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Checkpoint Kinase 2 (CHEK2) (AA 64-73), (pThr68) antibody

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Synonyms CHK-2, CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53, hCds1, Cds1, HUCDS1, Rad53, Chk2, fa66f08, wu:fa66f08, zgc:55865
AA 64-73, pThr68
(50), (42), (40), (33), (24), (15), (13), (13), (12), (11), (10), (8), (7), (7), (6), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
(419), (160), (160), (8), (8), (6), (5), (4), (1), (1), (1)
(335), (89), (8), (1), (1)
(7), (7), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (1), (1), (1), (1), (1)
Enzyme Immunoassay (EIA), Western Blotting (WB)
(341), (115), (107), (70), (67), (57), (37), (29), (24), (6), (4), (2), (1)
Pubmed 3 references available
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Quantity 0.1 mg
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 64-73 of Human CHK2.
Sequence Human CHK2, 543 aa, predicted MW 60.9 kDa 1 msresdveaq qshgssacsq phgsvtqsqg sssqsqgiss sststmpnss qsshsssgtl 61 ssletvstqe lysipedqep edqepeeptp apwarlwalq dgfanlecvn dnywfgrdks 121 ceycfdepll krtdkyrtys kkhfrifrev gpknsyiayi edhsgngtfv ntelvgkgkr 181 rplnnnseia lslsrnkvfv ffdltvddqs vypkalrdey imsktlgsga cgevklafer 241 ktckkvaiki iskrkfaigs areadpalnv eteieilkkl nhpciikikn ffdaedyyiv 301 lelmeggelf dkvvgnkrlk eatcklyfyq mllavqylhe ngiihrdlkp envllssqee 361 dclikitdfg hskilgetsl mrtlcgtpty lapevlvsvg tagynravdc wslgvilfic 421 lsgyppfseh rtqvslkdqi tsgkynfipe vwaevsekal dlvkkllvvd pkarftteea 481 lrhpwlqded mkrkfqdlls eenestalpq vlaqpstsrk rpregeaega ettkrpavca 541 avl
Specificity This affinity purified antibody is directed against the phosphorylated form of human CHK2 at the pT68 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against human CHK2 pT68 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human CHK2 is minimal by ELISA. The antibody does not cross-react with Chk2 phosphorylated at other sites. A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100 % homology for the immunogen sequence. Cross reactivity with CHK2 protein from mouse and rat may occur as sequence homology varies by one amino acid residues in this sequence (90 % homology). Cross reactivity with CHK2 homologues from other sources has not been determined.
Purification Immunoaffinity chromatography.
Alternative Name CHK2 (CHEK2 Antibody Abstract)
Background CHK2 is a serine/threonine-protein kinase involved in the control of cell cycle checkpoints and may also participate in transduction of the DNA damage and replicational stress signals. CHK2 is the mammalian ortholog of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases. The amino-terminal domain of CHK2 contains a series of seven serine and threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50 and Thr68) followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases. Indeed, after DNA damage by ionizing radiation (IR), UV irradiation or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR. The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 on residues Thr383 and Thr387 in the activation loop of the kinase domain. CHK2 inhibits CDC25C phosphatase by phosphorylating it on Ser-216, preventing the entry into mitosis. This kinase may have a role in meiosis as well. Kinase activity is up regulated by autophosphorylation and the protein is rapidly phosphorylated in response to DNA damage and to replication block. CHK2 shows a nuclear localization and is highly expressed in testis, spleen, colon and peripheral blood leukocytes. Low levels of expression are found in other tissues. Defects in CHEK2 are associated with Li-Fraumeni syndrome (LFS), a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53. Defects in CHEK2 are also found in some patients with prostate cancer (CaP) or osteosarcoma (OSRC). Substantial amino acid variants exist in some cancer tissues. Numerous truncated splice variants exist for this protein.Synonyms: CHEK-2, CHEK2, CHK-2, CHK2 checkpoint homolog, Cds1, RAD53, Serine/threonine-protein kinase Chk2
Gene ID 11200
NCBI Accession NP_001005735
UniProt O96017
Pathways p53 Signaling, Apoptosis, Cell Division Cycle
Application Notes Suitable for use in ELISA (1/10,000-1/50,000) and Western blot (1/200-1/2,000). Expect aband approx. 61 kDa in size corresponding to CHK2 by Western blotting in the appropriate celllysate or extract. Less than 1 % reactivity is observed against the non-phosphorylated formof the immunizing peptide.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Store undiluted at 2-8 °C. DO NOT FREEZE!
Supplier Images
Western Blotting (WB) image for anti-Checkpoint Kinase 2 (CHEK2) (AA 64-73), (pThr68) antibody (ABIN117975) Figure. Western blot using Affinity Purified
Background publications Brown, Lee, Schwarz et al.: "A human Cds1-related kinase that functions downstream of ATM protein in the cellular response to DNA damage." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 7, pp. 3745-50, 1999 (PubMed).

Blasina, de Weyer, Laus et al.: "A human homologue of the checkpoint kinase Cds1 directly inhibits Cdc25 phosphatase." in: Current biology : CB, Vol. 9, Issue 1, pp. 1-10, 1999 (PubMed).

Matsuoka, Huang, Elledge: "Linkage of ATM to cell cycle regulation by the Chk2 protein kinase." in: Science (New York, N.Y.), Vol. 282, Issue 5395, pp. 1893-7, 1998 (PubMed).

Catalog No. ABIN117975
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