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MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (AA 2440-2457)

Details for Product anti-FRAP1 Antibody No. ABIN117909, Supplier: Login to see New
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Synonyms FRAP1, FRAP, FRAP2, RAFT1, RAPT1, 2610315D21Rik, AI327068, Frap1, flat, frap1, tor, wu:fc22h08
AA 2440-2457, pSer2448
(66), (25), (24), (23), (21), (21), (14), (13), (13), (9), (7), (6), (4), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
(340), (159), (147), (7), (2), (2), (2), (2), (1), (1)
(294), (47), (2)
This MTOR antibody is un-conjugated
(7), (7), (7), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (1), (1), (1), (1)
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(222), (112), (87), (67), (55), (45), (33), (22), (8), (7), (7), (3), (2), (2), (2), (2), (2), (1)
Pubmed 9 references available
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Quantity 0.1 mg
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Immunogen A synthetic peptide corresponding to amino acids 2440-2457 of Human mTOR
Specificity Reactivity occurs with phosphorylated mTOR from Human derived tissues and cells. Reactivity against mTOR from other species has not been determined, however, reactivity with Mouse and Rat is suggested based on protein sequence homologies.
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Immunoaffinity Chromatography using the immunizing peptide after immobilization to a solid phase.
Alternative Name mTOR / FRAP1 (FRAP1 Antibody Abstract)
Background Mammalian target of rapamycin (mTOR) is a serine and threonine protein kinase that regulates numerous cellular functions, in particular, the initiation of protein translation. Rapamycin is a natural product macrolide that induces G1 growth arrest in yeast, Drosophila, and mammalian cells. mTOR is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, mTOR is a 245-kD protein referred to as RAFT1 with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of mTOR to FKBP12-rapamycin correlates with the ability of these ligands to inhibit cell cycle progression.Synonyms: FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, FRAP, FRAP2, Mammalian target of rapamycin, RAPT1, Rapamycin target protein, Serine/threonine-protein kinase mTOR, TOR
Gene ID 2475
NCBI Accession NP_004949
UniProt P42345
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes This antibody has been tested for use in Immunohistochemistry (5.0 μg/mL), ELISA(1/15,000-1/65,000) and Western blotting (1/500-1/2,000). Western blotting shows reactivity specific for phospho mTOR detecting a band atapproximately 250 kDa.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.88 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, containing 0.01 % (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
Supplier Images
 image for anti-MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (AA 2440-2457) (ABIN117909) Immunoblotting. Affinity Purified Anti-mTOR pS 2448 (Rabbit) is shown to detect a 250...
 image for anti-MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (AA 2440-2457) (ABIN117909) Immunoblotting. Metabolic and autocrine regulation of the mTOR pathway by beta-cells.
Background publications Chase, Rao, Hofmann et al.: "1550 nm high contrast grating VCSEL." in: Optics express, Vol. 18, Issue 15, pp. 15461-6, 2010 (PubMed).

Chen, Zheng, Foster: "Phospholipase D confers rapamycin resistance in human breast cancer cells." in: Oncogene, Vol. 22, Issue 25, pp. 3937-42, 2003 (PubMed).

Kristof, Marks-Konczalik, Billings et al.: "Stimulation of signal transducer and activator of transcription-1 (STAT1)-dependent gene transcription by lipopolysaccharide and interferon-gamma is regulated by mammalian target of rapamycin." in: The Journal of biological chemistry, Vol. 278, Issue 36, pp. 33637-44, 2003 (PubMed).

Nojima, Tokunaga, Eguchi et al.: "The mammalian target of rapamycin (mTOR) partner, raptor, binds the mTOR substrates p70 S6 kinase and 4E-BP1 through their TOR signaling (TOS) motif." in: The Journal of biological chemistry, Vol. 278, Issue 18, pp. 15461-4, 2003 (PubMed).

McMahon, Choi, Lin et al.: "The rapamycin-binding domain governs substrate selectivity by the mammalian target of rapamycin." in: Molecular and cellular biology, Vol. 22, Issue 21, pp. 7428-38, 2002 (PubMed).

Hudson, Liu, Chiang et al.: "Regulation of hypoxia-inducible factor 1alpha expression and function by the mammalian target of rapamycin." in: Molecular and cellular biology, Vol. 22, Issue 20, pp. 7004-14, 2002 (PubMed).

Choi, Bertram, Drenan et al.: "The FKBP12-rapamycin-associated protein (FRAP) is a CLIP-170 kinase." in: EMBO reports, Vol. 3, Issue 10, pp. 988-94, 2002 (PubMed).

Inoki, Li, Zhu et al.: "TSC2 is phosphorylated and inhibited by Akt and suppresses mTOR signalling." in: Nature cell biology, Vol. 4, Issue 9, pp. 648-57, 2002 (PubMed).

Zhang, Shu, Hosoi et al.: "Predominant nuclear localization of mammalian target of rapamycin in normal and malignant cells in culture." in: The Journal of biological chemistry, Vol. 277, Issue 31, pp. 28127-34, 2002 (PubMed).

Catalog No. ABIN117909
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