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Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (AA 2-32) antibody

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Synonyms mif, Mif, gif, glif, mmif, LOC100136498, LOC100284350, LOC100284546, GIF, Glif, GLIF, MMIF
AA 2-32
(28), (10), (9), (9), (8), (5), (4), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
(147), (36), (36), (5), (5), (3), (2), (1), (1)
(104), (37), (21), (5), (1), (1)
(13), (9), (6), (1), (1), (1), (1), (1), (1)
Enzyme Immunoassay (EIA), Western Blotting (WB)
(133), (97), (30), (28), (16), (8), (6), (4), (4), (4), (4), (2), (2), (1), (1), (1), (1)
Pubmed 11 references available
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Quantity 0.5 mg
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Availability Will be delivered in 6 to 8 Business Days
Immunogen This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to aa 2-32 of Human MIF conjugated to keyhole limpet hemocyanin (KLH). MIF is a proinflammatory cytokine that plays an important role in systemic inflammatory events.
Sequence P-M-F-I-V-N-T-N-V-P-R-A-S-V-P-D-G-F-L-S-E-L-T-Q-Q-L-A-Q-A-T-G
Specificity This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum. The antibody is directed against the 12,300 MW human MIF protein and is useful in determining its presence in various assays. If neutralization experiments are performed for human MIF activity in bioassays, it is recommended to incubate the sample with a 1:500 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal chicken IgG is recommended. If FACS analysis experiments are performed for human MIF caution should be exhibited as the F( c) domain of the chicken IgG molecule may interact with cells non-specifically. Endotoxin Content: < 10 pg/μl by LAL method.
Purification Multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis
Alternative Name MIF (MIF Antibody Abstract)
Background Cytokines play an important role in inflammation and immunity. Macrophage migration inhibitory factor (MIF) was one of the first cytokine activities described and is a proinflammatory cytokine that plays an important role in systemic inflammatory events. MIF's cytokine activity was initially described as a T cell-derived factor that inhibited the random migration of macrophages, hence its name. Since it was cloned and expressed in pure form, MIF's activities have been established to play many roles in development and various disease states. For example, MIF is released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counterregulates the immunosuppressive effects of steroids on immune cell activation and cytokine production. In in vitro experiments MIF is significantly upregulated by the stimulation of lipopolysaccharide (LPS) using 10 pg/mL to 10 ng/mL of LPS and reaches the maximum 12 h after the stimulation. MIF also prevents cleavage of Bax into an 18- kDa active fragment, and, consequently, reduces activation of the critical effector caspase 3, suggesting that MIF inhibits apoptosis pathways proximal to mitochondria activation and is therefore a survival factor. And, MIF is also known to exhibit enzymatic activities. A pathologic role for MIF has been described in many conditions including arthritis, asthma, and inflammatory bowel disease, and may also play a role in the control of cell growth in certain cancers. Consequently MIF is suggested to be a potential therapeutic target for human diseases.Synonyms: GLIF, Glycosylation-inhibiting factor, MMIF, Macrophage migration inhibitory factor, Phenylpyruvate tautomerase
Gene ID 4282
NCBI Accession NP_002406
UniProt P14174
Research Area Immunology, Innate Immunity, Cytokines, Inflammation
Application Notes This IgY fraction antibody of anti-Human MacrophageMigration Inhibitory Factor (MIF) has been tested for use in ELISA and Immunoblotting. Although not tested, this antibody may also be useful for Neutralization assays,Immunohistochemistry and Flow Cytometry. The antibody recognizes 12,300 MW maturehuman MIF. The MIF gene encodesa protein of 115 aa. The initiating methionine is cleaved leaving a mature protein of 114 aa. Reactivity in other immunoassays is unknown. Immunoblot: Using IRDYE800 conjugated Goat-a-Chicken IgG [H&L] MX10. A workingdilution range of 1: 500-1: 1,000 is suggested for this application to detect human MIF fromsupernatants or lysates of 2 x 10^6 endotoxin-stimulated human peripheral bloodmononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1 % (v/v) human serumplus 10 ng/mL E. coli LPS. This product has been assayed by ELISA against human MIF using HRP ConjugatedGoat-a-Chicken IgG [H&L] MX10 and ABTS as a substrate for 30 minutes at roomtemperature. A working dilution range of 1: 1,000-1: 2,000 is suggested for this product. For use in ELISAformats, this antibody is best used as the second antibody in combination with amonoclonal antibody as a capture antibody.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 0.1 mL of deionized water (or equivalent).
Concentration 5.0 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Storage 4 °C/-20 °C
Storage Comment Store vial at 2-8 °C prior to restoration. For extended storage mix with glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid.
Supplier Images
Western Blotting (WB) image for anti-Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (AA 2-32) antibody (ABIN118028) Western blot analysis of.,.IgY fraction of Chicken-anti-Human MIF.,.polyclonal antibo...
Background publications Kleemann, Hausser, Geiger et al.: "Intracellular action of the cytokine MIF to modulate AP-1 activity and the cell cycle through Jab1." in: Nature, Vol. 408, Issue 6809, pp. 211-6, 2000 (PubMed).

Onodera, Kaneda, Mizue et al.: "Macrophage migration inhibitory factor up-regulates expression of matrix metalloproteinases in synovial fibroblasts of rheumatoid arthritis." in: The Journal of biological chemistry, Vol. 275, Issue 1, pp. 444-50, 2000 (PubMed).

Kleemann, Kapurniotu, Frank et al.: "Disulfide analysis reveals a role for macrophage migration inhibitory factor (MIF) as thiol-protein oxidoreductase." in: Journal of molecular biology, Vol. 280, Issue 1, pp. 85-102, 1998 (PubMed).

Bucala: "Neuroimmunomodulation by macrophage migration inhibitory factor (MIF)." in: Annals of the New York Academy of Sciences, Vol. 840, pp. 74-82, 1998 (PubMed).

Bernhagen, Calandra, Bucala: "Regulation of the immune response by macrophage migration inhibitory factor: biological and structural features." in: Journal of molecular medicine (Berlin, Germany), Vol. 76, Issue 3-4, pp. 151-61, 1998 (PubMed).

Rosengren, Bucala, Aman et al.: "The immunoregulatory mediator macrophage migration inhibitory factor (MIF) catalyzes a tautomerization reaction." in: Molecular medicine (Cambridge, Mass.), Vol. 2, Issue 1, pp. 143-9, 1997 (PubMed).

Claret, Hibi, Dhut et al.: "A new group of conserved coactivators that increase the specificity of AP-1 transcription factors." in: Nature, Vol. 383, Issue 6599, pp. 453-7, 1996 (PubMed).

Bernhagen, Calandra, Mitchell et al.: "MIF is a pituitary-derived cytokine that potentiates lethal endotoxaemia." in: Nature, Vol. 365, Issue 6448, pp. 756-9, 1993 (PubMed).

Mitchell, Bacher, Bernhagen et al.: "Cloning and characterization of the gene for mouse macrophage migration inhibitory factor (MIF)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 8, pp. 3863-70, 1995 (PubMed).

Calandra, Bernhagen, Metz et al.: "MIF as a glucocorticoid-induced modulator of cytokine production." in: Nature, Vol. 377, Issue 6544, pp. 68-71, 1995 (PubMed).

Weiser, Temple, Witek-Giannotti et al.: "Molecular cloning of a cDNA encoding a human macrophage migration inhibitory factor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 19, pp. 7522-6, 1989 (PubMed).

Catalog No. ABIN118028
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