Cullin 2 (CUL2) (C-Term), (AA 733-745) antibody

Details for Product No. ABIN117948
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Antigen
Synonyms 1300003D18Rik, 4932411N15Rik, AI327301, mKIAA4106, ATCUL2, F22D16.2, F22D16_2, cullin 2, cul2, Cullin-2, fj48a06, wu:fj48a06, cullin-2, LOC100221330
Epitope
C-Term, AA 733-745
(19), (4), (2), (2), (2), (1), (1), (1)
Reactivity
Human
(68), (37), (21), (14), (14), (1)
Host
Rabbit
(64), (4), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Immunoprecipitation (IP), Western Blotting (WB)
(52), (33), (22), (17), (14), (11), (10), (5), (2), (1), (1)
Pubmed 11 references available
Catalog no. ABIN117948
Quantity 0.1 mL
Price
390.50 $   Plus shipping costs $45.00
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Synthetic peptide corresponding to amino acids 733-745 of Human Cul2 (C-terminus) coupled to KLH.
Sequence Human Cullin 2 (745 aa 86.9 kDa): 1 mslkprvvdf detwnklltt ikavvmleyv eratwndrfs diyalcvayp eplgerlyte 61 tkiflenhvr hlhkrvlese eqvlvmyhry weeyskgady mdclyrylnt qfikknklte 121 adlqygyggv dmneplmeig elaldmwrkl mveplqaili rmllreiknd rggedpnqkv 181 ihgvinsfvh veqykkkfpl kfyqeifesp fltetgeyyk qeasnllqes ncsqymekvl 241 grlkdeeirc rkylhpssyt kvihecqqrm vadhlqflha echniirqek kndmanmyvl 301 lravstglph miqelqnhih deglratsnl tqenmptlfv esvlevhgkf vqlintvlng 361 dqhfmsaldk altsvvnyre pksvckapel lakycdnllk ksakgmtene vedrltsfit 421 vfkyiddkdv fqkfyarmla krlihglsms mdseeamink lkqacgyeft sklhrmytdm 481 svsadlnnkf nnfiknqdtv idlgisfqiy vlqagawplt qapsstfaip qeleksvqmf 541 elfysqhfsg rkltwlhylc tgevkmnylg kpyvamvtty qmavllafnn setvsykelq 601 dstqmnekel tktikslldv kminhdseke didaessfsl nmnfsskrtk fkittsmqkd 661 tpqemeqtrs avdedrkmyl qaaivrimka rkvlrhnali qevisqsrar fnpsismikk 721 cievlidkqy iersqasade ysyva
Specificity This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human Cullin 2. Cross reactivity with Cul2 from other sources has not been determined.
Purification Delipidation and defibrination.
Alternative Name Cullin-2
Background Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 2 is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul2 serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul2 may also contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Cul2 is part of the SCF complex consisting of CUL1, RBX1, SKP1 and SKP2, where it interacts directly with SKP1, SKP2 and RBX1. Cul2 also interacts with RNF7 and is part of a complex with TIP120A/CAND1 and RBX1. The unneddylated form interacts with TIP120A/CAND1 and the interaction negatively regulates the association with SKP1 in the SCF complex.
Alternate names: CUL-2, CUL2
Gene ID 8453
NCBI Accession NP_001185706.1
UniProt Q13617
Application Notes Western blot: This antibody reacts with human Cul2 (1/500-1/1,000, An 86.9 kDa bandcorresponding to human Cul2 is detected). Most cell lines expressing Cul2 can be used asa positive control). Immunoprecipitation: The antibody immunoprecipitates in vitro translated product andprotein from cell lysates (using HeLa or NIH-3T3). ELISA (1/2,000-1/10,000). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 85.0 mg/mL (by Refractometry)
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8°C for up to one month or at -20°C for longer.
Expiry Date 12 months
Supplier Images
anti-Cullin 2 (CUL2) (C-Term), (AA 733-745) antibody Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a, amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch, see references below.
Background publications Kipreos, Lander, Wing et al.: "cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family." in: Cell, Vol. 85, Issue 6, pp. 829-39, 1996 (PubMed).

Mathias, Johnson, Winey et al.: "Cdc53p acts in concert with Cdc4p and Cdc34p to control the G1-to-S-phase transition and identifies a conserved family of proteins." in: Molecular and cellular biology, Vol. 16, Issue 12, pp. 6634-43, 1997 (PubMed).

Pause, Lee, Worrell et al.: "The von Hippel-Lindau tumor-suppressor gene product forms a stable complex with human CUL-2, a member of the Cdc53 family of proteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 6, pp. 2156-61, 1997 (PubMed).

Michel, Xiong: "Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A." in: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, Vol. 9, Issue 6, pp. 435-49, 1998 (PubMed).

Wada, Yeh, Kamitani: "Identification of NEDD8-conjugation site in human cullin-2." in: Biochemical and biophysical research communications, Vol. 257, Issue 1, pp. 100-5, 1999 (PubMed).

Ohta, Michel, Schottelius et al.: "ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity." in: Molecular cell, Vol. 3, Issue 4, pp. 535-41, 1999 (PubMed).

Feng, Zhong, Punkosdy et al.: "CUL-2 is required for the G1-to-S-phase transition and mitotic chromosome condensation in Caenorhabditis elegans." in: Nature cell biology, Vol. 1, Issue 8, pp. 486-92, 2000 (PubMed).

Jentsch, Pyrowolakis: "Ubiquitin and its kin: how close are the family ties?" in: Trends in cell biology, Vol. 10, Issue 8, pp. 335-42, 2000 (PubMed).

Kamura, Sato, Iwai et al.: "Activation of HIF1alpha ubiquitination by a reconstituted von Hippel-Lindau (VHL) tumor suppressor complex." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 19, pp. 10430-5, 2000 (PubMed).

Nau: "[On commotion and on prostitution]." in: Revue médicale suisse, Vol. 6, Issue 270, pp. 2156-7, 2010 (PubMed).

General Weck, Pause, Pinzer: "[Spinal meningioma as differential diagnosis of diabetic polyneuropathy]" in: Deutsche medizinische Wochenschrift (1946), Vol. 126, Issue 20, pp. 590-2, 2001 (PubMed).

Hosts (64), (4), (3)
Reactivities (68), (37), (21), (14), (14), (1)
Applications (52), (33), (22), (17), (14), (11), (10), (5), (2), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (19), (4), (2), (2), (2), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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