Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (AA 2440-2457), (pSer2448) antibody

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Synonyms FRAP, FRAP1, FRAP2, RAFT1, RAPT1, 2610315D21Rik, AI327068, Frap1, flat, frap1, tor, wu:fc22h08
AA 2440-2457, pSer2448
(45), (22), (21), (12), (6), (6), (6), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
(243), (153), (145), (63), (36), (24), (5), (2), (1)
(241), (13), (1)
(6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5)
ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(144), (84), (72), (64), (50), (34), (22), (17), (4), (3), (2), (1), (1)
Pubmed 9 references available
Catalog no. ABIN117909
Quantity 0.1 mg
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Immunogen A synthetic peptide corresponding to amino acids 2440-2457 of Human mTOR
Specificity Reactivity occurs with phosphorylated mTOR from Human derived tissues and cells. Reactivity against mTOR from other species has not been determined, however, reactivity with Mouse and Rat is suggested based on protein sequence homologies. Species Reactivity: Tested: Human. Expected from sequence similarity: Mouse and Rat.
Purification Immunoaffinity Chromatography using the immunizing peptide after immobilization to a solid phase.
Alternative Name mTOR / FRAP1
Background Mammalian target of rapamycin (mTOR) is a serine and threonine protein kinase that regulates numerous cellular functions, in particular, the initiation of protein translation. Rapamycin is a natural product macrolide that induces G1 growth arrest in yeast, Drosophila, and mammalian cells. mTOR is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, mTOR is a 245-kD protein referred to as RAFT1 with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of mTOR to FKBP12-rapamycin correlates with the ability of these ligands to inhibit cell cycle progression.
Alternate names: FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, FRAP, FRAP2, Mammalian target of rapamycin, RAPT1, Rapamycin target protein, Serine/threonine-protein kinase mTOR, TOR
Gene ID 2475
NCBI Accession NP_004949.1
UniProt P42345
Application Notes This antibody has been tested for use in Immunohistochemistry (5.0 µg/ml), ELISA(1/15,000-1/65,000) and Western blotting (1/500-1/2,000). Western blotting shows reactivity specific for phospho mTOR detecting a band atapproximately 250 kDa. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.88 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2, containing 0.01% (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer.
Expiry Date 12 months
Supplier Images
anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (AA 2440-2457), (pSer2448) antibody Immunoblotting. Affinity Purified Anti-mTOR pS 2448 (Rabbit) is shown to detect a 250 kDa band (indicated) corresponding to phosphorylated human mTOR present in a 293T whole cell lysates. Cells were serum-starved for 24 hours prior to harvest. ~20ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.
anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (AA 2440-2457), (pSer2448) antibody (2) Immunoblotting. Metabolic and autocrine regulation of the mTOR pathway by beta-cells.
Background publications Zhang, Shu, Hosoi et al.: "Predominant nuclear localization of mammalian target of rapamycin in normal and malignant cells in culture." in: The Journal of biological chemistry, Vol. 277, Issue 31, pp. 28127-34, 2002 (PubMed).

Inoki, Li, Zhu et al.: "TSC2 is phosphorylated and inhibited by Akt and suppresses mTOR signalling." in: Nature cell biology, Vol. 4, Issue 9, pp. 648-57, 2002 (PubMed).

Choi, Bertram, Drenan et al.: "The FKBP12-rapamycin-associated protein (FRAP) is a CLIP-170 kinase." in: EMBO reports, Vol. 3, Issue 10, pp. 988-94, 2002 (PubMed).

Hudson, Liu, Chiang et al.: "Regulation of hypoxia-inducible factor 1alpha expression and function by the mammalian target of rapamycin." in: Molecular and cellular biology, Vol. 22, Issue 20, pp. 7004-14, 2002 (PubMed).

McMahon, Choi, Lin et al.: "The rapamycin-binding domain governs substrate selectivity by the mammalian target of rapamycin." in: Molecular and cellular biology, Vol. 22, Issue 21, pp. 7428-38, 2002 (PubMed).

Nojima, Tokunaga, Eguchi et al.: "The mammalian target of rapamycin (mTOR) partner, raptor, binds the mTOR substrates p70 S6 kinase and 4E-BP1 through their TOR signaling (TOS) motif." in: The Journal of biological chemistry, Vol. 278, Issue 18, pp. 15461-4, 2003 (PubMed).

Kristof, Marks-Konczalik, Billings et al.: "Stimulation of signal transducer and activator of transcription-1 (STAT1)-dependent gene transcription by lipopolysaccharide and interferon-gamma is regulated by mammalian target of rapamycin." in: The Journal of biological chemistry, Vol. 278, Issue 36, pp. 33637-44, 2003 (PubMed).

Chen, Zheng, Foster: "Phospholipase D confers rapamycin resistance in human breast cancer cells." in: Oncogene, Vol. 22, Issue 25, pp. 3937-42, 2003 (PubMed).

Chase, Rao, Hofmann et al.: "1550 nm high contrast grating VCSEL." in: Optics express, Vol. 18, Issue 15, pp. 15461-6, 2010 (PubMed).

Hosts (241), (13), (1)
Reactivities (243), (153), (145), (63), (36), (24), (5), (2), (1)
Applications (144), (84), (72), (64), (50), (34), (22), (17), (4), (3), (2), (1), (1)
Conjugates (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Epitopes (45), (22), (21), (12), (6), (6), (6), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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