliquot the IKKE antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Target
IKKi/IKKe (IKBKE)
(Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE))
zgc:100844 antibody, IKBKE antibody, MGC130907 antibody, IKK-E antibody, IKK-i antibody, IKKE antibody, IKKI antibody, AW558201 antibody, IKKepsilon antibody, Ikki antibody, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon antibody, inhibitor of nuclear factor kappa B kinase subunit epsilon antibody, inhibitor of nuclear factor kappa B kinase subunit epsilon L homeolog antibody, inhibitor of kappaB kinase epsilon antibody, IKBKE antibody, ikbke antibody, ikbke.L antibody, Ikbke antibody
Background
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).