MIF antibody (C-Term)

Catalog No. ABIN185406

The Goat Polyclonal anti-MIF antibody (ABIN185406) specifically detects MIF in WB, IHC and ELISA. The antibody is reactive with Human samples.

Quick Overview for MIF antibody (C-Term) (ABIN185406)

Target: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MIF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-MIF Antibody

Binding Specificity: C-Term

Purpose: MIF

Sequence: NAANVGWNNS TFA

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-NAANVGWNNSTFA, from the C Terminus of the protein sequence according to NP_002406.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Thymus. Recommended concentration: 5 μg/mL.

Western Blot: Approx 13 kDa band observed in Human Lymph Node, and Thymus lysates and similar bands are observed in lysates of cell lines Daudi and Jurkat (calculated MW of 12.5 kDa according to NP_002406.1). Recommended concentration: 0.01-0.03 μg/mL. Pri

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MIF

Target: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Alternative Name: MIF

Background: MIF, macrophage migration inhibitory factor (glycosylation-inhibiting factor) , HGNC:7097, GIF, GLIF, MMIF, glycosylation-inhibiting factor, phenylpyruvate tautomerase, macrophage migration inhibitory factor

Gene ID: 4282

NCBI Accession: NP_002406

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling